Internalization of amelogenin by dendritic cells of the papillary layer during transition and early maturation stages

Nishikawa, Sumio; Sasaki, Fumio

doi:10.1007/s004180050451

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Some of the specimens were developed with the AEC substrate kit (DAKO; Carpentia, CA). The sections were further labeled with 1 g/ml Hoechst 33342 (Molecular Probes; Eugene, OR) to detect the transition zone (Nishikawa and Sasaki 1999a) or rhodamine-phalloidin (Molecular Probes) diluted 1:20 with PBS to detect ruffle-ended (RA) or smoothended ameloblasts (SA) (Nishikawa and Josephsen 1987). Procedures for pre-embedding immunoelectron microscopy were described elsewhere (Nishikawa and Sasaki 1999b).…”

Section: Methodsmentioning

confidence: 99%

Localization of Transcription Factor AP-1 Family Proteins in Ameloblast Nuclei of the Rat Incisor

Nishikawa

2000

J Histochem Cytochem.

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We examined by immunocytochemistry the localization of the AP-1 family proteins c-Jun, JunB, JunD, c-Fos, FosB, Fra-1, and Fra-2 in rat incisor ameloblasts. Most of the antibodies against AP-1 family proteins, except for c-Fos-specific antibody, labeled ameloblast nuclei. The labeling intensity of the c-Jun, JunD, and Fra-2 antibodies was stronger than that of JunB, FosB, and Fra-1. Antibody reactivities of c-Jun, JunD, and Fra-2 were greatly enhanced during or after the transition zone. Furthermore, c-Jun antibodies labeled maturation ameloblasts in a cyclic pattern, which was correlated with ameloblast modulation. Disruption of ameloblast modulation by colchicine injection resulted in greatly decreased reactivity of the c-Jun antibody in the ameloblast nuclei of the maturation zone. Phospho-specific antibodies to c-Jun labeled ameloblast nuclei only weakly throughout the secretion, transition, and maturation zones. These results suggest that the stage-specific localization of AP-1 in ameloblasts is closely related to tooth enamel formation.

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Section: Methodsmentioning

confidence: 99%

Localization of Transcription Factor AP-1 Family Proteins in Ameloblast Nuclei of the Rat Incisor

Nishikawa

2000

J Histochem Cytochem.

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“…Proteolytical ECM remodeling not only removes ECM components, but also provides a pro-migratory microenvironment for living cells. In this sense, MMPs play a pivotal role during cell migration (Shapiro 1998), eliciting dendritic cells to phagocyte apoptotic cell fragments and anti-amelogenin-positive material (Nishikawa and Sasaki 1999). We showed that level of immunostaining for MMP-2 and -9 in macrophages and/or in dendritic like cells were highest during the transition phase, decreasing gradually throughout the maturation phase.…”

Section: Discussionmentioning

confidence: 94%

Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance

et al. 2009

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Reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is a single membrane-anchored MMP-regulator and regulates matrix metalloproteinases (MMP) 2, 9 and 14. In turn, MMPs are endopeptidases that play a pivotal role in remodeling ECM. In this work, we decided to evaluate expression pattern of RECK in growing rat incisor during, specifically focusing out amelogenesis process. Based on different kinds of ameloblasts, our results showed that RECK expression was conducted by secretory and post-secretory ameloblasts. At the secretory phase, RECK was localized in the infra-nuclear region of the ameloblast, outer epithelium, near blood vessels, and in the stellate reticulum. From the transition to the maturation phases, RECK was strongly expressed by non-epithelial immuno-competent cells (macrophages and/or dendritic-like cells) in the papillary layer. From the transition to the maturation stage, RECK expression was increased. RECK mRNA was amplified by RT-PCR from whole enamel organ. Here, we verified the presence of RECK mRNA during all stages of amelogenesis. These events were governed by ameloblasts and by non-epithelial cells residents in the enamel organ. Concluding, we found differential expression of MMPs-2, -9 and RECK in the different phases of amelogenesis, suggesting that the tissue remodeling is rigorously controlled during dental mineralization.

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“…It is interesting that dendritic cells beneath the ameloblast layer showed OX6‐immunoreactivity in the developing rat molars. Previous immunohistochemical studies have shown the existence of immunocompetent cells including OX6‐positive cells in the papillary layer during transition and maturation stages of amelogenesis in the rat incisor, a continuously growing tooth (29–31), and suggested the involvement of the class II MHC‐positive cells in the elimination of both enamel proteins such as amelogenin from the enamel organ and apoptotic cell debris (29,31). However, these data were obtained from the rat incisor, not molars.…”

Section: Discussionmentioning

confidence: 99%

Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat molars

Tamura

Nakakura-Ohshima

Maeda

et al. 2003

J of Periodontal Research

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The distribution of immunocompetent cells in the dentogingival junction of rat molars during root formation was investigated by immunocytochemistry using antibodies to class II major histocompatibility complex (MHC) molecules (OX6-antibody) and monocyte/macrophage lineage cells (ED1-antibody) as well as by histochemical reaction for periodic acid-Schiff (PAS). Two portions (the junctional epithelium in the mesial gingiva of the first molar, and the interdental gingiva between the first and second molars) were selected for observations. At the eruption stage of the first molar (16-18 days after birth), OX6-positive cells, dendritic or oval in shape, were abundantly distributed in the connective tissue between the oral epithelium and tooth germ. Positive cells with slender cell processes were also found beneath the ameloblast layer. At the commencement stage of the first molar occlusion (24-28 days after birth), numerous OX6-positive cells displaying a dendritic fashion existed preferentially in the mesial gingiva, but were fewer in the interdental gingiva. In contrast, the interdental gingiva showed a denser distribution of ED1-positive cells and PAS-reactive polymorphonuclear leukocytes (PMLs) than the mesial gingiva. At the completion stage of root formation (100-120 days after birth), the OX6-immunopositive cells invaded the deeper position of the mesial gingiva with the downgrowth of the epithelium; they had a considerably higher cell density compared with those in the interdental gingiva where PAS-reactive PMLs persisted. These findings indicated that the immunocompetent cells showed a region-specific distribution and cell density by their roles in immune response.

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Internalization of amelogenin by dendritic cells of the papillary layer during transition and early maturation stages

Cited by 7 publications

References 25 publications

Localization of Transcription Factor AP-1 Family Proteins in Ameloblast Nuclei of the Rat Incisor

Localization of Transcription Factor AP-1 Family Proteins in Ameloblast Nuclei of the Rat Incisor

Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance

Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat molars

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