Interralations between nucleic acid and protein biosynthesis I. Synthesis and fate of bacterial nucleic acids during exposure to, and recovery from the action of chloramphenicol

Hahn, Fred E.; Schaechter, Moselio; Ceglowski, Walter S.; Hopps, Hope E.; Ciak, Jennie

doi:10.1016/0006-3002(57)90092-6

Cited by 54 publications

(15 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The synthesis of DNA, however, continues (13,43). It has already been shown that the nucleoids of treated cells undergo a transformation leading to specific vesicular structure (19,23,24).…”

Section: (B) ]Vudeoids Of Starved Bacteria--growingmentioning

confidence: 99%

Electron Microscope Study of DNA-Containing Plasms

Kellenberger

Ryter

Séchaud

1958

The Journal of Cell Biology

976

136

View full text Add to dashboard Cite

The nucleoids of Eseherichia coil, independently of the physiological state of the bacteria, are shown to be preserved as a fine-stranded fibrillar nucleoplasm by an OsO4 fixation under defined conditions: acetate-veronal buffer pH 6, presence of Ca ++ and amino acids, stabilization with uranyl-acetate before dehydration. The same fixation procedure applied to the DNA of vegetative phage reveals a pool of homogeneous fibrillar structure very similar to the nucleoplasm. The "versene test," which produces a coarse coagulation of these plasms, emphasizes the similar behaviour of the pool and the nucleoids.The heads of mature phage are preserved in their true polyhedral shape by the standard fixation procedure, although they may be badly distorted when fixed under different conditions. Lanthanum nitrate and uranyl-acetate are shown to increase markedly the contrast of both phage and cytoplasm.The consequences of the fibrillar structure of the genetic material are discussed in relation to the probable division process.

show abstract

Section: (B) ]Vudeoids Of Starved Bacteria--growingmentioning

confidence: 99%

Electron Microscope Study of DNA-Containing Plasms

Kellenberger

Ryter

Séchaud

1958

The Journal of Cell Biology

976

136

View full text Add to dashboard Cite

show abstract

“…In earlier work on antibiotic-induced lag it was reported (Hahn et al 1957; Neidhart & Gros, 1957; Horowitz et al 1958) that the RNA which accumulated was degraded and excreted before growth resumed. It was suggested by these workers that this excretion was essential to the recovery process.…”

Section: Loss Of Antibiotic-accumulated Rna May Occur Under Some Condmentioning

confidence: 99%

“…Several years ago there were independent reports from several laboratories of accumulation of RNA and its subsequent degradation in chloramphenicol-inhibited cells (Hahn et al 1957; Neidhardt & Gros, 1957; Horowitz, Lombard & Chargaff, 1958). The first two groups of workers showed that cells which had been treated with antibiotic, and had accumulated RNA, exhibited a lag before growth resumed when they were suspended in antibiotic-free medium.…”

Section: Introductionmentioning

confidence: 99%

RNA Synthesis and Degradation during Antibiotic Treatment and its Relation to Antibiotic-induced Lag

1961

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARYWhen growing cells of Escherichia coli were treated with chloramphenicol or erythromycin for 1 hr. and then suspended in antibiotic-free medium, there was a 45 min. lag before growth resumed. By eliminating growth factors or other essential nutrients during antibiotic treatment, it was possible to show that the lag occurred only when ribonucleic acid (RNA) synthesis could take place and did not require the synthesis of deoxyribonucleic acid (DNA). This antibiotic-induced RNA was apparently abnormal and was degraded when the antibiotic was removed. This degradation is a hydrolytic process and does not require the presence of a complete growth medium. During the recovery from the antibiotic-induced lag, DNA and protein synthesis did not occur, but RNA synthesis occurred, even though this new RNA synthesis was not required for the lag to be overcome. When antibiotictreated cells were suspended in phosphate buffer, a decrease in optical density of the suspension occurred which resembled a lytic process, but lysis apparently did not occur. Although these results clarify considerably earlier observations on antibioticinduced lag, they leave unsolved the question of why the antibiotic-induced RNA is abnormal, and how it brings about the lag.

show abstract

“…Guinea pig serum was used in their study and even though the characteristic "rabbit ear" effect was not noticed, protoplasts were apparently formed. The "rabbit ear" effect referred to cell aberrations obtained by spheroplast induction with penicillin, as published by HAHN and CIAK (1957) and LEDERBERG (1957). MuscHEL, et al (1959) postulated a two stage process " .... in which the antibody-complement system paves the way for lysozyme action that results in protoplasts".…”

mentioning

confidence: 99%

Antibiotics

1967

View full text Add to dashboard Cite

AII rights, especially that of tnmslation into foreign languages, reserved. It is also forbidden to reproduce this book, either whole DedicationFor most areas of scientific pursuit, there is usually that rare investigator who has the imagination to conceive ideas, who has faith in his visions, and who has the ability to critically test his concepts in the laboratory. Almost invariably, this scientist also inspires younger men to enthusiastically enter into his research program. To him should go the accolades and the recognitions of the esteem in which he is held. As only a small part of this esteem, we wish to dedicate these books to Professor SELMAN A. W AKSMAN in appreciation of his leadership and contributions in all facets of antibiotic research. PrefaceThe idea for publishing these books on the mechanism of action and on the biosynthesis of antibiotics was born of frustration in our attempts to keep abreast of the literature. Gone were the years when we were able to keep a bibliography on antibiotics and feel confident that we could find everything that was being published on this subject. These fields of investigation were moving forward so rapidly and were encompassing so wide a range of specialized areas in microbiology and chemistry that it was almost impossible to keep abreast of developments. In our naivete and enthusiasm, however, we were unaware that we were toying with an idea that might enmesh us, that we were creating an entity with a life of its own, that we were letting loose a Golom who instead of being our servant would be our master.That we set up ideals for these books is obvious; they would be current guides to developments and information in the areas of mechanism of action and biosynthesis of antibiotics. For almost every subject, we wished to enlist the aid of an investigator who himself had played a part in determining the nature of the phenomena that were being discussed. One concept for the books was that they include only antibiotics for which a definitive, well-documented mechanism of action or biosynthetic pathway was known. Yet, such an approach would not entirely serve the purpose we projected, for it would not encompass all of the information available in these fields of antibiotic investigations and blind searches for the original literature would still have to be made. We therefore chose to include any and all antibiotics about which some pertinent information had been published. It was obvious even at the start that such a compilation, integration, and analysis of information could never be complete unless scientific investigations ceased at the moment the last manuscript was submitted-an end that was neither desirable nor possible. An addendum was therefore included at the end of the volume and left open for the addition of new information until the last pages of the regular articles had been printed.The original concept has also been further expanded as the authors of several papers found it advisable to elaborate on their subject. Some of the articles included discussions...

show abstract

Interralations between nucleic acid and protein biosynthesis I. Synthesis and fate of bacterial nucleic acids during exposure to, and recovery from the action of chloramphenicol

Cited by 54 publications

References 15 publications

Electron Microscope Study of DNA-Containing Plasms

Electron Microscope Study of DNA-Containing Plasms

RNA Synthesis and Degradation during Antibiotic Treatment and its Relation to Antibiotic-induced Lag

Antibiotics

Contact Info

Product

Resources

About