Internal validation of the 7500 Real Time PCR System for use in forensic casework in Hellenic Police

Prenatal samples used as DNA evidence in rape cases

“…DNA extraction from blood samples, DNA quantification, PCR and electrophoresis were performed using the standard protocols employed in our laboratory [5].…”

“…Nevertheless, their usefulness in both deficiency paternity cases as well as in forensic genetic cases, where the analysis of autosomal STRs failed, is well recognized [2,3]. Particularly, in many mixed stains where the minor component is attributed to a male person, the minor's component profile can be detected only through Y-STR analysis [5,6]. Y-STR typing of the two samples produced a common Y-STR profile (Fig.…”