International Cooperation in Radiobiology through an Agency Sponsored by the United Nations

Eagle, Harry; Oyama, Vance I.; Levy, Mina; Freeman, Aaron E.

doi:10.1126/science.123.3202.845-a

Cited by 98 publications

(65 citation statements)

References 21 publications

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“…Lipoprotein lipase in newborn infants is usually estimated by determining postheparin plasma lipolytic activity (6,8). Postheparin plasma, however, contains hepatic lipase too (14,26).…”

Section: Discussionmentioning

confidence: 99%

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

1985

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ABSTRAm. The possibility that pulmonary surfactant, characterized by a phosphatidylglycerol deficiency, as in early fetal life, might have inferior surface properties was evaluated. We obtained this specific surfactant from adult rabbits by withholding glucose and giving them an excess of myoinositol by mouth and intravenously. Controls were given a similar quantity of glucose. The myoinositol resulted in a drastic reduction of surfactant phosphatidylglycerol, from 7.2 to 0.3% of phospholipids, and a corresponding increase in phosphatidylinositol from 4.8 to 11.3%. In addition, the myoinositol treatment increased the myoinosit01 that was disaturated from 18.5 to 27.3% ( p < 0.05).

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“…Lipoprotein lipase in newborn infants is usually estimated by determining postheparin plasma lipolytic activity (6,8). Postheparin plasma, however, contains hepatic lipase too (14,26).…”

Section: Discussionmentioning

confidence: 99%

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

1985

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“…Myoinositol, which is known to be a growth factor for some microorganisms and for a number of mammalian species, as well as for human cells in tissue culture (5). has been reported to be present at a higher concentration in fetal than in adult blood in a variety of mammalian species (2, 3).…”

Section: Speculationmentioning

confidence: 99%

Myoinositol in Human Neonates: Serum Concentrations and Renal Handling

et al. 1978

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3bound to the membrane (in that it resists washing out by the normal flow of secretions). A firmly bound effect was therefore sought by exposing normal red cells to saliva for 5 min, and then washing, before measuring ouabain-insensitive efflux. Table 2 shows that there was no difference between cells exposed to saliva and then washed, and cells not exposed to saliva. No firmly bound factor was demonstrated.The significance of humoral substances affecting membrane transport remains to be clarified. There does not appear to be any known hormone, for example, which could easily explain the above effect of saliva, whereas simple physical or physicochemical factors would seem to be excluded by the experimental controls. Modification of the cell membrane by uncharacterized circulating or secreted factors may have a bearing on transport processes in general, and possibly on the disturbance of CF in particular. However, the red cell model, as used in this study, has not confirmed that such a mechanism is important in CF. CONCLUSIONIn a preliminary study, the red blood cells of patients with cystic fibrosis were found to show decreased ouabain-insensitive Na+ efflux. The ouabain-sensitive Na+ efflux, total Na+ efflux, and Na+ influx were found to be normal. This information was used to guide the choice of transport parameters in the main study. The effect of cystic fibrosis saliva on normal red cells was not significantly different from that of control saliva, but both Pediat. Res. 12: 3-6 (1978) normal saliva and cystic fibrosis saliva appear to contain a substance which accelerates Na+ transport in the red cell.

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“…Eagle's tissue culture medium (Eagle, Oyama, Levy & Freeman, 1957) was chosen as a basis for compounding our medium. After various modifications of the original Eagle's medium a partially defined medium was achieved which enabled good multiplication of the saprophytic Mycoplasma laidlawii strains A and B.…”

Section: The Development Of the Partially Defined Mediummentioning

confidence: 99%

A Partially Defined Medium for the Growth of Mycoplasma

Razin

Knight

1960

Journal of General Microbiology

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SUMMARY: The saprophytic Mycoplasma laidlarevii strains A and B were grown in a partially defined medium composed of inorganic salts, Casamino acids, vitamins, nucleic acids, glucose and 10 yo (v/v) horse serum. The growth was less good than in the complex Edward medium; addition of known growth factors did not improve growth. Addition of yeast extract (0.05%, w/v) to the partially defined medium allowed growth equivalent to that in Edward medium. The serum could be replaced by 1 yo (w/v) crystallized bovine plasma albumin. The suboptimal growth obtained in the albumin-containing medium was not improved by adding cholesterol, Tween 80, lecithin and other growth factors known to be required by other Mycoplasma spp. Riboflavin and nicotinic acids were the only vitamins found to be essential for the growth of M. Zuidlawii strain A in the albumin medium. The Casamino acids could be replaced by a mixture of known amino acids. The pathogenic M. nu~coides var. Capri and M. bovigenitalium grew in the partially defined medium when the concentration of the serum was increased to 20 yo (v/v) and yeast extract added to a final concentration of 0.05 yo (w/v).

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International Cooperation in Radiobiology through an Agency Sponsored by the United Nations

Cited by 98 publications

References 21 publications

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

Myoinositol in Human Neonates: Serum Concentrations and Renal Handling

A Partially Defined Medium for the Growth of Mycoplasma

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