Interplay between surface properties of standard, vitamin E blended and oxidised ultra high molecular weight polyethylene used in total joint replacement and adhesion ofStaphylococcus aureusandEscherichia coli

Abstract

AMInterplay between surface properties of standard, vitamin E blended and oxidised ultra high molecular weight polyethylene used in total joint replacement and adhesion of Staphylococcus aureus and Escherichia coli. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME (2014) 96-B (4) DOI: 10.1302/0301-620X.96B4/32895The definitive version is available at:http://www.bjj.boneandjoint.org.uk/cgi/doi/10.1302/0301-620X.96B4/32895 AbstractThis study was aimed at assessing the different adhesive strength of some of the most common bacteria associated with periprosthetic infection on various types of Ultra High Molecular Weight Polyethylene (UHMWPE) components. Quantitative in vitro analysis of the adhesion of biofilm producing strains of Staphylococcus aureus and Escherichia coli to physico-chemically characterized standard UHMWPE (PE), Vitamin E blended UHMWPE (VE-PE) and oxidized UHMWPE (OX-PE) was performed by using a sonication protocol. A significant decreased bacterial adhesion was registered for both strains on VE-PE, in comparison with that observed on PE, within 48h of observation (S. aureus p=0.0243 and E. coli p=0.0081). Biomaterial associate infections are still an issue in total joint replacement where often result in a series of implant-related sequelae that can lead to implant removal with clinical and economic consequences of significant importance. Since Vitamin E reduces bacterial adhesive ability, VE-stabilized UHMWPE could be a joint arthroplasty valid technology by presenting excellent quality on mechanical, wear, oxidation and adhesion properties.

Prevention of bacterial adhesion and biofilm formation on a vitamin E-blended, cross-linked polyethylene surface with a poly(2-methacryloyloxyethyl phosphorylcholine) layer

“…For example, Banche et al and Gómez-Barrena et al reported that vitamin E-doped or -blended PE had the potential to reduce bacteria adhesion [12][13][14]. However, vitamin E blending has also been shown to reduce bacterial adhesion by only about one-tenth or less [12][13][14][15][16]. Moreover, Williams et al reported that vitamin E-blended PE did not have the ability to reduce biofilm formation [16], which was consistent with the results of this study.…”

“…Previous studies evaluated bacteria adherence to vitamin E-blended PE [12][13][14][15][16]. For example, Banche et al and Gómez-Barrena et al reported that vitamin E-doped or -blended PE had the potential to reduce bacteria adhesion [12][13][14].…”

“…Previous studies evaluated bacteria adherence to vitamin E-blended PE [12][13][14][15][16]. For example, Banche et al and Gómez-Barrena et al reported that vitamin E-doped or -blended PE had the potential to reduce bacteria adhesion [12][13][14]. However, vitamin E blending has also been shown to reduce bacterial adhesion by only about one-tenth or less [12][13][14][15][16].…”

“…Recently, several studies have reported that the antioxidant vitamin E (a-tocopherol) blended with polyethylene (PE) may have the potential to reduce bacterial adhesion [12][13][14][15]. In particular, vitamin E and vitamin E-blended PE have been shown to disrupt chromic infection through induction of specific cellular immune responses.…”

“…However, a separate study showed that vitamin E-blended PE does not reduce biofilm formation [16]. From these studies, the mechanisms through which vitamin E may affect bacterial adhesion and the clinical efficacy of vitamin E in bacterial adhesion and biofilm formation are not clear because vitamin E blended PE only reduces bacterial adhesion by 10% or less [12][13][14][15][16].…”