Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

Abstract

Summary. Four European centres provided height and weight data on 202 males and 204 females undergoing red cell mass (RCM) and plasma volume (PV) measurements. For these populations, the RCM and PV predictions by the various published methods were compared. It was shown clearly that predictions based solely on body weight were inappropriate, particularly because approximately half of the male and female populations could be regarded as overweight or obese. Although there was reasonable agreement in the prediction values given by the formulae based on both height and weight, it was not possible to establish which formulae could be recommended. For that reason. the published literature containing normal RCM and PV measurements were re-examined. RCM data for 283 males and 171 females and PV data for 100 males and 67 females were included. Measurements were standardized for variables such as trapped plasma in the PCV, exclusion of buffy coat in the PCV and calculation of PV at zero time. As a result of this analysis, prediction formulae based on surface area for RCM and PV with 98/99% reference ranges have been established. Keywords: red cell mass, plasma volume, normal values, interpretation.Recommended methods for the measurement of red cell mass (RCM) and plasma volume (PV) have been drawn up by the Radionuclide Panel of the International Committee for Standardization in Haematology (1 980b). These methods are now widely followed and provide within-method accuracy in the order of 2-3%.The remaining problem with RCM and PV measurements is the interpretation of the results. Traditionally, results and normal values have been expressed in terms of ml per kg total body weight. Indeed, the widely quoted Polycythaemia

Lower vascular tone and larger plasma volume in Parkinson's disease with orthostatic hypotension

“…Measured plasma volume was expressed according to the calculated lean body mass [men: 1.10·weight Ϫ 128·(weight 2 /100·height 2 ), women: 1.07·weight Ϫ 148·(weight 2 /100·height 2 ) (24)]. A predicted plasma volume was calculated based on sex, body weight, and height (36).…”

Increased Erythrocyte Count on Top of Bone Marrow Histology but not Serum EPO Level or JAK2 Mutation Load Discriminates between JAK2V617F Mutated Essential Thrombocythemia and Polycythemia Vera

“…These results indicate that bone marrow histopathology on its own is not reliable to differentiate between ET and PV but appeared to be a powerful tool to differentiate ET and PV from all variants of primary or secondary erythrocytosis and reactive thrombocytosis with a sensitivity and specificity near to 100% [5,6] [17]. Hb levels are normal in PT and elevated in PV [16]. Leukocytes were normal or elevated in PT and PV.…”

“…The clinical features of erythromelalgia and transient neurologic and ocular ischemic manifestations caused by platelet-mediated arteriolar inflammation and thrombosis in thrombocythemia in 26 MPN (ET and PV cases A in Table 4) have been reported in great detail [1][2][3]. Eleven of 14 ET patients had platelet counts between 400 and 1000 × 10 thrombocythemia, (30 ET and 20 PV) are described in great detail [16]. The peripheral blood findings in 30 ET (primary thrombocythemia; PT) and 20 PV seen between 1975 and 1985 are shown in Figure 1 [17].…”

“…All bone marrow biopsies were evaluated by expert hematopathologists for morphology, grading of cellularity and scoring of silver stained reticulin fibers according to PVSG (Ellis et al [13], Table 1C) and WHO recommendations [14]. On top of JAK2 V617F mutation screening using the PCR test according to Baxter et al [15], and quantitative JAK2 mutation allele burden, the diagnostic work-up of ET and PV patients included serum erythropoietin (EPO) levels, bone marrow aspirate for morphology and endogenous erythroid colony formation (EEC), red cell mass (RCM) measurement according to Pearson et al [16] and spleen size on echogram. The minimum criterion for the diagnosis of ET or thrombocythemia associated with PV is 400 × 10 9 / L [1][2][3][4][5][6][7][8][9][10] .…”

Diagnostic Performance and Prognostic Value of Extravascular Retention of 123I-Labeled Serum Amyloid P Component in Systemic Amyloidosis

“…Trichloroacetic acid-precipitable (proteinbound) radioactivity in plasma was used to measure the percentage of the injected dose still present in the vascular compartment, by using a calculated estimate of the plasma volume (16). The blood volume (in mL) was calculated by applying an algorithm using sex, body weight (in kg), and height (in m) (17): male blood volume 5 (366.9 · height 3 ) 1 (32.19 · weight) 1 604, and female blood volume 5 (356.1 · height 3 ) 1 (33.08 · weight) 1 183.3.…”