Interspecific Hybrid Sterility in Rice Is Mediated by OgTPR1 at the S1 Locus Encoding a Peptidase-like Protein

Xie, Yongyao; Xu, Peng; Huang, Jianle; Ma, Shengjian; Xie, Xianrong; Tao, Dayun; Chen, Letian; Liu, Yao-Guang

doi:10.1016/j.molp.2017.05.005

Cited by 76 publications

(70 citation statements)

References 13 publications

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“…S3). These results suggested that the 5-bp deletion in the SSP gene is the cause of the S 1 mut allele in Acc108S 1 M. The SSP gene was located ∼7 kb from the OgTPR1 gene, a gene recently reported to be involved in S 1 locus-mediated siTRD (22). Although both these genes encode a protein containing trypsin-like peptidase domains, there is less than 12.5% identity between the domains encoded by these genes (Fig.…”

Section: Resultsmentioning

confidence: 87%

“…These results suggested that the ability to induce sterility and siTRD is due to the SSP-gla gene and other factor(s): the S 1 locus consists of multiple genes, and the epistatic interaction between them induces hybrid sterility and the S 1 locus-mediated siTRD. Recently, Xie et al (22) suggested the involvement of another gene, OgTPR1, in the S 1 locus-mediated siTRD. However, it was still unclear whether the presence of OgTPR1 is sufficient for the S 1 locus-mediated siTRD.…”

Section: Discussionmentioning

confidence: 99%

“…This work provides mechanistic and evolutionary insights into hybrid sterility and demonstrates the feasibility of the approach that allows broader access to desirable traits in distantly related species during crop breeding. rice (21)(22)(23). However, such reverse-genetic approaches need detailed information about the causative genes before application; therefore, these approaches are not practical for disrupting reproductive barriers for which the causative genes have not been characterized.…”

Section: Significancementioning

confidence: 99%

“…S2). A recent attempt at a CRISPR/Cas9-mediated knockout of OgTPR1, a gene coding a protein containing trypsin-like peptidase and ribosome biogenesis regulatory domains located in the S 1 locus, resulted in an increase in the level of pollen and seed fertility in the heterozygote (S 1 g /S 1 s ) (22). Although this study showed that OgTPR1 is necessary for the S 1 locus-mediated siTRD and suggested that a nucleotide substitution causes a functional change between OgTPR1 and its allelic counterpart OsTP1, whether OgTPR1 is sufficient for inducing this phenomenon has not been examined.…”

Section: Significancementioning

confidence: 99%

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Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice

Koide

Ogino

Yoshikawa

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Understanding the genetic basis of reproductive barriers between species has been a central issue in evolutionary biology. The locus in rice causes hybrid sterility and is a major reproductive barrier between two rice species, and The-derived allele (denoted ) on the locus causes preferential abortion of gametes with its allelic alternative (denoted ) in/ heterozygotes. Here, we used mutagenesis and screening of fertile hybrid plants to isolate a mutant with an allele, , which does not confer sterility in the/ and / hybrids. We found that the causal mutation of the allele was a deletion in the peptidase-coding gene (denoted "") in the locus of No orthologous genes of were found in the genome. Transformation experiments indicated that the introduction of in carriers of the allele did not induce sterility. In / heterozygotes, the insertion of led to sterility, suggesting that complemented the loss of the functional phenotype of the mutant and that multiple factors are involved in the phenomenon. The polymorphisms caused by the lineage-specific acquisition or loss of the gene were implicated in the generation of hybrid sterility. Our results demonstrated that artificial disruption of a single gene for the reproductive barrier creates a "neutral" allele, which facilitates interspecific hybridization for breeding programs.

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Section: Resultsmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

Section: Significancementioning

confidence: 99%

See 2 more Smart Citations

Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice

Koide

Ogino

Yoshikawa

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…SaF/SaM in the sterility locus Sa and OgTPR1 in the locus S1 [61], were disrupted in order to overcome the reproductive barriers in the japonica-indica hybrids. The knockout of one or other Shen et al [62], found The knockout in the indica allele Sc-I of one or two copies of the Sc gene has found that it has, in Japanese-indica hybrids, also rescued the male fertility. Similarly, The increase in fertility of the Japonica-indica hybrids in the knockout of the ORF2 toxin genes, responsible for the newly found selfish-gene suicide mechanism in rice [63].…”

Section: Plant Breeding On Fast Tractmentioning

confidence: 99%

Current and Future Prospects of Plant Breeding with CRISPR/Cas

Wagh

Pohare

2019

CJAST

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Innovative plant breeding technology is an absolute necessity to enhance agriculture production in order to have an ambition of feeding nutritious food to the ever-increasing population. Current advances in CRISPR/Cas genome editing technology have led to effective targeted changes in most plants that promise to accelerate crop improvement. Here we discussed the discovery of CRISPR/Cas technology, associated manipulations for plant genome editing and its potential applications in the plant breeding. We emphasized mainly on the most essential applications of CRISPR/Cas genome editing in crop improvement, such as crop trait improvement (yield and biotic/abiotic stress tolerance), developments in optimizing gene regulation, strategies for generating virus resistance in plants, and the use of high throughput mutant libraries. Finally, the challenges and opportunities for plant breeding in precision agriculture and its bright future discussed.

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CRISPR/Cas9‐Based Genome Editing and its Applications for Functional Genomic Analyses in Plants

2019

Small Methods

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The ability to modify complex genomes precisely to create specific mutants is the holy grail of basic research and applied genetics in the postgenomic era. Programmable sequence‐specific nucleases (e.g., zinc finger nucleases, transcription activator‐like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) systems (e.g., CRISPR/Cas9)), which induce targeted DNA breaks that are then repaired by endogenous repair machinery to generate mutants in a variety of organisms, are being developed at an unprecedented rate. Herein, this paper reviews the history, structure, and biological function of CRISPR/Cas systems, describing how it has been adapted for genome editing (especially CRISPR/Cas9, which has prompted a genome engineering revolution), and emphasizes recent applications and advances in the functional annotation of plant genomes and crop genetic improvement. In addition, the challenges of using the CRISPR/Cas9 system and strategies for improving its specificity are discussed. This review also introduces the creation of CRISPR‐edited DNA‐free plants, which may be more publicly acceptable than other genetically modified organisms. Finally, the future directions and applications of the CRISPR/Cas9 system are speculated on.

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Interspecific Hybrid Sterility in Rice Is Mediated by OgTPR1 at the S1 Locus Encoding a Peptidase-like Protein

Cited by 76 publications

References 13 publications

Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice

Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice

Current and Future Prospects of Plant Breeding with CRISPR/Cas

CRISPR/Cas9‐Based Genome Editing and its Applications for Functional Genomic Analyses in Plants

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