International Symposium on Bioengineering

Purification of tubulin from neuroblastoma cells: Absence of covalently bound phosphate in tubulin from normal and morphologically differentiated cells

“…Second, the phosphate found associated with the tubulin may in fact be associated with a contaminant [16]. Third, there are reports of an in vitro phosphokinase activity which can utilize tubulin as an acceptor [6,17,18], and this activity may account for the phosphorylation found. Alternatively, loss of phosphate during purification cannot be ruled out.…”