Intermethod variation in detection of human papillomavirus DNA in cervical smears

Smits, Henk L.; Bollen, Liesbeth J. M.; Tjong-A-Hung, Steven P.; Vonk, J. Arie; Velden, J. van der; Kate, Fiebo J.W. ten; Kaan, J. A.; Mol, Ben Willem J.; Schegget, Jan ter

doi:10.1128/jcm.33.10.2631-2636.1995

Cited by 56 publications

(26 citation statements)

References 22 publications

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“…For our study population, these results could be due to an overgrading of the Pap smears, a transient HPV infection (in some cases the sample for HPV testing was collected 2–3 months after the Pap smear), or a lower degree of sensitivity of our PCR conditions (100 HPV copies). The intermethod and interlaboratory variations in the detection of HPV DNA in cervical samples are a matter of concern, and the object of several studies [13, 23–25]. The degenerated primer pair MY09/MY11 also used in this study has been shown to be superior to the other widely used consensus primer pair GP5+/GP6+ in detecting multiple infections and types 53 and 61 but less efficient in detecting type 35 [24], leading some investigators to recommend multiple PCR primer sets for optimal detection of HPVs [13].…”

Section: Discussionmentioning

confidence: 89%

Genital Human Papillomavirus Types in Immunocompetent and Immunodepressed Women in Northeast Italy: Prevalence and Cytomorphological Correlations

Mistro

Bonaldi

Bertorelle

et al. 2001

J Low Genital Tract Dis

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H uman papillomaviruses (HPVs) are etiologically related to the development of cervical cancer [1] and the large majority of the squamous cell abnormalities that precede squamous cell carcinoma of the cervix [2]. More than 80 different HPV types exist; 35-40 types infect the anogenital area. The relative risk associations of 15 common anogenital types, in which types 6/11, 42, 43, and 44 were defined as "low risk," types 31, 33, 35, 51, 52, and 58 as "intermediate risk," and types 16, 18, 45, and 56 as "high risk," show that the presence of an oncogenic HPV type confers a higher rel-Abstract Objective. We evaluated the prevalence of genital human papillomavirus (HPV) types in correlation with cytomorphological findings in patients at different risk for cervical intraepithelial neoplasia living in northeast Italy.Methods. Exfoliated cervicovaginal cells from 943 women, who were divided into three groups, were analyzed by polymerase chain reaction.Results. Overall, HPV prevalence rates were 7%, 38%, and 52%, respectively. The single most frequent type was HPV 16 (18%), followed by types 6, 31, 53, 58, 61, and novel/unidentified (5-7%); other types had a frequency Ͻ 5%. Infection with multiple types was present in 12%. In HIV-infected women, HPV infection was correlated with lower CD4 level and higher viral load; HGSILs were correlated only with a lower CD4 count, and no correlations were found for LGSILs.Conclusions. HGSILs were associated with high-risk types, mainly HPV 16 (40%). LGSILs, instead, were associated with a broad spectrum of low-risk and high-risk types.

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Section: Discussionmentioning

confidence: 89%

Genital Human Papillomavirus Types in Immunocompetent and Immunodepressed Women in Northeast Italy: Prevalence and Cytomorphological Correlations

Mistro

Bonaldi

Bertorelle

et al. 2001

J Low Genital Tract Dis

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“…Genomic mechanisms in the pathway of neoplastic transformation of epithelial cells in the uterine cervix have been defined and described in detail elsewhere (4)(5)(6)(7) . In the meantime, the prognostic relevance of HPV status for the clinical risk of potential progression in cervical intraepithelial lesions has been widely accepted (16,17,19) . However there exist contradictory data concerning the influence of HPV DNA as well as HPV genotypes concerning the clinical outcome of cervical cancer patients (8)(9)(10)(11)(12) .…”

Section: Discussionmentioning

confidence: 99%

“…Forty-step cycles were performed and the amplification product was analyzed on a 2% ethidium bromide-stained agarose gel after phenolchloroform extraction and ethanol precipitation. To determine whether samples were adequate for HPV detection by PCR amplification, PCR amplification of a 184-bp fragment of A-myb gene was performed as described before (16). Between patient samples, negative controls (water only) were used throughout the extraction procedure.…”

Section: Hpv Dna Analysismentioning

confidence: 99%

The presence of HPV DNA in cervical cancer: Correlation with clinico-pathologic parameters and prognostic significance: 10 years experience at the Department of Obstetrics and Gynecology of the Mainz University

