Interspecific and intraspecific transference of metabolism‐based mesotrione resistance in dioecious weedy <i>Amaranthus</i>

Oliveira, Maxwel C.; Gaines, Todd A.; Patterson, Eric; Jhala, Amit J.; Irmak, Suat; Amundsen, Keenan; Knežević, Stevan Z.

doi:10.1111/tpj.14089

Cited by 30 publications

(29 citation statements)

References 68 publications

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“…Wind speed at tassel height was correlated with frequency of gene flow, but only to a low degree. Studies with greater correlation of wind parameters with frequency of gene flow in waterhemp and giant ragweed have been in bare ground areas and report higher wind speeds (Ganie & Jhala, 2017; Sarangi et al., 2017), whereas, with a crop canopy present, wind speed tends to be slowed down (Baldocchi, Verma, & Rosenberg, 1983; Oliveira et al., 2018). Baltazar et al.…”

Section: Discussionmentioning

confidence: 99%

Risk assessment of pollen‐mediated gene flow from Ga1‐m field corn to dent‐sterile Ga1‐s popcorn

Barnes

Knežević

et al. 2020

Crop Science

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The popcorn industry in the United States is at risk of genetic contamination because it utilizes the gametophyte factor 1 gene (Ga1) as a barrier against pollen‐mediated gene flow (PMGF) from field corn (Zea mays L.). Popcorn with the Ga1‐s allele accepts pollen only from Ga1‐s corn, allowing for field corn and popcorn to be nearby without isolation. Germplasm is being introduced to the United States to increase field corn diversity that unknowingly contains the Ga1‐m allele, which can overcome Ga1‐s selectivity and pollinate popcorn. The risk to the popcorn industry has been underassessed. Experiments were conducted to model the frequency of PMGF from Ga1‐m field corn to Ga1‐s popcorn under field conditions and to evaluate the role of wind speed and direction using a concentric donor–receptor design in 2017 and 2018 in Nebraska, USA. The PMGF to white popcorn was detected using a field corn pollen donor with yellow kernel color (dominate) and further confirmed with a molecular test. Popcorn kernels were harvested from cardinal and ordinal directions from 1 to 70 m. More than 7 million kernels were screened to detect the PMGF. Information‐theoretic criteria were used to select the best‐fit model. The greatest PMGF (1.6– 4.1%) was detected at 1 m and declined with distance. The PMGF was detected at 70 m, the maximum distance tested. Amplification of Ga1 followed by restriction enzyme digest confirmed that yellow kernelled progeny were hybrids from the result of PMGF. This is the first assessment of PMGF from field corn to popcorn, and the results are alarming for the popcorn industry and export market.

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Section: Discussionmentioning

confidence: 99%

Risk assessment of pollen‐mediated gene flow from Ga1‐m field corn to dent‐sterile Ga1‐s popcorn

Barnes

Knežević

et al. 2020

Crop Science

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“…www.nature.com/scientificreports www.nature.com/scientificreports/ herbicide resistance alleles could already be present within the standing genetic variation of the Amaranthus spp. populations tested herein 55,56 . This could explain the differences in herbicide sensitivity shift between waterhemp and Palmer amaranth, and even the differences among populations (different genetic background) following recurrent selection with herbicide drift.…”

