Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways

Abstract

Objective. Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic differentiation capacity of SCAPs and elucidating the underlying molecular mechanisms. Materials and Methods. Here, SCAPs were isolated and identified in vitro. Effects of PTH on the proliferation of SCAPs were determined by Cell Counting Kit-8 (CCK-8), flow cytometry (FCM), and EdU. Alkaline phosphatase (ALP) activity, alizarin red staining, Western blot, and RT-PCR were carried out to detect the odonto/osteogenic differentiation of PTH-treated SCAPs as well as the participation of the MAPK signaling pathway. Results. An ALP activity assay determined that 10-8 mol/L PTH was the optimal concentration for the induction of SCAPs with no significant influence on the proliferation of SCAPs as indicated by CCK-8, FCM, and EdU. The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differentiation was suppressed following the inhibition of JNK and P38 MAPK pathways. Conclusion. PTH can regulate the odonto/osteogenic differentiation of SCAPs via JNK and P38 MAPK pathways.

Hormone and implant osseointegration: Elaboration of the relationship among function, preclinical, and clinical practice

“…Dental pulp stem cells (DPSCs) have been extensively studied in bone and tooth regenerations. It has been shown that the parathyroid hormone enhances the osteo/odontogenic differentiation of dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAPs) via the P38 MAPK pathways ( Ge et al, 2020 ; Pang et al, 2020 ). At the same time, more emphasis should be placed on the impact of PTH on the periodontal tissue ( Silva et al, 2016 ; Li et al, 2017 ).…”

EVL Promotes Osteo-/Odontogenic Differentiation of Dental Pulp Stem Cells via Activating JNK Signaling Pathway

“…Next, we sought to investigate the molecular mechanisms by which EVL regulated the differentiation of hDPSCs. It is well known that the MAPK signaling pathway regulates a variety of biological processes, including development, proliferation, differentiation, motility, survival, and apoptosis [51,52] .Some studies showed that MAPK signaling pathway played a crucial role in mediating osteo-/odontogenic differentiation and are likely regulated by EVL [38,39,[53][54][55]. In the present study, to investigate whether EVL enhanced osteo-/odontogenic differentiation through activating MAPK pathway, we examined the activation of key signaling molecules of the MAPK pathway, including p38 MAPKs, JNKs, and ERKs of MAPK pathway in cells transfected with pM-EVL, si-EVL, and negative control.…”

Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration

“…Mechanisms of DIM in regulating the promotion of BMMSC osteogenic activities needed to be explored. It is well-known that a number of signaling pathways contribute to osteogenic programs of MSCs, such as the mitogen-activated protein kinase (MAPK), Notch, and NF- κ B pathways [ 40 ]. MAPK superfamily, including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, is one of the most thoroughly studied signal transduction systems.…”