Pilch¹,

Günzel²,

Schäffer³

et al. 2001

Int J Gynecol Cancer

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The objective of this study was to assess whether the presence of human papillomavirus (HPV) DNA and/or several genotypes of HPV DNA in cervical cancer are correlated with several clinicopathologic parameters of well-defined prognostic significance and whether virologic parameters are predictors of long-term survival in cancer patients. Two hundred twenty three cases of cervical cancer patients included in this retrospective study underwent follow-up evaluation. Survival and cause of death were examined for 204 (91.4%) patients, with a mean follow-up time of 4.4 years. HPV DNA was detected using the highly sensitive polymerase chain reaction (PCR) method followed by HPV DNA sequencing for HPV genotyping. These results were correlated with well-defined clinicopathologic parameters and survival data. HPV DNA was detected by PCR in 150 of 193 (73.4%) tissue specimens of cervical cancer patients. DNA sequence analysis revealed the presence of HPV 16 (n = 68, 45.3%), HPV 18 (n = 49, 32.6%) and rare HPV types (n = 33, 22.1%). HPV genotypes correlated significantly with histologic tumor types, node status, tumor oxygenation, blood vessel invasion, and lymph space involvement. The presence of HPV DNA in cervical cancer as well as the genotype of HPV 16 could also be confirmed as significant prognostic factors in the univariate Cox regression analysis (RR 2.856, P < 0.003 resp. RR 3.444, P < 0.0001). In the multivariate analysis, however, HPV DNA status failed to be of prognostic relevance. Exclusively HPV 16 appears to have an independent impact on the overall survival in cervical patients (RR 3.653, P < 0.002). We conclude that the detection of HPV 16 genotype may play an important adjunct role in assessing prognosis of cervical cancer patients. The clinical impact of the presence of HPV DNA in primary tumors of uterine cervix remains to be investigated in further studies, and the exact mechanisms by which HPV influences the prognosis of cervical cancer patients have to be defined.

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“…TA B L E 2 HPV genotyping distribution in samples with multiple HPV types, in the studied population High-risk alpha-papillomavirus.The alpha-papillomavirus types16,33, 45, 51, 58, and 68 detected in our study are all associated with high-risk of malignant lesions,…”

mentioning

confidence: 51%

High frequency of multiple HPV types detection in Fanconi anemia patients oral swabs

Portugal

Raboni

Nogueira

et al. 2018

Transplant Infectious Dis

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Background Fanconi anemia (FA) is a rare genetic disease usually characterized by bone marrow failure and congenital malformations. The risk of development of malignancies in the oral cavity of FA patients, such as squamous cell carcinoma (SCC), increases significantly after a hematopoietic stem cells transplant (HSCT), and may also be linked with the presence of human papillomavirus (HPV) infections in the oral cavity. We investigated the prevalence and the HPV genotypes in oral mucosa of Brazilian FA patients. Methods and results Oral swabs of 49 FA patients were collected. The median age of patients was 20 years (range 5‐44) and 57% were over 18 years. Oral lesions were present in 20% of all patients, being 90% leukoplakia. HPV DNA was detected in 28% (14/49) of patients, and one of them also reported genital HPV lesions. Sixty‐seven percent of all patients had undergone HSCT, including 12 patients (86%) of those with HPV results. Multiple HPV types were detected in 78% and 71% of HPV samples by Sanger sequencing and reverse hybridization methods, respectively. The most prevalent HPV types detected were 6, 11, 18, and 68. Conclusions HPV prevalence in the oral mucosa of the assessed FA patients was higher than reported in the general population. Additional studies with collection of sequential samples are needed to know the natural history of the presence of multiple HPV types in these individuals and its association with the development of tumors, to evaluate the implementation of preventive measures, such as vaccination, and to guide early treatment.

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Intermethod variation in detection of human papillomavirus DNA in cervical smears

Cited by 56 publications

References 22 publications

Genital Human Papillomavirus Types in Immunocompetent and Immunodepressed Women in Northeast Italy: Prevalence and Cytomorphological Correlations

Genital Human Papillomavirus Types in Immunocompetent and Immunodepressed Women in Northeast Italy: Prevalence and Cytomorphological Correlations

The presence of HPV DNA in cervical cancer: Correlation with clinico-pathologic parameters and prognostic significance: 10 years experience at the Department of Obstetrics and Gynecology of the Mainz University

High frequency of multiple HPV types detection in Fanconi anemia patients oral swabs

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