Interplay among transacting factors around promoter in the initial phases of transcription

Kumar, Amarjeet; Chan, Justin; Taguchi, Masahiko; Kono, Hidetoshi

doi:10.1016/j.sbi.2021.04.008

Cited by 6 publications

(2 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experimental investigation of transcription initiation shows quite a resemblance to data access authorization. Biochemically, it involves cofactors whose binding is needed [3] and their interplay with the chromatin [4]. It is only recently that new techniques emerged to facilitate observation of DNA accessibility changes through chromatin compaction and phase separation [5].…”

Section: Information Processing Viewpointmentioning

confidence: 99%

An engineering perspective on transcription, translation and their regulation

Smieja

2024

Postepy Biochem

View full text Add to dashboard Cite

Information coded in DNA is replicated, modified and transmitted from the origins of protein-based life. Analogies of these processes to information processing, transmission and storage in computer systems is straightforward and can be utilized both in analysis of biological data and in development of biologically based technical systems. Transcription and translation processes are regulated by extremely complex regulatory networks, providing control of cell growth, cell cycle and cellular responses to stress. As such, they constitute engineering control systems exerting their actions at many levels of time scale and spatial organization. This work presents an engineering perspective on DNA-related information processing and biochemical process control in living cells, followed by a review of two-way crosstalk between engineering and biology.

show abstract

Section: Information Processing Viewpointmentioning

confidence: 99%

An engineering perspective on transcription, translation and their regulation

Smieja

2024

Postepy Biochem

View full text Add to dashboard Cite

show abstract

“…Gene expression is a highly complex process that underlies the fate and function of different cells and tissues. Regulation of this process consists of multiple horizontal molecular events [20][21][22]. The central event in regulating eukaryotic gene expression is the initiation of transcription by recruiting RNA polymerase II to the core promoter region [23].…”

Section: Rcfah12 Has Multiple Tsssmentioning

confidence: 99%

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Wang

et al. 2022

Separations

View full text Add to dashboard Cite

Castor (Ricinus communis L.) seed oil is the commercial source of ricinoleate, a valuable raw material used in many industries. Oleoyl-12-hydroxylase (RcFAH12) is a key enzyme in the biosynthesis of ricinoleate, accumulating nearly 90% of the triacylglycerol in castor seeds. Little is known about the transcriptional regulation of RcFAH12. We used rapid amplification of cDNA 5′ ends (5′RACE) to locate the transcription start site (TSS) of RcFAH12, and the sequence of a 2605 bp region, −2506~+99, surrounding the TSS was cloned. We then investigated these regions to promote β-glucuronidase (GUS) expression in transgenic Arabidopsis by the progressive 5′ and 3′ deletions strategies. The GUS staining showed that the GUS accumulation varied in tissues under the control of different deleted fragments of RcFAH12. In addition, the GUS expression driven by the RcFAH12 promoter markedly accumulated in transgenic seeds, which indicated that RcFAH12 might play an important role in the biosynthesis of ricinoleic acid. This study will lay a potential foundation for developing a tissue-specific promoter in oil-seed crops.

show abstract