Interrelation Between Luteal Cell Types in Steroidogenesis in Vitro of Human Corpus Luteum

MORI, TAKAHIDE; NIHNOBU, KENJI; TAKEUCHI, SATORU; ONHO, YOSHIO; Tojo, Shimpei

doi:10.1016/b978-0-08-030771-8.50116-9

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…) Discussion The separation of the thecal compartment from the inner granulosa lutein cells has provided new insights into their respective steroidogenic capacities in the rabbit corpus luteum. The results are in general agreement with those of Mori, Nihnobu, Takeuchi, Onho & Tojo (1983) who observed higher oestrogen and androgen formation from [14C]pregnenolone by the thecal compartment of the human corpus luteum, than by the granulosa lutein cells, although human granulosa lutein cells retain aromatizing activity. The production of oestradiol for several days by the thecal compart¬ ment (Fig.…”

Section: Luteal Tissue Content Ofprotein and Dnasupporting

confidence: 93%

Steroidogenic and morphological characteristics of granulosa and thecal compartments of the differentiating rabbit corpus luteum in culture

Yuh¹,

Possley²,

Brabec³

et al. 1986

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On the day after ovulation, the thecal tissue and associated mural granulosa lutein cells of the rabbit corpus luteum were separated from the granulosa lutein 'core' by dissection and these tissues were cultured separately or together (whole corpus luteum) in defined medium for 10 days on stainless-steel grids. The medium was changed completely every 24 h. Replicate tissues were cultured with testosterone (10 ng/ml), but no other hormones were added to the medium. Progesterone production increased during the first 2 days of culture for whole corpus luteum, granulosa lutein cells and the thecal compartment which also included granulosa lutein cells. After 3 days, the production of progesterone declined gradually, but was still detectable on Day 10. The production of the metabolite, 20 alpha-dihydroprogesterone, by whole corpus luteum was equal to or greater than that of progesterone. Without the addition of testosterone, the granulosa lutein cells produced little (10 pg/culture) oestradiol during 1 day of culture, but the thecal compartment and whole corpus luteum each produced about 100 pg/culture on Day 1 and declining quantities over the next 2 days. In the presence of testosterone added to the medium, the formation of oestradiol was greatly increased for all tissues for 5-6 days of culture, after which time oestradiol was no longer detectable with or without testosterone in medium. Transmission electron microscopy of cells after 10-12 days of culture revealed fine structure that is characteristic of luteal cells, including abundant smooth endoplasmic reticulum, lipid droplets, and junctions between the luteal cells. The corpus luteum in culture resembles the corpus luteum in situ in that steroidogenesis and differentiation can proceed for a period after ovulation without extrinsic hormonal stimulation.

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Section: Luteal Tissue Content Ofprotein and Dnasupporting

confidence: 93%

Steroidogenic and morphological characteristics of granulosa and thecal compartments of the differentiating rabbit corpus luteum in culture

Yuh¹,

Possley²,

Brabec³

et al. 1986

Reproduction

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“…Thus a 'two-cell' type mech¬ anism of oestradiol biosynthesis appears to operate in the human CL analogous to that in the preovulatory follicle. Histological studies show that theca-lutein cells become conspicuous at the same time that the human CL commences high rates of oestrogen secretion (Corner, 1956;Fujita, Mori, Suzuki et al 1981;Macnaughton, Kader, Gaukroger & Coutts, 1981;Mori, Nihnobu, Takeuchi et al 1983) and theca cells undertake de-novo androgen biosynthesis from cholesterol in vitro (Bjersing, 1981). Therefore, the rate of androgen biosynthesis in theca-lutein cells (hence aromatization in granulosa-lutein cells) is probably limited by the availability of extracellular steroidogenic precursors during the early luteal phase.…”

Section: Discussionmentioning

confidence: 97%

Cellular basis of luteal steroidogenesis in the human ovary

Fisch¹,

Margara²,

Winston³

et al. 1989

Journal of Endocrinology

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A primary monolayer cell culture system was developed to investigate human corpus luteum (CL) function in vitro. Steroidogenic cells were isolated by collagenase dispersal and Percoll density-gradient fractionation from CLs enucleated at progressive stages of the luteal phase (tubal surgery patients). 'Pure' granulosa-lutein cells were aspirated from ovulatory follicles at mid-cycle (in-vitro fertilization patients). The steroidogenic capacity (progesterone/20 alpha-dihydroprogesterone biosynthesis and aromatase activity) of isolated luteal cells was assessed in relation to CL development. Basal luteal cell steroidogenesis was maximal at around the expected time of ovulation and declined with CL age during the luteal phase. Conversely, human chorionic gonadotrophin (hCG)-responsive steroidogenesis was initially undetectable but developed as the luteal phase progressed. These results show that luteal cell steroidogenesis becomes increasingly dependent upon gonadotrophic support with CL age. This is evidence that functional luteolysis in human ovaries (1) is pre-programmed to occur at the cellular level, (2) is initiated automatically at the time of ovulation and (3) is reversed at the time of CL 'rescue' in early pregnancy by the direct action of trophoblastic hCG on steroidogenic luteal cells. The culture system described should be of value in further defining the control of human CL form and function at the cellular level.

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“…These findings also suggest that a "two-cell" model of estrogen syn thesis, similar to that proposed for the follicle, 24 is retained in the primate corpus luteum. Although the two-cell theory has not been tested in the corpus luteum, Mori et al 25 report that outer (presumably paraluteal cell-enriched) slices of the human corpus luteum produce more androgen than inner (true luteal cell-enriched) slices, whereas the latter is more po tent in progesterone and estrogen formation. Whether the paraluteal and luteal cells correspond to the small and large luteal cells isolated from corpora lutea of many species, including primates, is not clear.…”

Section: Luteal Cell Subpopulations: Morphology Size; and Functionmentioning

confidence: 99%

Cellular Approaches to Understanding the Function and Regulation of the Primate Corpus Luteum

Brannian

Stouffer²

1991

Semin Reprod Med

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Interrelation Between Luteal Cell Types in Steroidogenesis in Vitro of Human Corpus Luteum

Cited by 3 publications

References 14 publications

Steroidogenic and morphological characteristics of granulosa and thecal compartments of the differentiating rabbit corpus luteum in culture

Steroidogenic and morphological characteristics of granulosa and thecal compartments of the differentiating rabbit corpus luteum in culture

Cellular basis of luteal steroidogenesis in the human ovary

Cellular Approaches to Understanding the Function and Regulation of the Primate Corpus Luteum

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