Abstract. Four soybean g-notypes (Glycine mnax L. Merrill) differing in their ability to accumu!ate iron were studied: the efficient genotypes Hawkeye (HA) and A62-9 (E-9) and the inefficient g-notypes PI-54619-5-1 (PI) ar.d A62-10 (1-10).The distr.bution of iron in the tissues of plants grown in a growth chamber in nutrient solutions containing various levels of iron was determined. A greater amount of iron was associat-d with the roots of inefficient plants than with roots of efficient plants, indicating a slower rate of iron translocation in the former. After determination of the amount of iron in the shoots at low levels of nutrient iron, the a.bility of the sevcral genotypes to accumulate iron was rated HA> E-9> 1-10.> PI. At the highest level of nutrient iron the rated efficiencies were E-9> HA> 1-10> PI. Accumulation of iron in the primary leaves provided an excellent indication of whole-plant iron accumulation. A reduction in accumulation of iron by efficient plants occurred when the plants were grown together in the same solution as inefficient ones.Weiss (23) found that the inability of a number of soybean varieties from Manchuria to ob.ain sufficient iron was governed by a single gene. Brown et al. (13) crossed one of these in-efficient varieties, PI-54619-5-1 (P1), wiLh an efficient American variety, Hawkeye (HA), and selected the efficient isoline A62-9 (E-9) and the inefficient isoline AV62-10 (I-10). The isogenic lines provide an excellent source of material for studying the influence of genes on higher plant nutrition andI may be useful in resolving mechanisms involved in iron nutrition. Dif