Intermittent High Glucose Stimulate MCP-l, IL-18, and PAI-1, but Inhibit Adiponectin Expression and Secretion in Adipocytes Dependent of ROS

Sun, Jiazhong; Xu, Yancheng; Dai, Zhijiang; Sun, Ying

doi:10.1007/s12013-009-9066-3

Cited by 48 publications

(50 citation statements)

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“…The mRNA for PAI-1, SERPINE1, produced by both macrophages and adipocytes and involved in the fibrinolytic system (43), was found in greater abundance in MetS omental WAT. These results echo previous studies showing increased circulating levels of PAI-1 in obese MetS subjects (23) and a role for PAI-1 in the development of insulin resistance (29) and may be partially explained by the finding that increased concentrations of glucose and free fatty acids increase PAI-1 expression (22,47). At the same time, gene expression of the M2 macrophage-associated marker arginase-2 (ARG2) (32,34) was decreased in the sc WAT of the MetS obese group.…”

Section: E242 Adipose Extracellular Matrix Phenotypes In Obesitysupporting

confidence: 89%

Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity

Lackey

Burk

Ali³

et al. 2014

American Journal of Physiology-Endocrinology and Metabolism

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plays an important role in the maintenance of white adipose tissue (WAT) architecture and function, and proper ECM remodeling is critical to support WAT malleability to accomodate changes in energy storage needs. Obesity and adipocyte hypertrophy place a strain on the ECM remodeling machinery, which may promote disordered ECM and altered tissue integrity and could promote proinflammatory and cell stress signals. To explore these questions, new methods were developed to quantify omental and subcutaneous WAT tensile strength and WAT collagen content by three-dimensional confocal imaging, using collagen VI knockout mice as a methods validation tool. These methods, combined with comprehensive measurement of WAT ECM proteolytic enzymes, transcript, and blood analyte analyses, were used to identify unique pathophenotypes of metabolic syndrome and type 2 diabetes mellitus in obese women, using multivariate statistical modeling and univariate comparisons with weightmatched healthy obese individuals. In addition to the expected differences in inflammation and glycemic control, approximately 20 ECMrelated factors, including omental tensile strength, collagen, and enzyme transcripts, helped discriminate metabolically compromised obesity. This is consistent with the hypothesis that WAT ECM physiology is intimately linked to metabolic health in obese humans, and the studies provide new tools to explore this relationship. matrix metalloproteinase; bariatric surgery; adipose inflammation; type 2 diabetes mellitus; extracellular matrix WHITE ADIPOSE TISSUE (WAT) is the primary site of energy storage in humans and by its nature must be malleable to respond to changes in energy balance through expansion and contraction. Under healthy conditions, changes in WAT architecture and the extracellular matrix (ECM) are coordinated with alterations in adipocyte size to accommodate fat storage needs. In contrast, sustained excessive energy intake with concomitant development of hypertrophic adipocytes creates a strain on ECM adaptation with pathophysiological consequences such as inflammation and insulin resistance.Adipose transcript abundances of several ECM-related genes were increased in obese compared with nonobese human subjects, including cell adhesion molecules and ECM receptor interaction genes, whereas weight loss after gastric bypass surgery led to decreased expression of these genes (18). Collagen VI is enriched in adipose tissue, and visceral WAT mass is related to increased expression of collagen 6␣3 in humans and in genetically obese mice (21, 38). Some studies have indicated that, at least in severe obesity, WAT fibrosis is more prevalent compared with nonobese individuals and that fibrosis decreases with weight loss (13,14), and this is believed to play a role in obesity-associated inflammation. Genetically obese ob/ob mice deficient in collagen VI (and thus lower fibrosis) fed a high-fat diet have improved glucose tolerance, insulin signaling, and triglyceride disposal with greater adipocyte size along with reduced WAT inf...

