Interleukins’ expression profile changes in granulosa cells of preovulatory follicles during the postpartum period in dairy cows

Warma, Aly; Descarreaux, Marianne; Chorfi, Younès; Dupras, Robert; Rémillard, Roxane; Ndiaye, Kalidou

doi:10.1016/j.cytox.2020.100022

Cited by 6 publications

(8 citation statements)

References 45 publications

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“…Moreover, GCs prevent oocyte abnormal nuclear and cytoplasmic maturation [ 47 , 48 ]. The current findings corroborated with previously published data that immune signaling molecules, namely cytokines and chemokines, are differentially expressed and secreted at a low level by the GCs in the basal condition to support normal follicular maturation [ 2 – 5 , 10 , 11 , 14 ]. The gonadotropins-dependent optimal levels of cytokines and chemokines, their complicated signaling network, and the direction of the response are the hallmarks of many processes in reproductive physiology.…”

Section: Discussionsupporting

confidence: 92%

“…The gonadotropins-dependent optimal levels of cytokines and chemokines, their complicated signaling network, and the direction of the response are the hallmarks of many processes in reproductive physiology. These include antral follicle and POF formation and maturation, ovulation, CL formation, luteolysis, menstruation, and implantation [ 2 – 5 , 10 , 11 , 13 , 14 , 40 , 43 , 49 ]. Additionally, we have demonstrated that inhibition of NRG1 expression in GCs promotes uncontrolled cytokines and chemokines expression and secretion [ 29 – 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…IL-1α, IL-1β, ILs-6, and TNFα are among the cytokines that are most commonly found to increase at the intra-organ and systemic levels during inflammaging [ 70 ]. One cause of decreased fertility during the post-partum period with a prolonged anovulatory status is a higher concentration of inflammatory cytokines [ 14 , 70 ]. Thus, these results may have clinical implications.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines and chemokines create a chemotactic immune and non-immune gradient in the follicle [ 2 – 4 , 12 , 13 ]. In turn, these cytokines and chemokines govern locally follicular cell fate and homeostasis by regulating cell survival, death, proliferation, and differentiation [ 3 , 10 , 11 , 13 , 14 ]. Given these pivotal roles and their ease of detection in follicular fluid, cytokines and chemokines have been considered attractive biomarkers of oocyte maturational status and successfully assisted reproductive outcomes [ 5 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Banerjee

Mishra

et al. 2022

J Ovarian Res

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Background Granulosa cells (GCs) are multilayered somatic cells within the follicle that provide physical support and microenvironment for the developing oocyte. In recent years, the role of Neuregulin-1 (NRG1), a member of the EGF-like factor family, has received considerable attention due to its neurodevelopmental and cardiac function. However, the exact physiological role of NRG1 in GC is mainly unknown. In order to confirm that NRG1 plays a regulatory role in rat GC functions, endogenous NRG1-knockdown studies were carried out in GCs using RNA interference methodology. Results Knockdown of NRG1 in GCs resulted in the enhanced expression and secretion of the cytokines and chemokines. In addition, the phosphorylation of PI3K/Akt/ERK1/2 was significantly low in GCs under these experimental conditions. Moreover, in vitro experimental studies suggest that tumor necrosis factor-α (TNFα) treatment causes the physical destruction of GCs by activating caspase-3/7 activity. In contrast, exogenous NRG1 co-treatment of GCs delayed the onset of TNFα-induced apoptosis and inhibited the activation of caspase-3/7 activity. Furthermore, current experimental studies suggest that gonadotropins promote differential expression of NRG1 and ErbB3 receptors in GCs of the antral follicle. Interestingly, NRG1 and ErbB3 were intensely co-localized in the mural and cumulus GCs and cumulus-oocyte complex of pre-ovulatory follicles in the estrus stage. Conclusions The present studies suggest that gonadotropins-dependent NRG1-signaling in GCs may require the balance of the cytokines and chemokines expression and secretion, ultimately which may be supporting the follicular maturation and oocyte competence for ovulation and preventing follicular atresia.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Banerjee

