Interplay of transcription factors and microRNAs during embryonic hematopoiesis

Gong, Xueping; Chao, Robert; Wang, Pengxiang; Huang, Xiaoli; Zhang, Jingjing; Zhu, Xiaozhou; Zhang, Yanyang; Yang, Xue; Hou, Can; Ji, Xiangjun; Shi, Tieliu; Wang, Yuan

doi:10.1007/s11427-016-0168-0

Cited by 10 publications

(11 citation statements)

References 47 publications

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“…Also, in order to verify the validity of RNA sequencing results, reproducibility was representatively ascertained by real‐time PCR (see supplemental methods and Figure S8). In fact, the majority of miRNAs that were defined in the present study as most valuable in reliable ABT detection, was already brought up in the context of hematopoiesis 22,84 . Additionally, miR‐144‐3p was not only associated with erythroid differentiation, but was also described as useful long‐term marker in EPO abuse 27,74 .…”

Section: Discussionmentioning

confidence: 63%

“…In fact, the majority of miRNAs that were defined in the present study as most valuable in reliable ABT detection, was already brought up in the context of hematopoiesis. 22,84 Additionally, miR-144-3p was not only associated with erythroid differentiation, but was also described as useful long-term marker in EPO abuse. 27,74 Further, in silico analysis using TargetScan (v7.2; www.targetscan.org) 85 established the EPO receptor as target of the miR-320 family, miR-4646-3p, and miR-223-5p, while miR-146b-3p seems to target EPO directly.…”

Section: The Potential Of Whole Blood Mirna Profiles In Abt Detectionmentioning

confidence: 99%

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On the trail of blood doping—microRNA fingerprints to monitor autologous blood transfusions in vivo

Mussack

Wittmann²,

Pfaffl

2021

American J Hematol

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Autologous blood doping refers to the illegal re‐transfusion of any quantities of blood or blood components with blood donor and recipient being the same person. The re‐transfusion of stored erythrocyte concentrates is particularly attractive to high‐performance athletes as this practice improves their oxygen capacity excessively. However, there is still no reliable detection method available. Analyzing circulating microRNA profiles of human subjects that underwent monitored autologous blood transfusions seems to be a highly promising approach to develop novel biomarkers for autologous blood doping. In this exploratory study, we randomly divided 30 healthy males into two different treatment groups and one control group and sampled whole blood at several time points at baseline, after whole blood donation and after transfusion of erythrocyte concentrates. Hematological variables were recorded and analyzed following the adaptive model of the Athlete Biological Passport. microRNA profiles were examined by small RNA sequencing and comprehensive multivariate data analyses, revealing microRNA fingerprints that reflect the sampling time point and transfusion volume. Neither individual microRNAs nor a signature of transfusion‐dependent microRNAs reached superior sensitivity at 100% specificity compared to the Athlete Biological Passport (≤11% 6 h after transfusion versus ≤44% 2 days after transfusion). However, the window of autologous blood doping detection was different. Due to the heterogenous nature of doping, with athletes frequently combining multiple medications in order to both gain a competitive advantage and interfere with known testing methods, the true applicability of the molecular signature remains to be validated in real anti‐doping testings.

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Section: Discussionmentioning

confidence: 63%

Section: The Potential Of Whole Blood Mirna Profiles In Abt Detectionmentioning

confidence: 99%

On the trail of blood doping—microRNA fingerprints to monitor autologous blood transfusions in vivo

Mussack

Wittmann²,

Pfaffl

2021

American J Hematol

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“…Although miRNAs play an important role in the regulation and maintenance of normal physiological conditions, in pathological situations, their levels may change due to dysfunction [14, 32–34]. Consequently, findings regarding the function of miRNAs in OA have varied depending on their expression and the genes targeted [17, 35].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

Duan

Huang

et al. 2019

BioMed Research International

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Background and Aims. A growing body of research has demonstrated that the degeneration of chondrocytes is the primary cause of osteoarthritis (OA). Parathyroid hormone-related protein (PTHrP) can alleviate the degeneration of chondrocytes via promotion of chondrocyte proliferation and inhibition of terminal differentiation, but the underlying mechanism remains unknown. This study aimed to identify the microRNAs (miRNAs) that may target PTHrP and regulate the proliferation and terminal differentiation of chondrocytes. Methods. Bioinformatic analysis was used to predict which miRNAs target PTHrP. We collected human knee cartilage specimens to acquire the primary chondrocytes, which we then used to test the expression and function of the targeted miRNAs. To explore the effects of miR-15a-5p on the putative binding sites, specific mimics or inhibitors were transfected into the chondrocytes. Furthermore, a dual-luciferase reporter gene assay and chondrocyte degeneration-related factors were used to verify the possible mechanism. Results. The expression of PTHrP was upregulated in the OA chondrocytes, whilst miR-15a-5p was downregulated in the OA chondrocytes. A negative correlation was observed between PTHrP and miR-15a-5p. The knockdown of miR-15a-5p promoted the growth of chondrocytes and inhibited calcium deposition, whilst overexpression of miR-15a-5p reversed this trend. The effect of miR-15a-5p overexpression was neutralised by PTHrP. Dual-luciferase reporter assays revealed that PTHrP can be used as a novel targeting molecule for miR-15a-5p. Conclusions. miR-15a-5p promotes the degeneration of chondrocytes by targeting PTHrP and, in addition to helping us understand the development of OA, may be a potential biomarker of OA.

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“…Total RNAs were extracted with Trizol (Thermo Fisher Scientific), and cDNAs were synthesized using a PrimeScript ® RT reagent Kit (TaKaRa) following manufacturer's protocols, as previously described. 43,44 The primers used in this assay are provided in the Table S1.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

CAP1, a target of miR‐144/451, negatively regulates erythroid differentiation and enucleation

Huang

Chao

Zhang

et al. 2021

J Cellular Molecular Medi

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The exact molecular mechanism underlying erythroblast enucleation has been a fundamental biological question for decades. In this study, we found that miR‐144/451 critically regulated erythroid differentiation and enucleation. We further identified CAP1, a G‐actin‐binding protein, as a direct target of miR‐144/451 in these processes. During terminal erythropoiesis, CAP1 expression declines along with gradually increased miR‐144/451 levels. Enforced CAP1 up‐regulation inhibits the formation of contractile actin rings in erythroblasts and prevents their terminal differentiation and enucleation. Our findings reveal a negative regulatory role of CAP1 in miR‐144/451‐mediated erythropoiesis and thus shed light on how microRNAs fine‐tune terminal erythroid development through regulating actin dynamics.

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Interplay of transcription factors and microRNAs during embryonic hematopoiesis

Cited by 10 publications

References 47 publications

On the trail of blood doping—microRNA fingerprints to monitor autologous blood transfusions in vivo

On the trail of blood doping—microRNA fingerprints to monitor autologous blood transfusions in vivo

MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

CAP1, a target of miR‐144/451, negatively regulates erythroid differentiation and enucleation

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