Interrelated Effects of Zinc Deficiency and the Microbiome on Group B Streptococcal Vaginal Colonization

Burcham, Lindsey R.; Burcham, Zachary M.; Akbari, Madeline S.; Metcalf, Jessica L.; Doran, Kelly S.

doi:10.1128/msphere.00264-22

Cited by 9 publications

(7 citation statements)

References 42 publications

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“…Outcomes among the Chow-fed mice also diverged from rLFD and rHFD female mice upon GBS colonization. While all three cohorts developed high-density GBS colonization–which tended to overtake all non-GBS taxa, consistent with results from others (87, 88)—the Chow-fed mice cleared GBS vaginal colonization at higher rates than either of the refined diet groups. Given that the major distinguishing feature of the Chow and rLFD diets is the soluble fiber content, we hypothesize that fiber intake influences vaginal microbiota composition, favoring persistence of taxa that potentiate clearance of GBS.…”

Section: Discussionsupporting

confidence: 89%

“…In contrast to humans, individual variation in mouse vaginal microbiota is determined by host genetics, strain, and vendor (82,83). Staphylococcus, Enterococcus, and Rodentibacter species (formerly Pasteurella pneumotropica) typically dominate in the C57Bl/6 mouse vagina (83)(84)(85)(86), while CD-1 mice have vaginal microbiota dominated by Enterobacteriaceae and Proteus species (87). Additionally, the current study used mice from Taconic and these C57Bl/6 mice did not exhibit presence of vaginal Rodentibacter, compared to our previous work using C57Bl/6 mice from Jackson Laboratories where Rodentibacter was a dominant taxa (84,86), pointing to important vendor differences in shaping rodent vaginal microbiota composition Recent efforts have established a mouse CST (mCST) system analogous to the human classification, with five different mCST.…”

Section: Discussionmentioning

confidence: 99%

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Diet influences community dynamics following vaginal group B Streptococcus colonization

Megli,

DePuyt,

Goff

et al. 2023

Preprint

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The vaginal microbiota plays a pivotal role in reproductive, sexual, and perinatal health and disease. Unlike the well-established connections between diet, metabolism, and the intestinal microbiota, parallel mechanisms influencing the vaginal microbiota and pathogen colonization remain overlooked. In this study, we combine a mouse model of Streptococcus agalactiae strain COH1 (GBS) vaginal colonization with a mouse model of pubertal-onset obesity to assess diet as a determinant of vaginal microbiota composition and its role in colonization resistance. We leveraged culture-dependent assessment of GBS clearance and culture-independent, sequencing-based reconstruction of the vaginal microbiota in relation to diet, obesity, glucose tolerance, and microbial dynamics across time scales. Our findings demonstrate that excessive body weight gain and glucose intolerance are not associated with vaginal GBS density or timing of clearance. Diets high in fat and low in soluble fiber are associated with vaginal GBS persistence, and changes in vaginal microbiota structure and composition due to diet contribute to GBS clearance patterns in nonpregnant mice. These findings underscore a critical need for studies on diet as a key determinant of vaginal microbiota composition and its relevance to reproductive and perinatal outcomes.IMPORTANCEThis work sheds light on diet as a key determinant influencing the composition of vaginal microbiota and its involvement in group B Streptococcus (GBS) colonization in a mouse model. The study shows that dietary components, such as dietary fat and soluble fiber, impact GBS density and timing of clearance in the female reproductive tract. These findings are particularly significant given clear links between GBS and adverse reproductive and neonatal outcomes, advancing our understanding by identifying critical connections between dietary components, factors originating from the intestinal tract, vaginal microbiota, and reproductive outcomes.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Diet influences community dynamics following vaginal group B Streptococcus colonization

Megli,

DePuyt,

Goff

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Finally, the effective use of social media communication possibilities, for example to organize or to follow a scientific conference or a workshop, often saves time and valued financial resources. Twitter, for example, can be used to communicate the main points of a presentation at a scientific conference, [34] whereas some conferences (and particularly seminars) may be held fully online in social media platforms on LinkedIn, Facebook video, and YouTube platforms with synchronous Q&A [Question and Answer] session open to viewers.…”

Section: Resultsmentioning

confidence: 99%

Social Media for Chemistry Scholars**

Ciriminna

Scurria²,

Pagliaro

2023

ChemistryOpen

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Used by nearly 60 % of the world's population, social media are highly interactive websites (“platforms”) today widely used also by researchers. This perspective aims at identifying the main benefits of social media utilization by chemistry scholars with respect to the three main dimensions (research, education and societal service) of scholarly activity. The main risks arising from the use of social media, as we suggest in the conclusions, must be managed and new education on their purposeful utilization should be planned and delivered

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“…2 ). This finding suggests that host selective pressures drive the vaginal community towards a skewed dominance of a single organism independent of microbial exposure (23–25). We observed high variability between cohorts of HMb mice sampled at different times from the same colony, but this variability could still be grouped into consistent h mCSTs ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…are the most common members in C57BL/6J mice (23, 24), Enterobacteriaceae and Proteus spp. are dominant in CD-1 mice (25), and Streptococcus spp. and Proteus spp.…”

Section: Introductionmentioning

confidence: 99%

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

Mejia

Mercado-Evans

Zulk

et al. 2023

Preprint

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Vaginal microbiota composition is associated with differential risk of urogenital infection. Although vaginal Lactobacillus spp. are thought to confer protection through acidification, bacteriocin production, and immunomodulation, lack of an in vivo model system that closely resembles the human vaginal microbiota remains a prominent barrier to mechanistic discovery. We performed 16S rRNA amplicon sequencing of wildtype C57BL/6J mice, commonly used to study pathogen colonization, and found that the vaginal microbiome composition varies highly both within and between colonies from three distinct vivaria. Because of the strong influence of environmental exposure on vaginal microbiome composition, we assessed whether a humanized microbiota mouse (HMbmice) would model a more human-like vaginal microbiota. Similar to humans and conventional mice, HMbmice vaginal microbiota clustered into five community state types (hmCST). Uniquely, HMbmice vaginal communities were frequently dominated by Lactobacilli or Enterobacteriaceae. Compared to genetically-matched conventional mice, HMbmice were less susceptible to uterine ascension by urogenital pathobionts group B Streptococcus (GBS) and Prevotella bivia, but no differences were observed with uropathogenic E. coli. Specifically, vaginal Enterobacteriaceae and Lactobacillus were associated with the absence of uterine GBS. Anti-GBS activity of HMbmice vaginal E. coli and L. murinus isolates, representing Enterobacteriaceae and Lactobacillus respectively, were characterized in vitro and in vivo. Although L. murinus reduced GBS growth in vitro, vaginal pre-inoculation with HMbmouse-derived E. coli, but not L. murinus, conferred protection against vaginal GBS burden. Overall, the HMbmice are an improved model to elucidate the role of endogenous microbes in conferring protection against urogenital pathogens.

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Interrelated Effects of Zinc Deficiency and the Microbiome on Group B Streptococcal Vaginal Colonization

Cited by 9 publications

References 42 publications

Diet influences community dynamics following vaginal group B Streptococcus colonization

Diet influences community dynamics following vaginal group B Streptococcus colonization

Social Media for Chemistry Scholars**

Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model

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