Interphase fluorescence in situ hybridization in multiple myeloma and monoclonal gammopathy of undetermined significance without and with positive plasma cell identification: analysis of 192 cases from the Region of Southern Denmark

Christensen, Jacob Haaber; Abildgaard, Niels; Plesner, Torben; Nibe, Anne; Nielsen, Ole; Sørensen, Anne G.; Kerndrup, Gitte

doi:10.1016/j.cancergencyto.2006.11.015

Cited by 25 publications

(20 citation statements)

References 38 publications

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“…In our study, conventional cytogenetics detected chromosomal aberrations in 25% of our cohort of 68 MM patients, what is in line with results published by Christensen et al [8]. Analysis using interphase FISH combined with immunofluorescent labeling or immunomagnetic separation of plasma cells is known to have a higher sensitivity and finds chromosomal abnormalities in 86-98% of MM patients [9][10][11].…”

Section: Discussionsupporting

confidence: 92%

“…There is an evidence that del(17)(p13), which is found in approximately 10% of patients, is a marker of poor prognosis [8,20]. The incidence of del(17)(p13) in our cohort (9.8 %) was similar to that observed by others, that used 10% and 20% cut off, respectively.…”

Section: Discussionsupporting

confidence: 88%

“…The incidence of del(17)(p13) in our cohort (9.8 %) was similar to that observed by others, that used 10% and 20% cut off, respectively. [5,8]. According to recently published data, the presence of del (17)(p13) is associated with poor prognosis only if it is present in at least 60% of the malignant plasma cells [19].…”

Section: Discussionmentioning

confidence: 99%

“…Many prognostic factors for MM have been identified. The presence of numerical and/or structural chromosomal aberrations is one of the most important indicators for the prognostic assessments.Due to low proliferation activity of MM cells, conventional cytogenetics detects chromosomal changes in as few as 26-40% of cases [7,8]. In contrast, the technique of interphase fluorescent in situ hybridization (I-FISH) in combination with cytoplasm immunoglobulin staining or immunomagnetic separation of plasma cells enables detection of specific abnormalities in up to 86-98% of patients [9,10,11] …”

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confidence: 99%

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Negative prognostic significance of two or more cytogenetic abnormalities in multiple myeloma patients treated with autologous stem cell transplantation

Grešlíková

Zaoralová²,

Filková³

et al. 2010

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Multiple myeloma (MM) is the second most common haematological malignancy. It is characterized by malignant transformation and clonal proliferation of B-lymphocytes with the accumulation of malignant plasma cells in the bone marrow. The incidence of MM increases with age. The average age at presentation is 62 years in men and 61 years in women [1]. Patients below 40 years of age represent less than 3% of all cases. For many years, conventional chemotherapy with combination of melphalan and prednisone was the standard treatment, resulting in overall survival (OS) of approximately 3 years [2]. Currently, high-dose chemotherapy followed by autologous haematopoietic stem cell transplantation is recommended for patients below the age of 65 [3,4]. This treatment extends the OS to 4-5 years [5], with approximately 28% of patients surviving 5 or more years [6]. Despite this relative treatment success, MM remains an incurable disease. Many prognostic factors for MM have been identified. The presence of numerical and/or structural chromosomal aberrations is one of the most important indicators for the prognostic assessments.Due to low proliferation activity of MM cells, conventional cytogenetics detects chromosomal changes in as few as 26-40% of cases [7,8]. In contrast, the technique of interphase fluorescent in situ hybridization (I-FISH) in combination with cytoplasm immunoglobulin staining or immunomagnetic separation of plasma cells enables detection of specific abnormalities in up to 86-98% of patients [9,10,11] Received April 8, 2009Malignant plasma cells in multiple myeloma (MM) are frequently characterized by complex karyotypes and chromosome instability. These cytogenetic changes are considered important prognostic indicators in MM patients. We have studied samples from 68 patients with newly diagnosed MM who were treated with high-dose chemotherapy and autologous stem cell transplantation. G-banding revealed abnormal karyotypes in 14 of 55 patients (25%) who had informative conventional cytogenetics. The combination of cytoplasmic immunoglobulin light chain labeling and interphase fluorescent in situ hybridization (cIg-FISH) revealed the presence of genetic aberrations in 53 of 68 patients (78%). Chromosome 13 abnormalities were found in 33 patients (50%) and IgH rearrangements in 36 patients (56.25%). In IgH positive patients we performed subsequent examinations of IgH affecting translocations t(4;14) and t(11;14) and we found translocation t(11;14) in 8 patients (12.5%) and t(4;14) in 10 patients (15.5%). The occurrences of others chromosomal abnormalities with known prognostic impact in MM were as follows: del(17)(p13) was present in 5 patients (9.8%) and gain 1q21 in 14 patients (36%). Analysis of survival of patients with different cytogenetic abnormalities revealed shorter overall survival (OS) in patients with IgH rearrangements (p=0.020) and trend to shorter OS in patients with gain 1q21 (p=0.064), respectively. Remarkably, patients with two or more aberrations had significantly shorter overall surviva...

