International Association for Dental Research

Erikson, B.Edwin

doi:10.1177/00220345470260041001

Cited by 1 publication

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“…Considerable evidence exists indicating that during aerial mycelium development streptomycetes are able to synthesize hydrophobic sheath layers. These layers have been variously described as being composed of lipid (Erikson, 1947 ; Kalakoutskii, 1961), chitin (Smucker & Pfister, 1978), divalent cations (Pozharitskaja et al, 1974(Pozharitskaja et al, ,1976 or protein (Guijarro et al, 1988;Willey et al, 1991). There has been considerable interest in the functional role of hydrophobic cell wall proteins (hydrophobins) which appear to be involved in the formation of aerial growth in fungi (Wessels, 1997).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Streptomyces coelicolor A3(2) under nitrate-limited conditions

Karandikar

Sharples

Hobbs³

1997

Microbiology

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The life cycle of Streptomyces coelicolor during development on solid medium has been studied from a physiological perspective. A biphasic growth pattern was demonstrated, evidenced by a continuous transition from an initial exponential growth period into a slower phase of biomass accretion. The switch between the two phases coincided with the exhaustion of nitrate from the medium. The depletion of nitrate from the medium coincided with the initiation of aerial mycelium formation within the cultures and the development of hydrophobic surface properties. During secondary growth, cultures remained metabolically active, continuing to accumulate DNA, despite a cessation in the levels of RNA and cell protein accretion. In addition, the accumulation of glycogen and lipid contributed to the observed accretion of biomass in this phase. The depletion of nitrate also marked an increase in the production of a-ketoglutarate by the culture and a coincident decrease in medium pH. Latter stages of the secondary growth phase saw the development of spores within the culture, this in turn was associated with a decrease in cellular glycogen. This supported previous observations that glycogen degradation and spore maturation were intimately associated.

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