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Section: E242 Adipose Extracellular Matrix Phenotypes In Obesitysupporting

confidence: 89%

Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity

Lackey

Burk

Ali³

et al. 2014

American Journal of Physiology-Endocrinology and Metabolism

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“…Hypoadiponectinemia has been found to be associated with the obesity related pathological conditions such as metabolic syndrome (Liu and Liu 2009) that is considered as a condition of tissue infl ammation (Gustafson et al 2007). In addition to the presence of high plasma glucose, fat intake, and dyslipidemia with metabolic syndrome, are all known to inhibit adiponectin secretion in vitro (Oller do Nascimento et al 2009;Sun et al 2009;Lapointe et al 2011). …”

Section: Discussionmentioning

confidence: 99%

“…Over duration of three weeks, saliva samples were collected into test tubes between 9:00 a.m. and 12:00 noon, and kept in ice then centrifuged for 15 min at 1000 rpm at 4 °C. Th e supernatant was then divided into aliquots of 1 ml in Eppendorf tubes and stored at -80 °C till analysis (Th anakun et al 2013). Assay for the salivary adiponectin level.…”

Section: Methodsmentioning

confidence: 99%

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Salivary adiponectin concentration in healthy adult males in relation to anthropometric measures and fat distribution

Abdalla

Soon

2017

Endocrine Regulations

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Objectives. Body fat content, fat distribution, and adiponectin level are important variables in the development of obesity related complications. Anthropometric indices may provide an economic and faster method in measuring the risk for complications through their predictive eff ect of fat distribution and adiponectin concentration. We aimed to determine, which of the waist circumference (WC), waist-to-hip ratio (WHR), and body mass index (BMI) may be the best predictor for the total fat percentage (WF), visceral fat level (VF), and subcutaneous whole-body fat (SCWBF). We aimed also to investigate the potential use of the anthropometric measures and fat distribution as predictors for the salivary adiponectin level in the healthy adult males.Subjects. A total of 88 adult males aged between 18−25 years with a wide range of BMI were studied. Anthropometric indices were measured using standardized methods and salivary adiponectin level was assessed using an enzyme-linked immunosorbent assay.Results. In path analysis of the Structural Equation Model (SEM) using IBM@SPSS AMOS, version22, BMI and WC, but not WHR, were strong predictors for WF and SCWBF (p<0.05). BMI but not WC was a strong predictor for VF (p<0.001). WF was strong predictor for SCWBF (p<0.001), but not for VF. BMI, WC, WHR, WF, VF, and SCWBF were poor predictors of the salivary adiponectin level.Conclusion. BMI is the best predictor for the total body fat and fat distribution. However, WHR seems to be of a little value and the salivary adiponectin level independent of BMI and body fat in healthy adult Malay males.

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“…[15][16][17]71 Metabolic syndrome, because of its association with the infiltration of macrophages, is considered a state of tissue inflammation. 71 Conditions found in the metabolic syndrome such as high plasma glucose, 72 high dietary fat, 73 changes in very-low-density lipoprotein metabolism 74 and inflammatory cytokines 70,75 are all known to inhibit adiponectin secretion in vitro.…”

Section: Discussionmentioning

confidence: 99%

Lack of association between body mass index and plasma adiponectin levels in healthy adults

Kuo

2011

Int J Obes

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Objectives: To test the hypothesis that obesity (increase in fat mass) independently affects the level of adipokines: adiponectin, tumor necrosis factor-a (TNFa) and interleukin (IL)-6. Methods: Publications in the past decade reporting adult plasma adiponectin, leptin, TNFa and/or IL-6 levels were compiled. Mean gender-specific values were extracted from studies that included medical screening to confirm physical health (43 groups, total 4852 subjects). Correlation analysis was conducted between adipokine levels and body mass index (BMI), a widely used estimate of adiposity. Results: For healthy lean to obese adults of both genders, no significant correlation between plasma adiponectin and BMI was detected. There was also no gender difference in plasma adiponectin level. In contrast, leptin levels showed a positive correlation with BMI in both genders, and women had significantly higher levels of plasma leptin consistent with a higher percentage of body fat. The proinflammatory cytokine TNFa failed to show correlation with BMI. Although IL-6 showed a positive correlation with BMI in women, the obesity-related increase was very limited. Conclusions: Data analysis based on studies performed on healthy adults did not support the hypothesis that obesity independently affects the plasma level of adiponectin and TNFa. Reported obesity-related changes in plasma adipokine levels may be a consequence of obesity-related metabolic disorders. Future studies are especially needed to understand the homeostasis of adiponectin.

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Intermittent High Glucose Stimulate MCP-l, IL-18, and PAI-1, but Inhibit Adiponectin Expression and Secretion in Adipocytes Dependent of ROS

Cited by 48 publications

References 45 publications

Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity

Contributions of adipose tissue architectural and tensile properties toward defining healthy and unhealthy obesity

Salivary adiponectin concentration in healthy adult males in relation to anthropometric measures and fat distribution

Lack of association between body mass index and plasma adiponectin levels in healthy adults

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