Intersex and reproductive impairment of wild fish exposed to multiple municipal wastewater discharges

Tetreault, Gerald R.; Bennett, Charles H.; Shires, Kallie; Knight, Brendan W.; Servos, Mark R.; McMaster, Mark E.

doi:10.1016/j.aquatox.2011.05.008

Cited by 198 publications

(157 citation statements)

References 47 publications

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“…Individual fish were assessed for sex, total length (AE 1 mm), and total weight (AE 0.01 g). Condition factor, the ratio of body weight to total length, was calculated as body weight Â 100/length 3 . Prior to decapitation of the Murray rainbowfish, blood was collected from the caudal vein using heparinized capillary tubes.…”

Section: Fish Sampling and Endocrine Disruption Biomarker Analysismentioning

confidence: 99%

Integrated assessment of wastewater treatment plant effluent estrogenicity in the Upper Murray River, Australia, using the native Murray rainbowfish (Melanotaenia fluviatilis)

Vajda

Kumar

Woods

et al. 2015

Enviro Toxic and Chemistry

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The contamination of major continental river systems by endocrine-active chemicals (EACs) derived from the discharge of wastewater treatment plant (WWTP) effluents can affect human and ecosystem health. As part of a long-term effort to develop a native fish model organism for assessment of endocrine disruption in Australia's largest watershed, the Murray-Darling River Basin, the present study evaluated endocrine disruption in adult males of the native Australian Murray rainbowfish (Melanotaenia fluviatilis) exposed to effluent from an activated sludge WWTP and water from the Murray River during a 28-d, continuous-flow, on-site experiment. Analysis of the WWTP effluent and river water detected estrone and 17β-estradiol at concentrations up to approximately 25 ng L(-1) . Anti-estrogenicity of effluent samples was detected in vitro using yeast-based bioassays (yeast estrogen screen) throughout the experiment, but estrogenicity was limited to the first week of the experiment. Histological evaluation of the testes indicated significant suppression of spermatogenesis by WWTP effluent after 28 d of exposure. Plasma vitellogenin concentrations and expression of vitellogenin messenger RNA in liver were not significantly affected by exposure to WWTP effluent. The combination of low contaminant concentrations in the WWTP effluent, limited endocrine disrupting effects in the Murray rainbowfish, and high in-stream dilution factors (>99%) suggest minimal endocrine disruption impacts on native Australian fish in the Murray River downstream from the WWTP outfall.

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Section: Fish Sampling and Endocrine Disruption Biomarker Analysismentioning

confidence: 99%

Integrated assessment of wastewater treatment plant effluent estrogenicity in the Upper Murray River, Australia, using the native Murray rainbowfish (Melanotaenia fluviatilis)

Vajda

Kumar

Woods

et al. 2015

Enviro Toxic and Chemistry

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“…Rainbow darter (RBD) is a native small bodied, benthic fish found throughout central North America, and is also widespread in the Grand River, Ontario, Canada. Intersex in RBD has been reported in recent years in the Grand River (Tetreault et al, 2011;Tanna et al, 2013;Bahamonde et al, 2014), making it a sensitive and relevant model for studying intersex in fish. Therefore, one of the aims of this research was to develop a more standardized approach to assessing the intersex condition as an endpoint for potential use in monitoring programs for small bodied fish.…”

Section: Introductionmentioning

confidence: 99%

Whole organism responses and intersex severity in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part A

et al. 2015

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“…As most of these substances are designed to be biologically active, they can affect sensitive aquatic organisms even at very low concentrations (Santos et al, 2010), hence the name "micropollutant". For instance, endocrine effects on fish and mussel populations such as intersex, reproductive disruption or feminization of males have been observed in rivers downstream of municipal WWTP outfalls (Alan et al, 2008;Gagné et al, 2011;Tetreault et al, 2011;Tyler and Jobling, 2008;Vethaak et al, 2005;Woodling et al, 2006). These effects were attributed to the release of endocrine-active chemicals such as the synthetic estrogen 17α-ethinylestradiol (found in contraceptive pills), natural estrogens estrone and 17β-estradiol or nonylphenol.…”

Section: Introductionmentioning

confidence: 99%

Treatment of micropollutants in municipal wastewater: Ozone or powdered activated carbon?

Margot

Kienle

Magnet³

et al. 2013

Science of The Total Environment

684

404

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Highlights• Micropollutants are efficiently removed by both ozone and powdered activated carbon• Specific substances were removed more efficiently by ozone• Powdered activated carbon effectively removed a wider range of pollutants• Both treatments significantly reduced the toxicity of WWTP effluent AbstractMany organic micropollutants present in wastewater, such as pharmaceuticals and pesticides, are poorly removed in conventional wastewater treatment plants (WWTPs). To reduce the release of these substances into the aquatic environment, advanced wastewater treatments are necessary. In this context, two large-scale pilot advanced treatments were tested in parallel over more than one year at the municipal WWTP of Lausanne, Switzerland. The treatments were: i) oxidation by ozone followed by sand filtration (SF) and ii) powdered activated carbon (PAC) adsorption followed by either ultrafiltration (UF) or sand filtration. More than 70 potentially problematic substances (pharmaceuticals, pesticides, endocrine disruptors, drugs metabolites and other common chemicals) were regularly measured at different stages of treatment. Additionally, several ecotoxicological tests such as the yeast estrogen screen, a combined algae bioassay and a fish early life stage test were performed to evaluate effluent toxicity. Both treatments significantly improved the effluent quality. Micropollutants were removed on average over 80% compared with raw wastewater, with an average ozone dose of 5.7 mg O 3 l -1 or a PAC dose between 10 and 20 mg l -1 . Depending on the chemical properties of the substances (presence of electron-rich moieties, charge and hydrophobicity), either ozone or PAC performed better. Both advanced treatments led to a clear reduction in toxicity of the effluents, with PAC-UF performing slightly better overall. As both treatments had, on average, relatively similar efficiency, further criteria relevant to their implementation were considered, including local constraints (e.g., safety, sludge disposal, disinfection), operational feasibility and cost. For sensitive receiving waters (drinking water resources or recreational waters), the PAC-UF treatment, despite its current higher cost, was considered to be the most suitable option, enabling good removal of most micropollutants and macropollutants without forming problematic by-products, the strongest decrease in toxicity and a total disinfection of the effluent.

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Intersex and reproductive impairment of wild fish exposed to multiple municipal wastewater discharges

Cited by 198 publications

References 47 publications

Integrated assessment of wastewater treatment plant effluent estrogenicity in the Upper Murray River, Australia, using the native Murray rainbowfish (Melanotaenia fluviatilis)

Integrated assessment of wastewater treatment plant effluent estrogenicity in the Upper Murray River, Australia, using the native Murray rainbowfish (Melanotaenia fluviatilis)

Whole organism responses and intersex severity in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part A

Treatment of micropollutants in municipal wastewater: Ozone or powdered activated carbon?

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