Interplay between interferon-stimulated gene 15/ISGylation and interferon gamma signaling in breast cancer cells

Tecalco-Cruz, Ángeles C.; Cortés-González, Carlo César; Cruz-Ramos, Eduardo; Jarquín, Josué O. Ramírez; Romero-Mandujano, Aline Kay; Sosa-Garrocho, Marcela

doi:10.1016/j.cellsig.2018.11.021

Cited by 19 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we cannot exclude that the in vitro culture conditions used in the present study enhance LRRK2 expression and cytokine production. In line with its role in cytoskeletal remodeling 63 , IFN-γ stimulation reduced the motility of microglia. However, such an effect was significant only in LRRK2 G2019S microglia, suggesting that PD LRRK2 mutations enhance immune responses to IFN-γ.…”

Section: Discussionmentioning

confidence: 67%

“…contributes to numerous brain functions including neurogenesis, neurite outgrowth, and maintenance of neurons 40 . Interestingly, IFN-γ leads to cytoskeleton reorganization that may be partially mediated by IQGAP1 63 . As hypothesized, our knockdown experiments show that IQGAP1 is involved in LRRK2 and IFN-γmediated negative regulation of NFAT shuttling.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Interferon-γ signaling synergizes with LRRK2 in human neurons and microglia

Cicco

Ivanyuk

Haq

et al. 2020

Preprint

View full text Add to dashboard Cite

Increasing evidence suggests a role for interferons (IFNs) in neurodegeneration.Parkinson's disease (PD) associated kinase LRRK2 has been implicated in IFN type II (IFN) response in infections and nigral neuronal loss. However, whether and how LRRK2 synergizes with IFN-γ still remains unclear. Here, we employed dopaminergic (DA) neurons and microglia differentiated from patient induced pluripotent stem cells to unravel the role of IFN-γ in LRRK2-PD. We show that IFN-γ induces LRRK2 expression in both DA neurons and microglial cells. LRRK2-G2019S, the most common PD-associated mutation, sensitizes DA neurons to IFN-γ by decreasing AKT phosphorylation. IFN-γ suppresses NFAT activity in both neurons and microglia and synergistically enhances LRRK2-induced defects of NFAT activation.Furthermore, LRRK2-G2019S negatively regulates NFAT via calcium and microtubule dynamics. Importantly, we uncover functional consequences of the reduction of NFAT activity in both cell types, namely defects of neurite elongation and alteration of microglial activation profile and motility. We propose that synergistic IFN-γ/LRRK2 activation serves as a direct link between inflammation and neurodegeneration in PD.

show abstract

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Interferon-γ signaling synergizes with LRRK2 in human neurons and microglia

Cicco

Ivanyuk

Haq

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…ISG15 can disrupt cytoskeletal architecture and promote motility in human breast cancer cells [56,57]. Cytoskeleton remodelling and associated signalling was found to be regulated by the isgylation of IQGAP1 [58,59], non-muscle myosin IIA [60] and filamin B [61]. The activation of Stat1 by focal adhesion kinase FAK is involved in integrin-mediated cell migration and adhesion [62,63].…”

Section: Discussionmentioning

confidence: 99%

Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells

Gandalovičová

Šůchová

Čermák

et al. 2020

Cancers

View full text Add to dashboard Cite

The invasive behaviour of cancer cells underlies metastatic dissemination; however, due to the large plasticity of invasion modes, it is challenging to target. It is now widely accepted that various secreted cytokines modulate the tumour microenvironment and pro-inflammatory signalling can promote tumour progression. Here, we report that cells after mesenchymal–amoeboid transition show the increased expression of genes associated with the type I interferon response. Moreover, the sustained activation of type I interferon signalling in response to IFNβ mediated by the Stat1/Stat2/IRF9 complex enhances the round amoeboid phenotype in melanoma cells, whereas its downregulation by various approaches promotes the mesenchymal invasive phenotype. Overall, we demonstrate that interferon signalling is associated with the amoeboid phenotype of cancer cells and suggest a novel role of IFNβ in promoting cancer invasion plasticity, aside from its known role as a tumour suppressor.

show abstract

“…In patients with breast cancer, ISG15 is overexpressed at both the mRNA and protein levels in mammary tumor tissue compared to that in normal mammary tissue. 5,6 Increased ISG15 protein expression has also been detected in human melanoma cell lines. 7 These findings indicate the potential of ISG15 as a tumor biomarker.…”

Section: Illustrative Casementioning

confidence: 99%

Organizing gene literature retrieval, profiling, and visualization training workshops for early career researchers

et al. 2021

View full text Add to dashboard Cite

Developing the skills needed to effectively search and extract information from biomedical literature is essential for early-career researchers. It is, for instance, on this basis that the novelty of experimental results, and therefore publishing opportunities, can be evaluated. Given the unprecedented volume of publications in the field of biomedical research, new systematic approaches need to be devised and adopted for the retrieval and curation of literature relevant to a specific theme. Here we describe a hands-on training curriculum aimed at retrieval, profiling, and visualization of literature associated with a given topic. This curriculum was implemented in a workshop in January 2021. We provide supporting material and step-by-step implementation guidelines with the ISG15 gene literature serving as an illustrative use case. Through participation in such a workshop, trainees can learn: 1) to build and troubleshoot PubMed queries in order to retrieve the literature associated with a gene of interest; 2) to identify key concepts relevant to given themes (such as cell types, diseases, and biological processes); 3) to measure the prevalence of these concepts in the gene literature; 4) to extract key information from relevant articles, and 5) to develop a background section or summary on the basis of this information. Finally, trainees can learn to consolidate the structured information captured through this process for presentation via an interactive web application.

show abstract

Interplay between interferon-stimulated gene 15/ISGylation and interferon gamma signaling in breast cancer cells

Cited by 19 publications

References 54 publications

Interferon-γ signaling synergizes with LRRK2 in human neurons and microglia

Interferon-γ signaling synergizes with LRRK2 in human neurons and microglia

Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells

Organizing gene literature retrieval, profiling, and visualization training workshops for early career researchers

Contact Info

Product

Resources

About