Interrelationships of flow, intravascular pressure, and tissue perfusion in the measurement of capillary permeability to sodium in isolated dog lung lobes.

Tancredi, Robert G.; Yipintsoi, Tada

doi:10.1161/01.res.46.5.669

Cited by 11 publications

(1 citation statement)

References 35 publications

(53 reference statements)

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“…The eel rete arterial capillaries are much thicker than continuous non-neural mammalian capillaries, but they contain an approximately equivalent density of vesicles (Rasio et al, 1981). The rete permeability values approximate the same order of magnitude as those estimated by tracer dilution for isolated dog lungs perfused by high rates of plasma flow (Tancredi et al, 1980); and the rete values, in turn, are approximately 10 times higher than the permeability values of brain capillaries, measured with sensitive microelectrodes placed in the interstitium (Crone, 1975;Hansen et al, 1977).…”

Section: Comparison With Other Valuessupporting

confidence: 55%

Passage of ions and dextran molecules across the rete mirabile of the eel. The effects of charge.

Rasio¹,

Goresky²

1985

Circulation Research

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SUMMARY. The countercurrent-perfused rete mirabile of the eel is a preparation in which capillary permeability values can be determined accurately in broadscale fashion. To provide insight into charge effects on transendothelial passage, the permeability values of small inorganic cations (labeled sodium and rubidium, and stable potassium) and anions (labeled chloride, iodide, sulfate, and ferrocyanide) were compared to those expected for neutral solutes with approximately matched diffusion coefficients, and that of a neutral dextran fraction was compared to that of a negatively charged dextran sulfate with a similar diffusion coefficient. In the small ion experiments, the labeled iodide values were unexpectedly high, apparently due to the contamination of the labeled iodide solution with IJ~. The permeabilities of the rest of the ions clustered at a level about 0.5 of the values which would have been expected for neutral solutes with similar diffusion coefficients. The decrement was interpreted to reflect the presence, of both positive and negative charges along the transendothelial pathway, which effectively decrease the dimensions of the limiting part of the pathway for the charged microions relative to that accessible to comparable nonelectrolytes. The larger negatively charged dextran sulfate was also reduced in its passage, in comparison with its matching neutral dextran; this was taken to indicate the presence of a larger1 scale average net negative charge along its pathway. The data indicate the presence of a staggering of positive and negative charges along the transendothelial pathway accessible to the microions, and a net negative charge in the more restricted part of the pathway available to the dextrans. (CircRes 56: 74-83, 1985) BLOOD capillary permeability to small ions has long been known to be a minute fraction of that for water, irrespective of the methods used and tissues investigated. The data have been interpreted as indicating the presence of differing paths for the passage of ions and water across the endothelial wall, the mechanism for the transfer of each usually being thought to be passive [except in certain special areas, such as the tight capillaries of the central nervous system and the eye, where evidence for some active transport of sodium and potassium ions has been presented (Bradbury and Stulcova, 1970;Eisenberg and Suddith, 1979;Betz and Goldstein, 1980)]. It has been expected that appropriately designed studies with ions and charged molecules would provide information concerning the effects of local fixed charges, if these are present in the regions where inorganic ions and other charged materials traverse the capillary wall.Few capillary beds have been thoroughly examined from this point of view. The glomerular apparatus of the kidney constitutes the one exception. Studies of glomerular filtration of moderately large molecules with different charges and comparable molecular size have provided evidence for a charge effect in this structure. These studies indicate that, altho...

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Section: Comparison With Other Valuessupporting

confidence: 55%

Passage of ions and dextran molecules across the rete mirabile of the eel. The effects of charge.

Rasio¹,

Goresky²

1985

Circulation Research

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Pulmonary Oedema

Chinard¹

1987

Advances in Physiological Research

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An interpretation of14C-urea and14C-primidone extraction in isolated rabbit lungs

et al. 1996

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We measured the venous concentration versus time curves of 14C-urea and 14C-primidone after rapid bolus injections of a vascular reference indicator, fluorescein isothiocyanate dextran, and one of the two 14C-labeled indicators in isolated rabbit lungs perfused with Krebs-Ringer bicarbonate solution containing 4.5% bovine serum albumin at flow rates (F) of 6.67, 3.33, 1.67, and 0.83 ml/sec and with nearly constant microvascular pressure and total lung vascular volume. When we calculated the permeability-surface area product, PS, from the 14C-urea and 14C-primidone outflow curves using the Crone model, the estimates of the PS product were directly proportional to F. However, the fractional change in the PS with flow was different for the two indicators. We also estimated the PS from the same 14C-urea and 14C-primidone data using an alternative model that includes perfusion heterogeneity, estimated in a previous study, and flow-limited and barrier-limited extravascular volumes accessible to both urea and primidone. This model was able to fit the outflow curves of either 14C-urea or 14C-primidone at all four flows studied with one flow-independent PS for each indicator. The ability of the new model to explain the 14C-urea and 14C-primidone data with no flow-dependent change in PS suggests that a change in PS with F estimated using other models such as the Crone model is not sufficient for capillary surface area recruitment.

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Interrelationships of flow, intravascular pressure, and tissue perfusion in the measurement of capillary permeability to sodium in isolated dog lung lobes.

Abstract: SUMMARY From venous tracer-dilution curves, after 57 pulse injections of "NaCl and [ 13I

Cited by 11 publications

References 35 publications

Passage of ions and dextran molecules across the rete mirabile of the eel. The effects of charge.

Passage of ions and dextran molecules across the rete mirabile of the eel. The effects of charge.

Pulmonary Oedema

An interpretation of14C-urea and14C-primidone extraction in isolated rabbit lungs

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