Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

Liao, Jugou; Dai, Jinran; Yang, Shiyi; Zhou, Xiaoli; Ren, Lihui; Chen, Zhuangzhuang; He, Haili; Chen, Suiyun

doi:10.1111/pbr.12474

Cited by 10 publications

(12 citation statements)

References 24 publications

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“…Delayed cross‐pollination has been reported to be effective in overcoming interspecific incompatibility and is applied in the breeding practices for many species (Yan et al 2009; Sun et al 2011; Liao et al 2017). Delayed selfing during pistil senescence also improve self‐compatibility in self‐incompatible plants, which might provide reproductive assurance and is important for the reproduction of annual plants (Stephenson et al 2000; Travers et al 2004; Seed et al 2006; Suetsugu, 2014; Liao et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…( gla .) S K326) was used as the female parent and cross‐pollinated with the pollen of N. alata , where incompatibility occurred in the basal 1/4 part of the style when pollinated at anthesis (0‐day‐P), which means that pollen tubes stopped growing at the basal 1/4 part of the style 25–30 h after pollination (HAP); however, this incompatibility was overcome by delayed pollination of the 6‐day‐old pistil (6 days after emasculation, 6‐day‐P), whereby bundles of pollen tubes travelled through the ECM of TTS at the base of the style and grew into the ovary at 30 HAP, initiating fertilization (Liao et al, 2017). In this research, the physiological, cellular and morphological regulatory mechanisms of delayed pollination in overcoming cross‐incompatibility between Nta .…”

Section: Introductionmentioning

confidence: 99%

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The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

et al. 2020

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Delayed pollination is widely used to overcome pre‐fertilization incompatibility, but its regulatory mechanisms are unclear. When Nicotiana tabacum was cross‐pollinated with pollen of N. alata, the incompatibility occurring in the basal 1/4 region of the style (pollinated at anthesis: 0‐day‐old pistil) was overcome by delayed pollination (of 6‐day‐old pistil), and the morphological changes and corresponding physiological basis are explored here. The structure and ultrastructure of the pistil were observed under fluorescence microscopy and transmission electron microscopy. Differentially expressed proteins were screened with a monoclonal antibody chip for Nicotiana, and protein expression and distribution were analysed by immunofluorescence. Cellulase and pectinase activities were tested using enzyme‐linked immunosorbent assay kits. The style of Nicotiana is solid in the basal region and pollen tubes grow in the extracellular spaces (ECM) of the transmitting tissue (TTS) cells. Seven of the 22 identified proteins were cell wall‐associated proteins and were expressed at higher levels during pistil senescence. Cellulase and pectinase activities increased. The TTS cells in the basal 1/4 region of the 0‐day‐old style were polygonal and tightly arranged, with narrow ECM, but these were oval or partially dissolved in the 6‐day‐old pistil, leading to wider ECM and richer secretions. The increased expression of cell wall proteins and enhanced enzyme activity during pistil senescence might partially be responsible for the cells becoming oval and the ECM enlarged, providing the morphological basis for delayed pollination overcoming the pre‐fertilization incompatibility between N. tabacum and N. alata.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

et al. 2020

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“…The receiver operating characteristic (ROC) curve, widely used in the prediction of the potential suitable habitats of species 30 , is a highly recognized parameter for the evaluation of diagnostic tests. The area under the ROC curve is AUC (Area Under Curve) which is used to analyze the model accuracy.…”

Section: Model Performance Evaluation and Area Calculationmentioning

confidence: 99%

“…At present, scholars about N. alata at home and abroad had focused on the expression pattern analysis of essential genes 1,28,29 , cultivation management [30][31][32][33] and characteristics of fertilizer requirements, selfincompatibility mechanism and regeneration system 29,34−36 , etc. No research has been done on the distribution and growing areas of N. alata. Here, in order to provide a reference for the planting of N. alata, MaxEnt ecological niche model was used to predict the range of suitable planting areas for N. alata under current and future climate conditions.…”

Section: Introductionmentioning

confidence: 99%

Prediction of global potential suitable habitats of Nicotiana alata Link et Otto based on MaxEnt model