Section: Populationmentioning

confidence: 99%

Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Vieira

Luck

Amundsen

et al. 2020

Sci Rep

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While the introduction of herbicide tolerant crops provided growers new options to manage weeds, the widespread adoption of these herbicides increased the risk for herbicide spray drift to surrounding vegetation. the impact of herbicide drift in sensitive crops is extensively investigated, whereas scarce information is available on the consequences of herbicide drift in non-target plants. Weeds are often abundant in field margins and ditches surrounding agricultural landscapes. Repeated herbicide drift exposure to weeds could be detrimental to long-term management as numerous weeds evolved herbicide resistance following recurrent-selection with low herbicide rates. the objective of this study was to evaluate if glyphosate, 2,4-D, and dicamba spray drift could select Amaranthus spp. biotypes with reduced herbicide sensitivity. palmer amaranth and waterhemp populations were recurrently exposed to herbicide drift in a wind tunnel study over two generations. Seeds from survival plants were used for the subsequent rounds of herbicide drift exposure. progenies were subjected to herbicide doseresponse studies following drift selection. Herbicide drift exposure rapidly selected for Amaranthus spp. biotypes with reduced herbicide sensitivity over two generations. Weed management programs should consider strategies to mitigate near-field spray drift and suppress the establishment of resistance-prone weeds on field borders and ditches in agricultural landscapes.Some researchers also suggest that low rates of herbicides could induce new stress-related mutations and epigenetic alterations on weeds, ultimately leading to reduced herbicide sensitivity [33][34][35] .Recombination and accumulation of minor resistance alleles can occur at a faster rate in cross-pollinated species, such as Palmer amaranth and waterhemp, during recurrent selection with low rates of herbicides 21,22,36 . These C4 summer annual Amaranthus spp. are among the most troublesome weed species in the US row crop production systems 37 . Both are obligate outcrossing dioecious weed species with a fast growth habitat, extended emergence window, and prolific seed production with high genetic plasticity which pose a challenge to their management 37-44 . Numerous Palmer amaranth and waterhemp populations have evolved resistance to herbicides that target 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), 4-hydroxyphenylpyruvate dioxygenase (HPPD), photosystem II, protoporphyrinogen oxidase (PPO), auxin receptors, microtubule assembly, and acetolacte synthase (ALS) in the US 15,17,[45][46][47][48][49][50][51][52][53][54] . Moreover, pollen mediated gene flow has been reported as a major contributor to herbicide resistance dissemination in Palmer amaranth and waterhemp in the US Midwest 55,56 .Although controlling weed populations on field margins and ditches is considered a best management practice to delay herbicide resistance evolution, these weed populations are often neglected in agricultural landscapes [15][16][17]29 . The hypothesis of this study is that rep...

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“…The HEX and FAM fluorescence data for the individual samples were transformed into the percentage of relative fluorescence units (RFUs) for each fluorophore using the equation (Oliveira et al 2018):…”

Section: Discussionmentioning

confidence: 99%

Protoporphyrinogen oxidase (PPO) inhibitor–resistant waterhemp (Amaranthus tuberculatus) from Nebraska is multiple herbicide resistant: confirmation, mechanism of resistance, and management

et al. 2019

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A waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] biotype (designated as “NER”) collected from a soybean [Glycine max (L.) Merr.] production field in eastern Nebraska survived the POST application of fomesafen at the labeled rate. The objectives of this study were to (1) quantify the level of resistance to protoporphyrinogen oxidase (PPO) inhibitors (acifluorfen, fomesafen, and lactofen) applied POST, (2) determine the mechanism of PPO-inhibitor resistance in the NER biotype, (3) determine whether NER possessed multiple resistance to acetolactate synthase (ALS)-, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-, and photosystem II (PSII)-inhibiting herbicides, and (4) control NER with POST soybean herbicides. A whole-plant dose–response bioassay revealed that the NER biotype was 4- to 6-fold resistant to PPO-inhibiting herbicides depending on the known susceptible biotype (S1 or S2) used for comparison. A Kompetitive Allele Specific PCR (KASP™) assay was developed and performed for rapid and robust detection of the ΔG210 mutation (deletion of a codon) in the PPX2L gene. All samples of the NER biotype tested positive for the ΔG210 mutation. Dose–response bioassays confirmed that the NER biotype was resistant to three additional herbicide sites of action. Chlorimuron and imazethapyr, both ALS inhibitors, applied at 32X the labeled rate resulted in <80% reduction in the aboveground biomass of the NER biotype. The same biotype was 3- and 7-fold resistant to glyphosate (EPSPS inhibitor) and atrazine (PSII inhibitor), respectively. Glufosinate, 2,4-D choline plus glyphosate, and dicamba were the only soybean POST herbicides that controlled NER effectively (≥92% aboveground biomass reduction). Amaranthus tuberculatus is the first confirmed weed species in Nebraska to evolve resistance to four distinct herbicide sites of action, leaving no POST herbicide choice for effective control in glyphosate-resistant and conventional (non-transgenic) soybean.

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Interspecific and intraspecific transference of metabolism‐based mesotrione resistance in dioecious weedy Amaranthus

Cited by 30 publications

References 68 publications

Risk assessment of pollen‐mediated gene flow from Ga1‐m field corn to dent‐sterile Ga1‐s popcorn

Risk assessment of pollen‐mediated gene flow from Ga1‐m field corn to dent‐sterile Ga1‐s popcorn

Herbicide drift exposure leads to reduced herbicide sensitivity in Amaranthus spp.

Protoporphyrinogen oxidase (PPO) inhibitor–resistant waterhemp (Amaranthus tuberculatus) from Nebraska is multiple herbicide resistant: confirmation, mechanism of resistance, and management

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