Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant

Mujib, A.; Bansal, Yashika; Malik, Moien Qadir; Syeed, Rukaya; Mamgain, Jyoti; Ejaz, Bushra

doi:10.1007/978-1-0716-2485-2_2

Cited by 5 publications

(3 citation statements)

References 86 publications

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“…The successful conversion of somatic embryos into healthy plantlets was achieved more efficiently in MS fortified with varied levels of BAP as compared to PGR-free MS medium. Similar somatic embryo germination responses were recorded in PGR-added media in various plant species [ 52 , 53 ]. Somatic embryo germination is, however, frequently regulated by a number of factors including plant genotype, PGRs, culture conditions, photoperiod, and in interaction with endogenous levels of hormones [ 54 ].…”

Section: Discussionsupporting

confidence: 66%

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Bansal

Mujib

Siddiqui

et al. 2022

Genes

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Digitalis purpurea L. is a therapeutically important plant that synthesizes important cardiotonics such as digitoxin and digoxin. The present work reports a detailed and efficient propagation protocol for D. purpurea by optimizing various PGR concentrations in Murashige and Skoog (MS) medium. The genetic homogeneity of in vitro regenerants was assessed by the flow cytometric method (FCM) and Start Codon Targeted (SCoT) marker technique. Firstly, the seeds inoculated in full MS medium added with 0.5 mg/L GA3 produced seedlings. Different parts such as hypocotyl, nodes, leaves and apical shoots were used as explants. The compact calli were obtained on BAP alone or in combinations with 2, 4-D/NAA. The hypocotyl-derived callus induced somatic embryos which proliferated and germinated best in 0.75 mg/L BAP-fortified MS medium. Scanning electron microscopic (SEM) images confirmed the presence of various developmental stages of somatic embryos. Shoot regeneration was obtained in which BAP at 1.0 mg/L and 2.0 mg/L BAP + 0.5 mg/L 2,4-D proved to be the best treatments of PGRs in inducing direct and indirect shoot buds. The regenerated shoots showed the highest rooting percentage (87.5%) with 24.7 ± 1.9 numbers of roots/shoot in 1.0 mg/L IBA augmented medium. The rooted plantlets were acclimatized in a greenhouse at a survival rate of 85–90%. The genome size and the 2C nuclear DNA content of field-grown, somatic embryo-regenerated and organogenic-derived plants were estimated and noted to be 3.1, 3.2 and 3.0 picogram (pg), respectively; there is no alteration in ploidy status and the DNA content, validating genetic uniformity. Six SCoT primers unveiled 94.3%–95.13% monomorphic bands across all the plant samples analyzed, further indicating genetic stability among in vitro clones and mother plants. This study describes for the first time successful induction of somatic embryos from hypocotyl callus; and flow cytometry and SCoT marker confirmed the genetic homogeneity of regenerated plants.

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Section: Discussionsupporting

confidence: 66%

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Bansal

Mujib

Siddiqui

et al. 2022

Genes

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“…Plant tissue culture proves to be an effective biotechnological tool for the rapid propagation of plants under aseptic conditions with a lesser risk of microbial infections [3]. Several in vitro studies using different explants have been successfully conducted for somatic embryogenesis [4] and organogenesis in C. roseus [5,6].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition and Detection of Novel Bioactives in Anther Callus of Catharanthus roseus L.

Bansal

Mujib

Mamgain

et al. 2023

Plants

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Catharanthus roseus L. (G.) Don is the most widely studied plant because of its high pharmacological value. In vitro culture uses various plant parts such as leaves, nodes, internodes and roots for inducing callus and subsequent plant regeneration in C. roseus. However, till now, little work has been conducted on anther tissue using plant tissue culture techniques. Therefore, the aim of this work is to establish a protocol for in vitro induction of callus by utilizing anthers as explants in MS (Murashige and Skoog) medium fortified with different concentrations and combinations of PGRs. The best callusing medium contains high α-naphthalene acetic acid (NAA) and low kinetin (Kn) concentrations showing a callusing frequency of 86.6%. SEM–EDX analysis was carried out to compare the elemental distribution on the surfaces of anther and anther-derived calli, and the two were noted to be nearly identical in their elemental composition. Gas chromatography–mass spectrometry (GC–MS) analysis of methanol extracts of anther and anther-derived calli was conducted, which revealed the presence of a wide range of phytocompounds. Some of them are ajmalicine, vindolinine, coronaridine, squalene, pleiocarpamine, stigmasterol, etc. More importantly, about 17 compounds are exclusively present in anther-derived callus (not in anther) of Catharanthus. The ploidy status of anther-derived callus was examined via flow cytometry (FCM), and it was estimated to be 0.76 pg, showing the haploid nature of callus. The present work therefore represents an efficient way to produce high-value medicinal compounds from anther callus in a lesser period of time on a larger scale.

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“…DRs have been extensively researched to enhance transformation efficiency and induce meristematic or embryogenic division [ 15 , 16 , 17 , 18 ]. Of the many DRs that help realize plasticity and totipotency, SOMATIC EMBRYOGENESIS LIKE RECEPTOR KINASE (SERK), LEAFY COTYLEDON (LEC), AGAMOUS-LIKE 15 (AGL15), Wuschel (WUS), and BABY BOOM (BBM) are known to be crucial [ 19 ]. More specifically, regeneration frequency and transformation efficiency were reported to be enhanced by Baby Boom (BBM) and Wuschel2 (WUS) in both dicot and monocot plants [ 10 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

BabyBoom: 3-Dimensional Structure-Based Ligand and Protein Interaction Prediction by Molecular Docking

Panchangam¹

2022

Biomolecules

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Baby Boom (BBM) is a key transcription factor that triggers embryogenesis, enhances transformation and regeneration efficiencies, and regulates developmental pathways in plants. Triggering or activating BBM in non-model crops could overcome the bottlenecks in plant breeding. Understanding BBM’s structure is critical for functional characterization and determination of interacting partners and/or ligands. The current in silico study aimed to study BBM’s sequence and conservation across all plant proteomes, predict protein-protein and protein-ligand interactions, and perform molecular docking and molecular dynamics (MD) simulation to specifically determine the binding site amino acid residues. In addition, peptide sequences that interact with BBM have also been predicted, which provide avenues for altered functional interactions and the design of peptide mimetics that can be experimentally validated for their role in tissue culture or transformation media. This novel data could pave the way for the exploitation of BBM’s potential as the master regulator of specialized plant processes such as apomixes, haploid embryogenesis, and CRISPR/Cas9 transgenic development.

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Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant

Cited by 5 publications

References 86 publications

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Ploidy Status, Nuclear DNA Content and Start Codon Targeted (SCoT) Genetic Homogeneity Assessment in Digitalis purpurea L., Regenerated In Vitro

Phytochemical Composition and Detection of Novel Bioactives in Anther Callus of Catharanthus roseus L.

BabyBoom: 3-Dimensional Structure-Based Ligand and Protein Interaction Prediction by Molecular Docking

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