Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Li, Tiancheng; Wang, Han; Lv, Chunxiao; Huang, Li; Zhang, Cheng; Zhou, Chenchen; Zou, Shujuan; Duan, Peipei

doi:10.1002/jcp.29994

Cited by 12 publications

(16 citation statements)

References 49 publications

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“…The establishment of a root repair model was consistent with our previous study [ 30 ]. Thirty-six 6-week-old male SD rats weighing approximately 200 ± 10 g were randomly selected for our study.…”

Section: Methodssupporting

confidence: 89%

“…The samples were fixed in 4% paraformaldehyde (PFA) for 24 h and then examined using a high-resolution (10 μm) Micro-CT50 system (Scanco Medical, Wangen-Bruttisellen, Switzerland). The detailed analysis of root resorption was consistent with our previous study [ 30 ]. The 3D model of the left maxillary first molar was reconstructed using Mimics 21.0 software to observe the absorption craters (Additional file 2 : Fig.…”

Section: Methodssupporting

confidence: 89%

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Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Chen

et al. 2022

Stem Cell Res Ther

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Background Orthodontic tooth movement inevitably induces cementum resorption, which is an urgent problem for orthodontists to confront. Human periodontal ligament stem cells (hPDLSCs) exert an important role in the orthodontic tooth movement and exhibit multidirectional differentiation ability in cementum regeneration. Connective tissue growth factor (CTGF) is an important extracellular matrix protein for bone homeostasis and cell differentiation. The purpose of our study was to explore the role of CTGF in cementum repair and cementogenesis and to elucidate its underlying mechanism. Methods A cementum defect model was established by tooth movement with heavy forces, and the cementum repair effect of CTGF was observed via micro-CT, HE staining and immunohistochemical staining. RT‒qPCR, western blotting (WB), alizarin red staining and ALP activity experiments verified the mineralization ability of hPDLSCs stimulated with CTGF. The expression of Cx43 in periodontal ligament cells was detected by WB and immunofluorescence (IF) experiments after CTGF stimulation in vivo and in vitro. Subsequently, the mineralization ability of hPDLSCs was observed after application of CTGF and the small interfering RNA Si-Cx43. Additionally, co-intervention via application of the small interfering RNA Si-CTGF and the Cx43 agonist ATRA in hPDLSCs was performed to deepen the mechanistic study. Next, WB, IF experiments and co-immunoprecipitation were conducted to confirm whether CTGF triggers the Cx43/β-catenin axis to regulate cementoblast differentiation of hPDLSCs. Results Local oral administration of CTGF to the cementum defects in vivo facilitated cementum repair. CTGF facilitated the cementogenesis of hPDLSCs in a concentration-dependent manner. Cx43 acted as a downstream effector of CTGF to regulate cementoblast differentiation. Si-Cx43 reduced CTGF-induced cementoblast differentiation. The Cx43 agonist ATRA restored the low differentiation capacity induced by Si-CTGF. Further mechanistic studies showed that CTGF triggered the activation of β-catenin in a dose-dependent manner. In addition, co-localization IF analysis and co-immunoprecipitation demonstrated that Cx43 interacted with β-catenin at cell‒cell connections. Si-Cx43 attenuated the substantial expression of β-catenin induced by CTGF. The Cx43 agonist reversed the inhibition of β-catenin induced by Si-CTGF. IF demonstrated that the nuclear importation of β-catenin was related to the immense expression of Cx43 at cell‒cell junctions. Conclusions Taken together, these data demonstrate that CTGF promotes cementum repair and cementogenesis through activation of the Cx43/β-catenin signalling axis.

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Section: Methodssupporting

confidence: 89%

Section: Methodssupporting

confidence: 89%

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Chen

et al. 2022

Stem Cell Res Ther

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“…Currently, a number of other factors have been described that belong to the complex signal and effector network of cementoblast mineralization. In the literature, there are such diverse cellular factors as BMP2, BSP, TGFβ, and OPG [ 61 , 62 , 63 ], which may also be involved in or influence the CAP-induced process of mineralization.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Eggers

Marciniak

Deschner

et al. 2021

IJMS

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The aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (BGLAP), periostin (POSTN), osteopontin (OPN), osterix (OSX), collagen type I alpha 1 chain (COL1A1), dentin matrix acidic phosphoprotein (DMP)1, RUNX family transcription factor (RUNX)2, and marker of proliferation Ki-67 (KI67) was quantified by real-time PCR. Protein expression was analyzed by immunocytochemistry and ELISA. ALP activity was determined by ALP assay. Von Kossa and alizarin red staining were used to display mineralization. Cell viability was analyzed by XTT assay, and morphological characterization was performed by DAPI/phalloidin staining. Cell migration was quantified with an established scratch assay. CAP and EMD upregulated both mRNA and protein synthesis of ALP, POSTN, and OPN. Additionally, DMP1 and COL1A1 were upregulated at both gene and protein levels. In addition to upregulated RUNX2 mRNA levels, treated cells mineralized more intensively. Moreover, CAP treatment resulted in an upregulation of KI67, higher cell viability, and improved cell migration. Our study shows that CAP appears to have stimulatory effects on regeneration-associated cell functions in cementoblasts.

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“…Immunohistochemical staining was performed as described previously ( Li et al, 2021 ). Sections were incubated with primary antibodies diluted in blocking solution with different optimized dilution rates: OPG (dilution 1:100; R1608-4), Runt-related transcription factor 2 (RUNX2) (dilution 1:100; ET1612-47), Osterix (OSX) (dilution 1:400; ER1914-47), collagen type 1 (COL1) (dilution 1:100; ET1609-68), and alkaline phosphatase (ALP) (dilution 1:100; ET1601-21) from Huabio (Hangzhou, China); RANKL (dilution 1:200, ab169966) from Abcam (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

et al. 2021

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Orthodontic tooth movement (OTM) is a process depending on the remodeling of periodontal tissues surrounding the roots. Orthodontic forces trigger the conversion of mechanical stimuli into intercellular chemical signals within periodontal ligament (PDL) cells, activating alveolar bone remodeling, and thereby, initiating OTM. Recently, the mechanosensitive ion channel Piezo1 has been found to play pivotal roles in the different types of human cells by transforming external physical stimuli into intercellular chemical signals. However, the function of Piezo1 during the mechanotransduction process of PDL cells has rarely been reported. Herein, we established a rat OTM model to study the potential role of Piezo1 during the mechanotransduction process of PDL cells and investigate its effects on the tension side of alveolar bone remodeling. A total of 60 male Sprague-Dawley rats were randomly assigned into three groups: the OTM + inhibitor (INH) group, the OTM group, and the control (CON) group. Nickel-titanium orthodontic springs were applied to trigger tooth movement. Mice were sacrificed on days 0, 3, 7, and 14 after orthodontic movement for the radiographic, histological, immunohistochemical, and molecular biological analyses. Our results revealed that the Piezo1 channel was activated by orthodontic force and mainly expressed in the PDL cells during the whole tooth movement period. The activation of the Piezo1 channel was essential for maintaining the rate of orthodontic tooth movement and facilitation of new alveolar bone formation on the tension side. Reduced osteogenesis-associated transcription factors such as Runt-related transcription factor 2 (RUNX2), Osterix (OSX), and receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio were examined when the function of Piezo1 was inhibited. In summary, Piezo1 plays a critical role in mediating both the osteogenesis and osteoclastic activities on the tension side during OTM.

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Intermittent parathyroid hormone promotes cementogenesis via ephrinB2‐EPHB4 forward signaling

Cited by 12 publications

References 49 publications

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro

Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement

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