2020
DOI: 10.1016/j.celrep.2020.107924
|View full text |Cite
|
Sign up to set email alerts
|

Interplay between Extracellular Matrix Stiffness and JAM-A Regulates Mechanical Load on ZO-1 and Tight Junction Assembly

Abstract: Summary Tight-junction-regulated actomyosin activity determines epithelial and endothelial tension on adherens junctions and drives morphogenetic processes; however, whether or not tight junctions themselves are under tensile stress is not clear. Here, we use a tension sensor based on ZO-1, a scaffolding protein that links the junctional membrane to the cytoskeleton, to determine if tight junctions carry a mechanical load. Our data indicate that ZO-1 is under mechanical tension and that forces actin… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

2
106
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
6
1
1

Relationship

1
7

Authors

Journals

citations
Cited by 69 publications
(108 citation statements)
references
References 50 publications
2
106
0
Order By: Relevance
“…Under actomyosin contraction, tension is created between the membrane-anchored tetraspanins bound to the NT portion and the actomyosin apparatus and actin-binding proteins (e.g., vinculin and α-catenin) bound to the CT portion. As a result, ZO1 can take on an open/closed conformation, regulating access to its central SH3-U5-GUK-U6 region to control protein binding (e.g., with occludin) and targeting signals (e.g., localization to the TJ complex) [ 76 ].…”
Section: Barrier-modulating Structuresmentioning
confidence: 99%
See 1 more Smart Citation
“…Under actomyosin contraction, tension is created between the membrane-anchored tetraspanins bound to the NT portion and the actomyosin apparatus and actin-binding proteins (e.g., vinculin and α-catenin) bound to the CT portion. As a result, ZO1 can take on an open/closed conformation, regulating access to its central SH3-U5-GUK-U6 region to control protein binding (e.g., with occludin) and targeting signals (e.g., localization to the TJ complex) [ 76 ].…”
Section: Barrier-modulating Structuresmentioning
confidence: 99%
“…Scaffolding proteins such as ZO1 and Vinculin have been shown to mediate the interaction between α-catenin and the actin cytoskeleton for the transmission of actomyosin push/pull forces at TJ-based barriers [ 76 , 103 , 104 , 105 ]. Actin contractile structures may contract radially or parallel to the plasma membrane, as cells tethered to neighboring cells by cell-cell junctions can dynamically form and break in a force-dependent manner [ 5 ].…”
Section: Barrier-modulating Structuresmentioning
confidence: 99%
“…Cytoskeletal remodeling and the thereby generated mechanical tension are thought to be key drivers of cell-cell fusion by promoting the close proximity of the neighboring plasma membranes required for fusion to occur (Cong et al, 2019;Kim et al, 2015). p114RhoGEF is required for tight junction assembly when monolayers are under high mechanical tension (Haas et al, 2020). As p114RhoGEF is required for PKA-stimulated remodeling of the actin cytoskeleton along cell-cell junctions prior to fusion, p114RhoGEF-deficient trophoblasts may not be able to generate and maintain the necessary close membrane-membrane contacts required for membrane fusion.…”
Section: P114rhogef Regulates Camp-induced Cell-cell Fusionmentioning
confidence: 99%
“…Given the role of p114RhoGEF in the regulation of dynamic cellular processes and the coordination of actomyosin activation in response to changes in cell adhesion in vitro (Acharya et al, 2018;Haas et al, 2020;Nakajima and Tanoue, 2011;Terry et al, 2012;Terry et al, 2011;Zihni et al, 2017), we asked whether such functions are important for organ morphogenesis. Our data show that p114RhoGEF is indeed essential for mouse development with embryos displaying a number of different phenotypes.…”
Section: Introductionmentioning
confidence: 99%
“…Tight junctions are important for maintaining the mechanical strength and permeability of the mucosal epithelium (Basler et al, 2016 ), which regulates the movement of ions and macromolecules between endothelial and epithelial cells (Lochhead et al, 2020 ). The tight junction protein ZO-1 is one important component that not only regulates cell material transport and maintains epithelial polarity, but also plays an important role in cell proliferation and differentiation and tumor cell invasion (Hsu et al, 2017 ; Haas et al, 2020 ).…”
Section: Introductionmentioning
confidence: 99%