Background A surgical site infection is a substantial cause of complications in patients. Different methods are being used to decrease surgical site infections; however, these infections still can cause complications, especially in patients undergoing longer operations ([ 3 hours). There is evidence that the efficacy of the scrubbing material fades after 3 hours. However, we do not know the longevity of hand cleanliness after application of scrubbing materials in a long operation. It can be postulated that if the surgeon's scrubbed hands are recolonized after a certain time, they may serve as a progressive source of contamination during surgery. Questions/purposes We asked: (1) Is there a correlation between surgical duration and hand contamination at the end of surgery? (2) At what point during surgery does hand contamination reach or exceed prescrub levels? Methods Three spine surgeons using the same scrubbing technique and materials consisting of chlorhexidine gluconate 1% solution and ethyl alcohol 61% w/w were enrolled in our study. Between December 2014 and April 2015, spine procedures of 3 hours or more, which were the One of the authors (PH) received funding from The San Diego Spine Foundation for this study. One of the authors certifies that he (BAA) or a member of his immediate family, has or may receive payments or benefits, during the study period, an amount of USD 10,000 to 100,000 from Nuvasive (San Diego, CA, USA), K2M (Leesburg, VA, USA), and DePuy Synthes (West Chester, PA, USA), and Ellipse (Aliso Viejo, CA, USA); an amount of USD 100,000 to 1000,000 from Nuvasive (San Diego, CA, USA); an amount of less than USD 10,000 from Ellipse (Aliso Viejo, CA, USA), Kspine (Leesburg, VA, USA), International Spine Study Group (Brighton, CO, USA), Nuvasive (San Diego, CA, USA), and K2M (Leesburg, VA, USA); Growing Spine Foundation (Milwaukee, WI, USA), San Diego Spine Foundation (San Diego, CA, USA), Scoliosis Research Society (Milwaukee, WI, USA), and Society of Lateral Access Surgery (San Diego, CA, USA). One of the authors certifies that he (GMM) or a member of his immediate family, has or may receive payments or benefits, during the study period, an amount of USD 100,000 to 1,000,000 from Nuvasive (San Diego, CA, USA); an amount of less than USD 10,000 from K2M (Leesburg, VA, USA), Medicrea (Lyon, France), Misonix (Farmingdale, NY, USA), International Spine Study Group Foundation (Brighton, CO, USA), Society of Lateral Access Surgery (San Diego, CA, USA), Scoliosis Research Society (Milwaukee, WI, USA), and San Diego Spine Foundation (San Diego, CA, USA). One of the authors certifies that he (RE) or a member of his immediate family, has or may receive payments or benefits, during the study period, an amount of less than USD10,000 from Globus Medical (Audubon, PA, USA), Aesculap/B. Braun (Center Valley, PA, USA), DePuy (West Chester, PA, USA), Johnson & Johnson Company (New Brunswick, NJ, USA), Eli Lilly (Indianapolis, IN, USA), Synthes (West Chester, PA, USA), DiFusion (Georgetown, TX, USA), Inv...