Internal standard response variations during incurred sample analysis by LC–MS/MS: Case by case trouble-shooting

Tan, Aimin; Hussain, Saleh; Musuku, Adrien; Massé, Robert

doi:10.1016/j.jchromb.2009.08.019

Cited by 70 publications

(47 citation statements)

References 12 publications

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“…Apart from automation, minimization of matrix effects and other variability sources is crucial to improve the overall method. The use of stable isotopic labeled internal standards (SIL-ISs) [23][24][25] with physico-chemical properties identical or similar to the target analytes is critical to overcome variability sources [26]. Proficiency testing programs have been created to assess comparative performances and improve the accuracy of total 25(OH)D and 1,25(OH) 2 D methods [3,27].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analytical method to evaluate the metabolism of vitamin D

Mena-Bravo

Ferreiro‐Vera²,

Priego-Capote

et al. 2015

Clinica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Quantitative analytical method to evaluate the metabolism of vitamin D

Mena-Bravo

Ferreiro‐Vera²,

Priego-Capote

et al. 2015

Clinica Chimica Acta

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“…Taking into account that an IS com-pound is usually added by a repeater pipette, small volumes (50 µL or less) are more prone to imprecision than large ones. Therefore, higher volumes are suggested [23]. Specifically, for this study, 100 µL (10% of total volume) was considered as appropriate to be mixed with 900 µL of samples or standards [20].…”

Section: Calibration Studymentioning

confidence: 99%

Harmonization of the quantitative determination of volatile fatty acids profile in aqueous matrix samples by direct injection using gas chromatography and high-performance liquid chromatography techniques: Multi-laboratory validation study

Raposo

Borja

Cacho

et al. 2015

Journal of Chromatography A

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A b s t r a c tThe performance parameters of volatile fatty acids (VFAs) measurements were assessed for the first time by a multi-laboratory validation study among 13 laboratories. Two chromatographic techniques (GC and HPLC) and two quantification methods such as external and internal standard (ESTD/ISTD) were combined in three different methodologies GC/ESTD, HPLC/ESTD and GC/ISTD. Linearity evaluation of the calibration functions in a wide concentration range (10-1000 mg/L) was carried out using different statistical parameters for the goodness of fit.Both chromatographic techniques were considered similarly accurate. The use of GC/ISTD, despite showing similar analytical performance to the other methodologies, can be considered useful for the harmonization of VFAs analytical methodology taking into account the normalization of slope values used for the calculation of VFAs concentrations. Acceptance criteria for VFAs performance parameters of the multi-laboratory validation study should be established as follows: (1) instrument precision (RSDINST≤ 1.5%); (2) linearity (R 2 ≥ 0.998; RSDSENSITIVITY≤ 4%; REMAX≤ 8%; REAVER≤3%); (3) precision (RSD ≤ 1.5%); (4) trueness (recovery of 97-103%);(5) LOD (≤3 mg/L); and (6) LOQ (10 mg/L).

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“…fortifying control (blank) with different amounts of analyte. While this approach is generally effective, it is not uncommon that bioanalytical issues arise upon applying a validated method to the analysis of incurred (study) samples, which are usually quite different from the spiked samples [1]. Such differences include the absence of various phase I or phase II metabolites and formulation-related components in the spiked samples.…”

Section: Introductionmentioning

confidence: 99%