Interspecies Nuclear Transfer: Implications for Embryonic Stem Cell Biology

Beyhan, Zeki; Iager, Amy E.; Cibelli, José B.

doi:10.1016/j.stem.2007.10.009

Cited by 95 publications

(91 citation statements)

References 88 publications

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“…To our knowledge, Ch embryos have not previously been produced by cloning, and this species can be used as a model of the big wild felids from different genera than the Dc (Johnson et al 2006). This was a big challenge considering that the ability of an interspecific embryo to develop to the blastocyst stage decreases as the taxonomic distance between donor and recipient species increases (Beyhan et al 2007). Moreover, the present work is the first to report embryo aggregation as a strategy to improve cloning efficiency in felids.…”

Section: Discussionmentioning

confidence: 75%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. Dc oocytes were in vitro matured and subjected to zona pellucida free (ZP-free) SCNT or iSCNT, depending on whether the nucleus donor cell was of Dc or Ch respectively. ZP-free reconstructed embryos were then cultured in microwells individually (Dc1X and Ch1X groups) or in couples (Dc2X and Ch2X groups). Embryo aggregation improved in vitro development obtaining 27.4, 47.7, 16.7 and 28.3% of blastocyst rates in the Dc1X, Dc2X, Ch1X and Ch2X groups, respectively (P!0.05). Moreover, aggregation improved the morphological quality of blastocysts from the Dc2X over the Dc1X group. Gene expression analysis revealed that Ch1X and Ch2X blastocysts had significantly lower relative expression of OCT4, CDX2 and NANOG than the Dc1X, Dc2X and IVF control groups. The OCT4, NANOG, SOX2 and CDX2 genes were overexpressed in Dc1X blastocysts, but the relative expression of these four genes decreased in the Dc2X, reaching similar relative levels to those of Dc IVF blastocysts. In conclusion, Ch blastocysts were produced using Dc oocytes, but with lower relative expression of pluripotent and trophoblastic genes, indicating that nuclear reprogramming could be still incomplete. Despite this, embryo aggregation improved the development of Ch and Dc embryos, and normalized Dc gene expression, which suggests that this strategy could improve full-term developmental efficiency of cat and feline iSCNT embryos.Reproduction (2015) 150 1-10

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Section: Discussionmentioning

confidence: 75%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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“…Subsequently, nuclear transfer has been well established with mouse models [7,24]. The nucleus of a somatic donor cells is isolated and transferred into an unfertilized, enucleated egg [148].…”

Section: Nuclear Transfermentioning

confidence: 99%

Developmental and Functional Nature of Human iPSC Derived Motoneurons

Stockmann

Linta

Föhr

et al. 2011

Stem Cell Rev and Rep

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“…This enthusiasm is often rejuvenated by research developments including the emergence of technologies such as induced pluripotent stem (iPS) cells [16], interspecies somatic cell nuclear transfer [17], parthenogenetic embryonic stem cells [18], and embryonic stem cells derived from single cells of the embryo [19]. While the majority of these technologies do not involve human embryos and may have distinct scientific purposes and advantages, they are nonetheless commonly linked with hESC research in the prevalent discourse.…”

Section: Introductionmentioning

confidence: 99%

Emerging Ethical, Legal and Social Issues Associated with Stem Cell Research & and the Current Role of the Moral Status of the Embryo

Zarzeczny

Caulfield

2009

Stem Cell Rev and Rep

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Since its early days, stem cell research, particularly human embryonic stem cell research, has been the focus of intense social debate, and the question of the moral status of the embryo has been a central issue in the controversy. Despite this friction, and while it has yet to obtain widespread success in clinical applications, stem cell research remains a great hope for future advances in healthcare. In this paper, we will discuss the results of our systematic literature review in which we examined recent social science, legal and biomedical discourse, as well as Canadian print media discourse, associated with stem cell research in order to assess the role the question of the moral status of the embryo currently plays in these forums, and to identify what other issues are emerging and receiving attention. This analysis will assist with recognizing the issues which are likely to inform future policy and will facilitate forecasting the probable direction of the continually developing social discourse surrounding stem cell research.

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Interspecies Nuclear Transfer: Implications for Embryonic Stem Cell Biology

Cited by 95 publications

References 88 publications

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Developmental and Functional Nature of Human iPSC Derived Motoneurons

Emerging Ethical, Legal and Social Issues Associated with Stem Cell Research & and the Current Role of the Moral Status of the Embryo

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