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Negative prognostic significance of two or more cytogenetic abnormalities in multiple myeloma patients treated with autologous stem cell transplantation

Grešlíková

Zaoralová²,

Filková³

et al. 2010

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“…N P 2 41,X,-X,+1,dic(1;2)(p13;p13),der(4)t(q11;p16),add(7)(p13),-11,-13,-14,-20,der(21;22)(q10;q10), N P P +mar [2] Table 3. Abnormal karyotypes and corresponding interphase FISH results of the patients with multiple myeloma 참고문헌…”

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confidence: 99%

Correlation of Chromosomal Aberrations with Prognostic Markers in Multiple Myeloma Patients-A Single Institution Study

Lee¹,

Lee²,

Hong³

et al. 2008

Ann Lab Med

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Background : Immunoglobulin heavy chain (IGH) gene rearrangement, 13q14 deletion and trisomy 1q are frequently observed in Korean patients with multiple myeloma. The purpose of our study was to analyze the statistical correlation between chromosomal aberrations and routine laboratory test results as prognostic markers and to evaluate the potential of chromosomal aberrations for the indirect assessment of prognosis in multiple myeloma patients.Methods : We investigated the prevalence of cytogenetic aberrations in 41 patients with newly diagnosed multiple myeloma. Cytogenetic analysis was conducted by conventional karyotyping and FISH for the presence of IGH/CCND1 translocation, 13q14 deletion, and trisomy 1q using bone marrow aspirates. The records of routine laboratory tests were reviewed and their correlation with cytogenetic abnormalities was investigated.Results : Sixteen (39.0%) of 41 patients had at least one cytogenetic abnormalities in conventional karyotyping or FISH. In FISH analysis of 37 patients, 8 (21.6%) showed positive result for IGH/CCND1 translocation, 8 (21.6%) for trisomy 1q, and 5 (13.5%) for 13q14 deletion. Cytogenetic abnormalities, especially trisomy 1q, were associated with significantly lower hemoglobin level and significant-

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The clinical and pathological features of plasma cell myeloma post solid organ transplantation

et al. 2020

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Plasma cell neoplasms (PCNs), comprising plasma cell myelomas (PCMs) and plasmacytomas, which occur after solid organ transplantation, represent rare subtypes of monomorphic post-transplant lymphoproliferative disorders (M-PTLDs). Data regarding the clinical and pathological features of post-transplant (PT)-PCMs are limited. To gain a better understanding of disease biology, we performed comprehensive immunophenotypic analysis, reviewed cytogenetic analysis results and evaluated clinical outcomes of PT-PCMs diagnosed and treated at our institution. Fifteen PT-PCM (M: F-4:1) and two PT-MGUS (two males) cases were identified. The median age of PT-PCM patients was 68 years (29-79 years) and PCMs presented at a median of 9.7 years (0.5-24.7 years) after transplantation. The PT-PCMs accounted for 11.6% of all M-PTLDs and the period prevalence was 9/3108 (0.29%), 3/1071 (0.28%), 2/1345 (0.15%) and 1/878 (0.11%) post kidney, heart, liver and lung transplantation. Lytic bone disease was observed in 1/11 (9%) patients. Marrow plasma cell infiltration ranged from 10%-70% (median 20%), with 10/15 (67%) and 5/15 (33%) cases manifesting immature and plasmablastic morphology. The immunophenotype of all cases and cytogenetic abnormalities, identified in 60% of cases, were similar to multiple myeloma (MM) of immunocompetent individuals. All PT-PCMs were EBER negative. Ten of 11 (91%) patients with active MM were treated, all with proteasome inhibitor-based therapy. Treatment response and 5-year overall survival (54.5%) was comparable to MM of immunocompetent individuals. However, the survival of patients with plasmablastic PCMs was inferior to those with immature PCMs. 0ur findings indicate PT-PCMs to be predominantly late onset PTLDs that have similar clinicopathologic characteristics as conventional MM.

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Interphase fluorescence in situ hybridization in multiple myeloma and monoclonal gammopathy of undetermined significance without and with positive plasma cell identification: analysis of 192 cases from the Region of Southern Denmark

Cited by 25 publications

References 38 publications

Negative prognostic significance of two or more cytogenetic abnormalities in multiple myeloma patients treated with autologous stem cell transplantation

Negative prognostic significance of two or more cytogenetic abnormalities in multiple myeloma patients treated with autologous stem cell transplantation

Correlation of Chromosomal Aberrations with Prognostic Markers in Multiple Myeloma Patients-A Single Institution Study

The clinical and pathological features of plasma cell myeloma post solid organ transplantation

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