Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

Reitermayer, Dominik; Kafka, Thomas A.; Lenz, Christian A.; Vogel, Rudi F.

doi:10.1186/s12866-018-1203-y

Cited by 59 publications

(38 citation statements)

References 58 publications

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“…Tween80 consists of polyethoxylated sorbitan and oleic acid. Oleic acid is the most abundant fatty acid found in animal fats and is often used as an additive in media to provide bacteria with oleic acid (Man et al, 2008; Reitermayer et al, 2018). In a subsequent experiment, influence of heme on bacterial growth was tested by addition of 2 μg/ml heminchloride (Roth, Karlsruhe, Germany) solved in dimethylsulfoxide (99.8%) (Roth, Karlsruhe, Germany) after autoclaving due to its light and heat instability.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Oxygen Consumption and Respiratory Activity of Meat Spoiling Bacteria Upon High Oxygen Modified Atmosphere

et al. 2019

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High oxygen modified atmosphere packaging is a commonly applied method to prolong the minimum shelf life of fresh (red) meats. Upon spoilage, changes of the initial oxygen concentration and microbiome composition can be observed. Thus, we classified the typical representative meat spoiling bacteria Brochothrix (B.) thermosphacta TMW2.2101 and the four lactic acid bacteria (LAB) Carnobacterium (C.) divergens TMW2.1577, C. maltaromaticum TMW2.1581, Leuconostoc (L.) gelidum subsp. gelidum TMW2.1618, and L. gelidum subsp. gasicomitatum TMW2.1619 along their oxygen consuming capacity, which can indicate the timeline of microbiome and sensorial changes. All bacteria were grown in a model system employing gas tight glass bottles containing meat simulation media and under modified atmosphere (70% O2 and 30% CO2). Oxygen concentrations of media and headspaces were monitored over time and the oxygen uptake rate (OUR) was calculated for all species. All bacteria were able to consume dissolved oxygen, with B. thermosphacta TMW2.2101 exhibiting a 31-times higher OUR per single cell in 60 h. Furthermore, all strains showed significant growth enhancement in the presence of heme indicating respiratory activity. Comparative genomic and physiological analyses predict the activity of a respiratory chain for all species upon high oxygen atmosphere. An additional cytochrome aa3 oxidase is suggested to be responsible for the increased OUR of B. thermosphacta TMW2.2101. Furthermore, B. thermosphacta TMW2.2101 revealed highest oxidative stress tolerance compared to the other bacteria, facilitating a higher respiratory activity. Coupling of respiration and fermentation via regeneration of NADH can be a competitive advantage for meat spoiling bacteria resulting in a higher cell count and possibly accelerated spoilage. The exhibited highest capacity for oxygen consumption of B. thermosphacta compared to LAB in vitro also suggests a higher contribution of this bacterium to the change in the atmosphere upon spoilage of MAP meats in situ.

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Section: Methodsmentioning

confidence: 99%

Quantitative Oxygen Consumption and Respiratory Activity of Meat Spoiling Bacteria Upon High Oxygen Modified Atmosphere

et al. 2019

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“…This component is known to support cell division of lactobacilli by modifying the cell membrane fatty acids composition (49). In addition, it induces a down-regulation of the de novo fatty acid synthesis that helps the cells to save intracellular energy (28).…”

Section: Effect Of Culture Conditions On Lactobacillus Reuteri Dsm 17938 Growth Performancesmentioning

confidence: 99%

“…Tween 80 is a key growth factor in culture media for lactobacilli, as it brings unsaturated fatty acids that allow reducing intracellular energy consumption, thanks to the down-regulation of de novo fatty acid synthesis in membrane phospholipids (28). It is for instance present at a concentration of 1 gꞏL -1 in MRS medium (12,29) or added to reach 5 gꞏL -1 (21).…”

Section: Introductionmentioning

confidence: 99%

Culture conditions affect Lactobacillus reuteri DSM 17938 ability to perform glycerol bioconversion into 3-hydroxypropionic acid

Nguyen

Saulou‐Bérion

Delettre

et al. 2021

Journal of Bioscience and Bioengineering

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The platform molecule 3-hydroxypropionic acid (3-HP) can be produced using Lactobacillus reuteri through a two-step bioprocess that involves a growth phase followed by a bioconversion phase. The bioproduction is performed by resting cells that convert glycerol into 3-HP and 1,3propanediol in fed-batch mode. This work aimed at studying the effect of the growth conditions of L. reuteri DSM 17938 during the first step, on the glycerol bioconversion into 3-HP during the second step. A Plackett and Burman design was carried out to test, in controlled bioreactors, the effect of 11 growth conditions simultaneously, at fixed bioconversion conditions. The supplementation of the growth medium with vitamin B12 and cysteine displayed a negative effect on the 3-HP bioproduction.

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“…Polysorbates increased the biomass production of strain RPR42 in various formula of cane molasses-based media. Such positive effects of polysorbates on the growth of lactobacilli seems to be associated to the fact that these fastidious firmicutes can utilize the fatty acid moieties of polysorbates in the biosynthetic pathways (Reitermayer et al 2018). Furthermore, the effects of polysorbates on the growth of strain RPR42 were more detectable in cane molasses-based media containing CSL and WGE than cheese whey (Additional file 1: Figures S5, S7, and S9).…”

Section: Discussionmentioning

confidence: 99%

Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

et al. 2020

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Nitrogen source has a vital role for the efficient growth of lactobacilli. The effects of cheese whey, corn steep liquor, and wheat germ extract on the growth of L. plantarum strain RPR42 in cane molasses-based media was evaluated using various approaches of design of experiments. Our results showed that such protein-rich agricultural by-products significantly increase the biomass production of the strain RPR42 in cane molasses-based media. The most affecting nitrogenous material was cheese whey followed by CSL and the minor effect was reported for wheat germ extract as revealed in factorial and Box-Behnken design experiments. The replacement of costly beef extract and yeast extract with a defined mixtures of the above nitrogenous agricultural by-products in cane molasses-based medium led to production of up to 12.64 g/L/24 h of dry biomass of strain RPR42. A detectable cell density of strain RPR42 (~ 9.81 × 10 9 CFU/mL 24 h) which was observed in such an economic medium showed that the large-scale production of the strain RPR42 tend to be feasible at significantly low costs.

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Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

Cited by 59 publications

References 58 publications

Quantitative Oxygen Consumption and Respiratory Activity of Meat Spoiling Bacteria Upon High Oxygen Modified Atmosphere

Quantitative Oxygen Consumption and Respiratory Activity of Meat Spoiling Bacteria Upon High Oxygen Modified Atmosphere

Culture conditions affect Lactobacillus reuteri DSM 17938 ability to perform glycerol bioconversion into 3-hydroxypropionic acid

Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

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