Zhang

Chen

Gao

et al. 2022

Preprint

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Nicotiana alata Link et Otto, widely used in landscaping, is not only of great ornamental value but also of high commercial and medical value. The global potential habitat of N. alata and the environmental factors affecting its distribution are not that clear at present. To provide a reference for the reasonable and extensive planting of N. alata now and in the future, MaxEnt model was used to predict its global suitable habitats under current and future climate conditions, respectively, based on global geographic distribution data of N. alata and the current and future world bioclimatic variables. The results showed that mean annual temperature (bio1), annual precipitation (bio12), temperature seasonality (bio4), and mean temperature of driest quarter (bio9) were the key bioclimatic variables governing the distribution of N. alata. The global suitable habitats of N. alata were mainly distributed in Europe, the United States, Mexico, southeastern South America, and southern Africa under current climate conditions. Compared with current climate conditions, the future climate decreased suitable habitats of N. alata under SSP1-2.6 scenario and increased suitable habitats of N. alata under SSP2-4.5, SSP3-7.0 and SSP5-8.5 climatic scenarios. The results provided valuable information and theoretical reference for the reasonable planting of N. alata.

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“…Otros ejemplos en los cuales se haya empleado el rescate embrionario para la obtención de híbridos interespecíficos con fines ornamentales, se encuentran en los géneros Begonia (Chen, 2012), Dendranthema (Xi Cheng, 2010), Alstroemeria L., (Van Tuyl y De Jeu, 2005). Dentro de la familia Solanaceae estas técnicas han sido utilizadas con éxito en géneros como Capsicum (Debbarama, 2013), Nicotiana (Liao, 2017), Solanum (Gaiero, 2017). El rescate embrionario presenta dos puntos a tener en cuenta, el primero es el aislamiento del embrión, y aquí se debe considerar el estado del mismo, y la metodología utilizada.…”

Section: Introduccionunclassified

PRODUCCIÓN DE HÍBRIDOS INTERESPECÍFICOS ENTRE Nierembergia ericoides Y Nierembergia linariaefolia MEDIANTE LA APLICACIÓN DE TÉCNICAS DE RESCATE EMBRIONARIO

Milicia

Chiesa

Coviella

et al. 2018

Chil. j. agric. anim. sci.

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La hibridación interespecífica es una de las técnicas más utilizadas en el mejoramiento de plantas ornamentales, debido a su alto potencial para obtener vigor híbrido y variación en caracteres, aspectos que no se expresan en las especies originales. El objetivo del presente estudio es obtener híbridos interespecíficos de Nierembergia ericoides miers × N. linariaefolia Graham utilizando el rescate embrionario, a través del cultivo de óvulos como herramienta para sortear las barreras post-cigóticas. Los óvulos fueron aislados 7 días luego del cruzamiento-polinización y cultivados en medio Murashige y Skoog (MS) modificado. Se realizaron tres fechas de siembra: A: 3/10/17; B: 3/11/17; C: 13/12/17. Los óvulos cuyos embriones iniciaron su desarrollo emitiendo la radícula, se repicaron a tubos de ensayo con medio de cultivo MS modificado. Los tubos se colocaron en cámara de cultivo a 25°C con fotoperíodo de 12 horas e intensidad de 4000 lux. Se comparó el número de óvulos germinados respecto al total sembrados y la cantidad de plántulas obtenidas respecto de los óvulos germinados. Se obtuvieron cinco plántulas de 931 óvulos sembrados en la primera fecha, mientras que en la segunda, se obtuvieron cinco de 642 óvulos sembrados. En la tercera fecha, hubo contaminación fúngica, lo que impidió registrar datos. Se puede concluir que la técnica de rescate embrionario a partir del cultivo de óvulos de Nierembergia permitió obtener exitosamente cruzamientos efectivos entre especies incompatibles. Palabras clave: barreras post-cigóticas, plantas ornamentales, mejoramiento, cultivo in vitro, plantas nativas.

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Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

Cited by 10 publications

References 24 publications

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

Prediction of global potential suitable habitats of Nicotiana alata Link et Otto based on MaxEnt model

PRODUCCIÓN DE HÍBRIDOS INTERESPECÍFICOS ENTRE Nierembergia ericoides Y Nierembergia linariaefolia MEDIANTE LA APLICACIÓN DE TÉCNICAS DE RESCATE EMBRIONARIO

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