2015
DOI: 10.1007/s13361-015-1081-6
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Internal Energy Deposition for Low Energy, Femtosecond Laser Vaporization and Nanospray Post-ionization Mass Spectrometry using Thermometer Ions

Abstract: Abstract. The internal energy of p-substituted benzylpyridinium ions after laser vaporization using low energy, femtosecond duration laser pulses of wavelengths 800 and 1042 nm was determined using the survival yield method. Laser vaporization of dried benzylpyridinium ions from metal slides into a buffered nanospray with 75 μJ, 800 nm laser pulses resulted in a higher extent of fragmentation than conventional nanospray due to the presence of a two-photon resonance fragmentation pathway. Using higher energy 80… Show more

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Cited by 8 publications
(9 citation statements)
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“…58 Internal energy measurements of dried and liquid thermometer ions using the 1042 nm fiber laser show that F-LEMS is as soft as nanospray with little fragmentation and equivalent mean internal energy distributions. 59 This again suggests that fiber-based femtosecond laser vaporization is a "soft" process even with a burst of 20 successive pulses with a pulse intensity of 10 12 W/cm 2 . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 19…”
Section: Vaporization Of Aqueous Cytochrome C Film From Glass Slides mentioning
confidence: 97%
“…58 Internal energy measurements of dried and liquid thermometer ions using the 1042 nm fiber laser show that F-LEMS is as soft as nanospray with little fragmentation and equivalent mean internal energy distributions. 59 This again suggests that fiber-based femtosecond laser vaporization is a "soft" process even with a burst of 20 successive pulses with a pulse intensity of 10 12 W/cm 2 . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 19…”
Section: Vaporization Of Aqueous Cytochrome C Film From Glass Slides mentioning
confidence: 97%
“…The laser vaporization source was described in detail previously for the Ti:Sapphire-LEMS [29] and has been recently modified to couple the fiber laser to the electrospray inlet of a quadrupole time of flight (QTOF) mass spectrometer (micrOTOF-Q II; Bruker Daltonik GmbH, Bremen, Germany) [35,36]. Briefly, the fiber laser delivers 100 kHz, 435 fs, 1042 nm pulses with maximum pulse energy of 50 μJ.…”
Section: Laser Electrospray Mass Spectrometrymentioning
confidence: 99%
“…However, the higher cost and lower robustness of the Ti:Sapphire amplified femtosecond laser in comparison with the more common nanosecond UV and IR lasers limit the wide application of Ti:Sapphire laser as a desorption source. In recent studies, we successfully implemented a low-energy, femtosecond fiber laser for vaporization and a nanospray for postionization in the analysis of proteins, whole blood [35], and substituted benzylpyridinium salts [36]. The studies show that the low-energy fiber laser resulted in comparable internal energy distributions to nanospray [36] and retained intact biological macromolecules after laser radiation [35].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Unlike nanosecond laser pulses where a resonance must occur in a specific matrix, nonresonant, multiphoton laser vaporization occurs in LEMS without the aid of a matrix. Studies with thermometer ions and peptides show that LEMS produces ions with internal energies comparable to those produced by conventional ESI [35,36]. In the LEMS experiment, samples in numerous states (liquid, dried, mixtures, tissue, etc.)…”
Section: Introductionmentioning
confidence: 99%