International Federation of Clinical Chemistry (IFCC): Scientific Division, Committee on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine (N‐phosphotransferase, EC 2.7.3.2). IFCC Recommendation

Hørder, Mogens; Elser, Robert C.; Gerhardt, W.; Mathieu, M.; Sampson, Eric J.

doi:10.1155/s1463924690000049

Cited by 58 publications

(45 citation statements)

References 21 publications

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“…AST, BUN, and CPK were measured according to the protocols of the International Federation of Clinical Chemistry, as described (43)(44)(45), by spectrophotometric analysis (modular DP; Roche-Hitachi, Echevarne Laboratories). Plasma HPX and haptoglobin were determined by ELISA (Life Diagnostics).…”

Section: Serum Biochemistrymentioning

confidence: 99%

A Central Role for Free Heme in the Pathogenesis of Severe Sepsis

et al. 2010

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Low-grade polymicrobial infection induced by cecal ligation and puncture is lethal in heme oxygenase-1-deficient mice (Hmox1(-/-)), but not in wild-type (Hmox1(+/+)) mice. Here we demonstrate that the protective effect of this heme-catabolizing enzyme relies on its ability to prevent tissue damage caused by the circulating free heme released from hemoglobin during infection. Heme administration after low-grade infection in mice promoted tissue damage and severe sepsis. Free heme contributed to the pathogenesis of severe sepsis irrespective of pathogen load, revealing that it compromised host tolerance to infection. Development of lethal forms of severe sepsis after high-grade infection was associated with reduced serum concentrations of the heme sequestering protein hemopexin (HPX), whereas HPX administration after high-grade infection prevented tissue damage and lethality. Finally, the lethal outcome of septic shock in patients was also associated with reduced HPX serum concentrations. We propose that targeting free heme by HPX might be used therapeutically to treat severe sepsis.

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Section: Serum Biochemistrymentioning

confidence: 99%

A Central Role for Free Heme in the Pathogenesis of Severe Sepsis

et al. 2010

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“…Creatine kinase was assayed according to the International Federation of Clinical Chemistry-approved method by CK-N-acetylcysteine kinetic measurement (37°C) with commercial reagents (Roche Diagnostics, Mannheim, Germany) on a Modular P Analyzer (Hitachi, Tokyo, Japan) [22]. The reference values in the normal population were 20 to 195 U/L for men and 20 to 170 U/L for women.…”

Section: Study Design and Patient Populationmentioning

confidence: 99%

Low serum creatine kinase activity is associated with worse outcome in critically ill patients

Moortel

Speeckaert

Fiers

et al. 2014

Journal of Critical Care

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“…The blood samples collected with heparinized bottles were centrifuged at 5000 rpm for 10 minutes to obtain clear plasma for the following investigations: Total, direct and indirect bilirubin were determined using Jandrassik and Grof technique (Koch and Doumas, 1982); alanine amino transferase (ALT) and aspatate amino transferase (AST) were measured by using enzymatic method (Horder and Sampson, 1991); alkaline phosphatase (ALP) was analyzed according to the method of Kind (1954); total protein (TP) concentration was determined by Biuret method (Doumas, 1975); albumin was determined based on its reaction with bromocresol green (Binding method) (Spencer and Price, 1971); urea was determined according to Urease-Berthelot method (Weatherburn, 1967) and plasma creatinine was estimated using Jaffe reaction (Perone et al, 1992).…”

Section: Biochemical Parametersmentioning

confidence: 99%

Acute and sub-chronic toxicity studies of the ethanol extract of the leaves of Sphenocentrum jollyanum (Menispermaceae)

Mbaka¹,

Adeyemi²,

Oremosu³

2010

ABJNA

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The acute toxicity study of the leaves of Sphenocentrum jollyanum (SJ) showed no toxicity when administered up to 11g/kg body weight orally while intra-peritoneal (IP) administration produced dose dependent mortality with median acute toxicity (LD 50 ) of 1445.4 mg/kg. In sub-chronic study, the extract administered for a period of 120 days showed no mortality or morbidity. There was significant weight gain in both treated and control groups while gross examination of internal organs revealed no detectable inflammation. The blood glucose and total cholesterol levels demonstrated dose dependent decrease while high density lipoprotein cholesterol (HDLcholesterol) increased with dose. In the liver function test, there were no significant (p>0.05) changes in alanine amino transferase (ALT) and aspertate amino transferase (AST) in the extract treated animals. The renal function profile, serum creatinine and urea levels were not significantly altered compared to the control. In haematological evaluation, significant increase (p<0.05) in red blood cells (RBC), packed cell volume (PCV) and haemoglobin (Hb) were observed in the treated animals compared to the control while mean corpuscular (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and white blood cells (WBC) showed no significant changes. The result therefore suggests that the leaves extract was potentially safe for consumption orally even at chronic administration.

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International Federation of Clinical Chemistry (IFCC): Scientific Division, Committee on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine (N‐phosphotransferase, EC 2.7.3.2). IFCC Recommendation

Abstract: Committee on Enzymes IFCC methods for the measurement of catalytic concentration enzymes Part 7. IFCC method for creatine kinase creatine N-phosphotransferase, EC 2.7.3.2). IFCC Recommendation

Cited by 58 publications

References 21 publications

A Central Role for Free Heme in the Pathogenesis of Severe Sepsis

A Central Role for Free Heme in the Pathogenesis of Severe Sepsis

Low serum creatine kinase activity is associated with worse outcome in critically ill patients

Acute and sub-chronic toxicity studies of the ethanol extract of the leaves of Sphenocentrum jollyanum (Menispermaceae)

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