Interrelationships Between miR-34a and FSH in the Control of Porcine Ovarian Cell Functions

Fabová, Zuzana; Loncová, Barbora; Bauer, Miroslav; Sirotkin, Alexander V.

doi:10.1007/s43032-022-01127-2

Cited by 2 publications

(2 citation statements)

References 48 publications

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“…MicroRNA 34a, as a member of the miR-34 family (miR-34a/b/c, miR-34s) [45], has been proposed to control essential ovarian functions and negatively regulate the local translation of FSHR. In porcine ovarian cells, FSH promotes the expression of miR-34a [46], indicating that miR-34a may be involved in controlling ovarian functions; as both, miR-34a and FSH are synergists in their actions on follicular cell activity. Therefore, the influence of miR-34a on FSHR may depend on FSH levels which are high during canine anestrus.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs 34a and -let-7c in Canine Follicles during the Estrous Cycle

De los Reyes,

Dettleff,

Palomino

et al. 2024

Animals

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The genes encoding for estrogen receptor (ESR2) and follicle-stimulating hormone receptor (FSHR) play crucial roles in ovarian follicular development. This study aimed to determine the expression levels of miRNAs predicted against FSHR and ESR2 mRNAs in follicular cells related to their target genes during the estrous cycle in canines. Antral follicles were dissected from 72 ovaries following ovariohysterectomies. MiRNAs regulating FSHR and ESR2 genes were selected from miRNA databases, and mature miRNA and mRNA expression profiling was performed using real-time polymerase chain reaction (PCR). The best miRNA for each target gene was selected considering the quantitative PCR (qPCR) performance and target prediction probability, selecting only miRNAs with a binding p-value of 1.0, and choosing cfa-miR-34a and cfa-let-7c for FSHR and ESR2, respectively. The expression levels comparing the different phases of the estrous cycle were evaluated using ANOVA. Pearson correlations between the expression pattern of each miRNA and their target genes were performed. Each miRNA and its target genes were expressed in the granulosa cells in all estrous phases. FSHR remained low in anestrus and proestrus, increased (p < 0.05) to the highest level in estrus, and decreased (p < 0.05) in diestrus. ESR2 showed the same trend as FSHR, with the highest (p < 0.05) expression in estrus and the lowest (p < 0.05) in anestrus and proestrus. A tendency for an inverse relationship was observed between the expression of miR-34a and FSHR only in the anestrus phase, while an inverse correlation (r = −0.8) was found between miRNA-7c and ESR2 (p < 0.01). The expression profile of miR-34a and miR-let-7c and their predicted target genes of dog ovarian follicles throughout the estrous cycle observed in this study suggest a role in the transcriptional regulation of FSHR and ESR2, which is the first evidence of the involvement of these miRNAs in the canine follicular function.

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Section: Discussionmentioning

confidence: 99%

Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs 34a and -let-7c in Canine Follicles during the Estrous Cycle

De los Reyes,

Dettleff,

Palomino

et al. 2024

Animals

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“…Inclusion criteria of participants comprised: (1) females aged 35 to 50 years, (2) BMI > 25 kg/m 2 , no history of CVDs or other diseases such as metabolic disorders, diabetes mellitus, hypertension, thyroid abnormalities, fatty liver disease, pulmonary disease, musculoskeletal disease, gastrointestinal disease, polycystic syndrome, and autoimmune and neurologic diseases, (3) no history of regular exercise training, dietary regimen for body mass loss or mass gain, pharmacological and hormonal interventions and smoking for at least 6 months before the start of the study, and (4) being in the 8th to the 16th day of their menstrual cycle. Numerous studies indicate that changes in miRNAs, such as miR-155, miR-145, and miR-34a, are related to changes in the levels of estrogen and progesterone hormones [24][25][26]. We attempted to enroll participants who were in similar menstrual phases/periods to eliminate the potential effects of sex hormones changes on miRNAs.…”

Section: Participantsmentioning

confidence: 99%

Effects of HIIT training and HIIT combined with circuit resistance training on measures of physical fitness, miRNA expression, and metabolic risk factors in overweight/obese middle-aged women

Pashaei,

Malandish,

Alipour

et al. 2024

BMC Sports Sci Med Rehabil

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Objective The purpose of this study was to examine the effects of 10 weeks of high-intensity interval training (HIIT) and HIIT combined with circuit resistance training (HCRT) on selected measures of physical fitness, the expression of miR-9, -15a, -34a, -145, and − 155 as well as metabolic risk factors including lipid profiles and insulin resistance in middle-aged overweight/obese women. Methods Twenty-seven overweight/obese women aged 35–50 yrs. were randomized to HIIT (n = 14) or HCRT (n = 13) groups. The HIIT group performed running exercises (5 reps x 4 min per session) with active recovery between repetitions for 10 weeks with 5 weekly sessions. The HCRT group performed 10 weeks of HIIT and resistance training with 3 weekly HIIT sessions and 2 weekly HCRT sessions. Anthropometric measures (e.g., body mass), selected components of physical fitness (cardiovascular fitness, muscle strength), levels of miRNAs (miR-9, -15a, -34a, -145, and − 155), lipid profiles (total cholesterol; TC, Triglycerides; TG, low-density lipoprotein cholesterol; LDL-C and high-density lipoprotein cholesterol; HDL-C), and insulin resistance; HOMA-IR index, were measured at baseline and week 10. Results An ANOVA analysis indicated no significant group by time interactions (p > 0.05) for all anthropometric measures, and maximum oxygen consumption (VO2max). A significant group by time interaction, however, was found for the one-repetition maximum (IRM; p < 0.001, ES= 0.751 , moderate). A post-hoc test indicated an increase in the pre-to-post mean 1RM for HCRT (p = 0.001, ES = 1.83, large). There was a significant group by time interaction for miR-155 (p = 0.05, ES = 0.014, trivial). Levels for miR-155 underwent pre-to-post HIIT increases (p = 0.045, ES = 1.232, large). Moreover, there were also significant group by time interactions for TC (p = 0.035, ES = 0.187, trivial), TG (p < 0.001, ES = 0.586, small), LDL-C (p = 0.029, ES = 0.200, small) and HDL-C (p = 0.009, ES = 0.273, small). Post-hoc tests indicated pre-post HCRT decreases for TC (p = 0.001, ES = 1.44, large) and HDL-C (p = 0.001, ES = 1.407, large). HIIT caused pre-to-post decreases in TG (p = 0.001, ES = 0.599, small), and LDL-C (p = 0.001, ES = 0.926, moderate). Conclusions Both training regimes did not improve cardiovascular fitness. But, HCRT improved lower/upper limb muscle strength, and HIIT resulted in an increase in miR-155 expression in peripheral blood mononuclear cells. Furthermore, HIIT and HCRT each improved selected metabolic risk factors including lipid profiles and glucose and insulin metabolism in overweight/obese middle-aged women. Trial registration OSF, October, 4th 2023. Registration DOI: https://doi.org/10.17605/OSF.IO/UZ92E. osf.io/tc5ky. “Retrospectively registered”.

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Interrelationships Between miR-34a and FSH in the Control of Porcine Ovarian Cell Functions

Cited by 2 publications

References 48 publications

Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs 34a and -let-7c in Canine Follicles during the Estrous Cycle

Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs 34a and -let-7c in Canine Follicles during the Estrous Cycle

Effects of HIIT training and HIIT combined with circuit resistance training on measures of physical fitness, miRNA expression, and metabolic risk factors in overweight/obese middle-aged women

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