Mishra

et al. 2022

J Ovarian Res

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“…Thus, under physiological conditions, TNF-α, IL-1α, IL-1β and IL-6 are continuously expressed in the testis, without inducing any inflammation, but regulating spermatogenesis on different levels [185,[189][190][191][192][193][194][195][196][197]. In the ovary, cytokines TNF-α, interleukins IL-1β -6, -8 and others [198][199][200] promote follicular growth, steroidogenesis, recruitment and activation of leukocytes necessary for ovulation and tissue remodeling during ovulation, luteinization and luteolysis [198][199][200][201]. When upregulated, they negatively affect spermatogenesis and oogenesis, leading to infertility inducing germ cell apoptosis, impairment of blood barriers, immune cell infiltration and the production of autoantibodies [202][203][204][205][206].…”

Section: Tam Receptors As Negative Regulators Of Inflammation: Two Possible Explanationsmentioning

confidence: 99%

MERTK-Mediated LC3-Associated Phagocytosis (LAP) of Apoptotic Substrates in Blood-Separated Tissues: Retina, Testis, Ovarian Follicles

Yéfimova

Ravel

Rolland

et al. 2021

Cells

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Timely and efficient elimination of apoptotic substrates, continuously produced during one’s lifespan, is a vital need for all tissues of the body. This task is achieved by cells endowed with phagocytic activity. In blood-separated tissues such as the retina, the testis and the ovaries, the resident cells of epithelial origin as retinal pigmented epithelial cells (RPE), testis Sertoli cells and ovarian granulosa cells (GC) provide phagocytic cleaning of apoptotic cells and cell membranes. Disruption of this process leads to functional ablation as blindness in the retina and compromised fertility in males and females. To ensure the efficient elimination of apoptotic substrates, RPE, Sertoli cells and GC combine various mechanisms allowing maintenance of tissue homeostasis and avoiding acute inflammation, tissue disorganization and functional ablation. In tight cooperation with other phagocytosis receptors, MERTK—a member of the TAM family of receptor tyrosine kinases (RTK)—plays a pivotal role in apoptotic substrate cleaning from the retina, the testis and the ovaries through unconventional autophagy-assisted phagocytosis process LAP (LC3-associated phagocytosis). In this review, we focus on the interplay between TAM RTKs, autophagy-related proteins, LAP, and Toll-like receptors (TLR), as well as the regulatory mechanisms allowing these components to sustain tissue homeostasis and prevent functional ablation of the retina, the testis and the ovaries.

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Sheep litter size heredity basis using genome‐wide selective analysis

Cui,

Wang,

et al. 2024

Reprod Domestic Animals

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Sheep are important herbivorous domestic animal globally, and the Chinese indigenous sheep breed has a multitude of economically significant variations due to the diverse geographical and ecological conditions. In particular, certain native breeds exhibit a visible high litter size phenotype due to the selection pressure of natural and artificial for thousands of years, offering an ideal animal model for investigating sheep's fecundity. In this study, selective signal analysis was performed on public whole‐genome sequencing data from 60 sheep across eight breeds to identify candidate genes related to litter size. Results revealed that a total of 34,065,017 single‐nucleotide polymorphisms (SNPs) were identified from all sheep, and 65 candidate genes (CDGs) were pinpointed from the top 1% of interacted windows and SNPs between the pairwise fixation index (FST, >0.149543) and cross‐population extended haplotype homozygosity (XP‐EHH, >0.701551). A total of 41 CDGs (e.g. VRTN, EYA2 and MCPH1) were annotated to 576 GO terms, of which seven terms were directly linked to follicular and embryonic development (e.g. TBXT, BMPR1B, and BMP2). In addition, 73 KEGG pathways were enriched by 21 CDGs (e.g. ENTPD5, ABCD4 and RXFP2), mainly related to Hippo (TCF4, BMPR1B and BMP2), TGF‐β (BMPR1B and BMP2), PI3K‐Akt (ITGB4, IL4R and PPP2R5A) and Jak–STAT signalling pathways (IL20RA and IL4R). Notably, a series of CDGs was under strong selection in sheep with high litter size traits. These findings result could improve the comprehension of the genetic underpinnings of sheep litter size. Furthermore, it provides valuable CDGS for future molecular breeding.

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Interleukins’ expression profile changes in granulosa cells of preovulatory follicles during the postpartum period in dairy cows

Cited by 6 publications

References 45 publications

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

MERTK-Mediated LC3-Associated Phagocytosis (LAP) of Apoptotic Substrates in Blood-Separated Tissues: Retina, Testis, Ovarian Follicles

Sheep litter size heredity basis using genome‐wide selective analysis

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