Interplay between genetic regulation of phosphate homeostasis and bacterial virulence

Chekabab, Samuel M.; Harel, Josée; Dozois, Charles M.

doi:10.4161/viru.29307

Cited by 82 publications

(73 citation statements)

References 90 publications

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“…We chose phosphate starvation because it is a condition known to influence both the production of virulence factors and known Tat exoproteins1620. The extracellular 2D-gel profiles of WT and tat strains grown in rich medium did not show any visible differences and were not analyzed further.…”

Section: Resultsmentioning

confidence: 99%

Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

Ball¹,

Antelmann²,

Imbert³

et al. 2016

Sci Rep

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The opportunistic pathogen Pseudomonas aeruginosa uses secretion systems to deliver exoproteins into the environment. These exoproteins contribute to bacterial survival, adaptation, and virulence. The Twin arginine translocation (Tat) export system enables the export of folded proteins into the periplasm, some of which can then be further secreted outside the cell. However, the full range of proteins that are conveyed by Tat is unknown, despite the importance of Tat for the adaptability and full virulence of P. aeruginosa. In this work, we explored the P. aeruginosa Tat-dependent exoproteome under phosphate starvation by two-dimensional gel analysis. We identified the major secreted proteins and new Tat-dependent exoproteins. These exoproteins were further analyzed by a combination of in silico analysis, regulation studies, and protein localization. Altogether we reveal that the absence of the Tat system significantly affects the composition of the exoproteome by impairing protein export and affecting gene expression. Notably we discovered three new Tat exoproteins and one novel type II secretion substrate. Our data also allowed the identification of two new start codons highlighting the importance of protein annotation for subcellular predictions. The new exoproteins that we identify may play a significant role in P. aeruginosa pathogenesis, host interaction and niche adaptation.

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Section: Resultsmentioning

confidence: 99%

Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

Ball¹,

Antelmann²,

Imbert³

et al. 2016

Sci Rep

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“…The most common genes of the Pho regulon encode: extracellular enzymes capable of obtaining Pi from organic phosphates, Pi-specific transporters and enzymes involved in the storing and saving of nutrients (Rao & Torriani, 1990;Hsieh & Wanner, 2010;Santos-Beneit et al, 2008). The Pho regulon not only is a regulatory circuit of Pi homeostasis but also plays an important adaptive role in bacterial stress and virulence (Chekabab et al, 2014). Several new members of the Pho regulon have emerged in the last few years in several bacteria; however, there are still many unknown questions regarding the activation and function of the whole system (Santos-Beneit, 2015).…”

Section: Introductionmentioning

confidence: 99%

PhoB activation in non-limiting phosphate condition by the maintenance of high polyphosphate levels in the stationary phase inhibits biofilm formation in Escherichia coli

2016

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Polyphosphate (polyP) degradation in Escherichia coli stationary phase triggers biofilm formation via the LuxS quorum sensing system. In media containing excess of phosphate (Pi), high polyP levels are maintained in the stationary phase with the consequent inhibition of biofilm formation. The transcriptional-response regulator PhoB, which is activated under Pi limitation, is involved in the inhibition of biofilm formation in several bacterial species. In the current study, we report, for the first time, we believe that E. coli PhoB can be activated in non-limiting Pi conditions, leading to inhibition of biofilm formation. In fact, PhoB was activated when high polyP levels were maintained in the stationary phase, whereas it remained inactive when the polymer was degraded or absent. PhoB activation was mediated by acetyl phosphate with the consequent repression of biofilm formation owing to the downregulation of c-di-GMP synthesis and the inhibition of autoinducer-2 production. These results allowed us to propose a model showing that PhoB is a component in the signal cascade regulating biofilm formation triggered by fluctuations of polyP levels in E. coli cells during stationary phase.

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“…Many possible conditions, such as nutrient shortage or stress, may be involved (Martin‐Verstraete et al ., ) and among these, we favor a role of inorganic phosphate (P i ). Commonly, changes in environmental P i ‐concentration activate the Pho‐regulon which is not only involved in intracellular phosphate homeostasis, but also modulates virulence (Chekabab et al ., ). In general, the Pst system, which is involved in the transport of P i , is necessary for P i signal transduction and forms a link with the PhoR/PhoB two component‐system (Hsieh and Wanner, ).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Covalent attachment and Pro‐Pro endopeptidase (PPEP‐1)‐mediated release of Clostridium difficile cell surface proteins involved in adhesion

Corver

Cordó

Leeuwen

et al. 2017

Molecular Microbiology

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In the past decade, Clostridium difficile has emerged as an important gut pathogen. This anaerobic, Gram-positive bacterium is the main cause of infectious nosocomial diarrhea. Whereas much is known about the mechanism through which the C. difficile toxins cause diarrhea, relatively little is known about the dynamics of adhesion and motility, which is mediated by cell surface proteins. This review will discuss the recent advances in our understanding of the sortase-mediated covalent attachment of cell surface (adhesion) proteins to the peptidoglycan layer of C. difficile and their release through the action of a highly specific secreted metalloprotease (Pro-Pro endopeptidase 1, PPEP-1). Specific emphasis will be on a model in which PPEP-1 and its substrates control the switch from a sessile to motile phenotype in C. difficile, and how this is regulated by the cyclic dinucleotide c-di-GMP (3'-5' cyclic dimeric guanosine monophosphate).

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Interplay between genetic regulation of phosphate homeostasis and bacterial virulence

Cited by 82 publications

References 90 publications

Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

PhoB activation in non-limiting phosphate condition by the maintenance of high polyphosphate levels in the stationary phase inhibits biofilm formation in Escherichia coli

Covalent attachment and Pro‐Pro endopeptidase (PPEP‐1)‐mediated release of Clostridium difficile cell surface proteins involved in adhesion

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