Intermittent fasting causes metabolic stress and leucopenia in young mice

Sorochynska, Oksana M.; Bayliak, Maria M.; Vasylyk, Yulia V.; Kuzniak, Oksana V.; Drohomyretska, Ivanna Z.; Klonovskyi, Andriy Ya.; Storey, Janet M.; Storey, Kenneth B.; Lushchak, Volodymyr I.

doi:10.15407/ubj91.01.053

Cited by 19 publications

(3 citation statements)

References 35 publications

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“…However, no differences in the levels of glucose, cholesterol, triacylglycerides, and activities of alkaline phosphatase (ALP) and LDH in plasma were found (Xie et al, 2017). In our previous work (Sorochynska et al, 2019), where we used the same experimental design as in the current study, blood parameters were similar to those described in the above cited work (Xie et al, 2017). In particular, we found lower leucocyte counts, circulating glucose and lactate levels, no difference in the activity of ALP, or in hemoglobin levels or erythrocyte count, whereas LDH activity was higher in males and lower in females of EODF group than that in AL groups (Sorochynska et al, 2019).…”

Section: Discussionmentioning

confidence: 96%

“…In our previous work (Sorochynska et al, 2019), where we used the same experimental design as in the current study, blood parameters were similar to those described in the above cited work (Xie et al, 2017). In particular, we found lower leucocyte counts, circulating glucose and lactate levels, no difference in the activity of ALP, or in hemoglobin levels or erythrocyte count, whereas LDH activity was higher in males and lower in females of EODF group than that in AL groups (Sorochynska et al, 2019). The difference between these two studies may be related to factors including: (1) mouse strain specificity, (2) different animal ages, or (3) experimental design.…”

Section: Discussionmentioning

confidence: 96%

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Every-Other-Day Feeding Decreases Glycolytic and Mitochondrial Energy-Producing Potentials in the Brain and Liver of Young Mice

et al. 2019

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Intermittent fasting is used to reduce body mass in obese adult humans and animals. However, information on the impact of one type of intermittent fasting (IF) called every-other-day feeding (EODF) on young animals is scarce. In this study, 1-month-old mice of both sexes were subjected to a 4-week regimen of EODF using age-matched counterparts fed ad libitum as controls. At the end of EODF exposure, experimental male and female mice weighed 14 and 13% less than the control counterparts. The EODF regimen resulted in lower liver levels of glycogen, glucose, and lactate, but did not affect lactate level in mouse cerebral cortex of both sexes. Activities of key glycolytic enzymes (hexokinase, phosphofructokinase, and pyruvate kinase) in liver of experimental mice were lower than those in controls. In the cerebral cortex, only hexokinase and pyruvate kinase activities were lower than in controls, but phosphofructokinase activity was not affected in IF females and was higher in IF males as compared with ad libitum fed males. Mitochondria isolated from liver of IF mice had lower respiratory control ratios, but those from the cortex had the same values as control animals. The concentration of β-hydroxybutyrate and the activity of β-hydroxybutyrate dehydrogenase were lower in the IF mouse liver, but not changed or enhanced in the IF cerebral cortex. Thus, animal responses to IF do not depend significantly on sex and are directed to decrease energy metabolism to save resources, and the effects are more pronounced in the liver than in the brain.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Every-Other-Day Feeding Decreases Glycolytic and Mitochondrial Energy-Producing Potentials in the Brain and Liver of Young Mice

et al. 2019

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“…The majority of rodent and human studies have generally demonstrated that dietary restriction (intermittent and short-term fasting) decreased plasma glucose and other parameters of carbohydrate metabolism, whereas fat oxidation was increased (Tinsley and La Bounty, 2015[ 47 ]; Antoni et al, 2017[ 3 ]; Sorochynska et al, 2019[ 45 ][ 46 ]; Freire et al, 2020[ 10 ]). Lipid oxidation, in turn, significantly stimulates ROCS formation, resulting in the development of carbonyl/oxidative stress (Niki, 2009[ 31 ]; Onyango, 2012[ 32 ]; Semchyshyn, 2014[ 39 ]; Garaschuk et al, 2018[ 11 ]).…”

Section: Discussionmentioning

confidence: 99%

Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice

Kuzniak

Sorochynska

Bayliak

et al. 2022

EXCLI Journal; 21:Doc77; ISSN 1611-2156

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Intermittent fasting as a dietary intervention can prevent overweight and obesity in adult organisms. Nevertheless, information regarding consequences of intermittent fasting for redox status and reactive metabolite-mediated processes that are crucial for the normal functioning of organisms is limited. Since the information on effects of intermittent fasting on parameters of oxidative/carbonyl stress in the brains of young mice was absent, the present study addressed these questions using an every-other-day fasting (EODF) protocol. The levels of carbonyl proteins were ~28 %, 22 % and 18 % lower in the cerebral cortex of EODF males and females and middle parts of the brain of EODF males, respectively, as compared to their ad libitum fed counterparts. Lipid peroxides and α-dicarbonyl compounds were lower only in the cortex and medulla part of EODF male brain. The EODF regimen resulted in higher total non-specific antioxidant capacity in different parts of male brain and a tendency to be higher this parameter in females. At the same time, EODF regimen had no effect on the activities of the defensive antioxidant enzymes, namely superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, glyoxylase 1 and glucose-6-phosphate dehydrogenase in the cortex of both sexes, but even decreased activities of these enzymes in medulla and middle part of the brain. In general, the results suggest that in the brain of young mice ad libitum feeding induces mild oxidative/carbonyl stress which may be partially alleviated by the EODF regimen. The effect of EODF regimen is more pronounced in the medulla part than in the cortex.

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Middle aged turn point in parameters of oxidative stress and glucose catabolism in mouse cerebellum during lifespan: minor effects of every-other-day fasting

et al. 2021

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Intermittent fasting causes metabolic stress and leucopenia in young mice

Cited by 19 publications

References 35 publications

Every-Other-Day Feeding Decreases Glycolytic and Mitochondrial Energy-Producing Potentials in the Brain and Liver of Young Mice

Every-Other-Day Feeding Decreases Glycolytic and Mitochondrial Energy-Producing Potentials in the Brain and Liver of Young Mice

Feeding to satiation induces mild oxidative/carbonyl stress in the brain of young mice

Middle aged turn point in parameters of oxidative stress and glucose catabolism in mouse cerebellum during lifespan: minor effects of every-other-day fasting

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