Intermolecular tuning of calmodulin by target peptides and proteins: Differential effects on Ca²⁺ binding and implications for kinase activation

Abstract

Ca2+-activated calmodulin (CaM) regulates many target enzymes by docking to an amphiphilic target helix of variable sequence. This study compares the equilibrium Ca2+ binding and Ca2+ dissociation kinetics of CaM complexed to target peptides derived from five different CaM-regulated proteins: phosphorylase kinase, CaM-dependent protein kinase 11, skeletal and smooth myosin light chain kinases, and the plasma membrane Ca2+-ATPase. The results reveal that different target peptides can tune the Ca2+ binding affinities and kinetics of the two CaM domains over a wide range of Ca2+ concentrations and time scales. The five peptides increase the Ca2+ affinity of the N-terminal regulatory domain from 14-to 350-fold and slow its Ca2+ dissociation kinetics from 60-to 140-fold. Smaller effects are observed for the C-terminal domain, where peptides increase the apparent Ca2+ affinity 8-to 100-fold and slow dissociation kinetics 13-to 32-fold. In full-length skeletal myosin light chain kinase the inter-molecular tuning provided by the isolated target peptide is further modulated by other tuning interactions, resulting in a CaM-protein complex that has a 10-fold lower Ca2+ affinity than the analogous CaM-peptide complex. Unlike the CaM-peptide complexes, Ca2+ dissociation from the protein complex follows monoexponential kinetics in which all four Ca2+ ions dissociate at a rate comparable to the slow rate observed in the peptide complex. The two Ca2+ ions bound to the CaM N-terminal domain are substantially occluded in the CaM-protein complex. Overall, the results indicate that the cellular activation of myosin light chain kinase is likely to be triggered by the binding of free CaZ2+-CaM or Cq2+-CaM after a Ca2+ signal has begun and that inactivation of the complex is initiated by a single rate-limiting event, which is proposed to be either the direct dissociation of Ca2+ ions from the bound C-terminal domain or the dissociation of Ca2+ loaded C-terminal domain from skMLCK. The observed target-induced variations in Ca2+ affinities and dissociation rates could serve to tune CaM activation and inactivation for different cellular pathways, and also must counterbalance the variable energetic costs of driving the activating conformational change in different target enzymes.Keywords: calcium signaling; calmodulin; kinase regulation; protein-protein interactionsThe ubiquitous protein calmodulin (CaM) plays a crucial role in Ca2+ signaling (Beckingham, 1995;Braun & Schulman, 1995; Clapham, 1995;Wolenski, 1995; Berridge, 1996), where it regulates a wide range of cellular processes by serving as an intracellular receptor for Ca2+ ions (Wheeler et al

Conserved properties of individual Ca 2+ -binding sites in calmodulin

“…Adding to the complexity, a single target may bind CaM with different conformations and stoichiometries that are Ca 2+ -dependent (67). Protein targets of CaM affect the apparent Ca 2+ affinity (68,69), and the protein complex is tuned to diverse physiological roles through evolution (70)(71)(72). How do the four EF-loops contribute to these diverse roles?…”

Calmodulin Transduces Ca2+ Oscillations into Differential Regulation of Its Target Proteins

“…CaM contains four EF-hands, each binding one Ca 2+ ion, that are organized as pairs in two globular domains connected by a flexible tether ( Figure 1). 29,30 Pairing of the EF-hands allows each domain to bind two Ca 2+ ions with positive cooperativity; 31,32 thus, species with zero, two, or four Ca 2+ ions bound dominate over those with one or three Ca…”

Differential Integration of Ca2+-Calmodulin Signal in Intact Ventricular Myocytes at Low and High Affinity Ca2+-Calmodulin Targets

“…Simulations in which Ca 2ϩ dissociation was blocked produced greatly slowed inactivation, consistent with this interpretation. Ca 2ϩ dissociation has been previously shown to be a significant inactivation pathway in experiments and models of purified CaM targets including MLCK (38,39), CaMKII (40), and CaN (41).…”