Interspecific hybrids between Lilium nobilissimum and L. regale produced via ovules-with-placental-tissue culture

Obata, Yumi; Niimi, Yoshiji; Okazaki, Keiichi; Miyajima, Ichiro

doi:10.1016/s0304-4238(99)00115-6

Cited by 27 publications

(21 citation statements)

References 21 publications

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“…Ovaries were collected 15 days after cut-style pollination and surface sterilized as described by Obata et al (2000). Ovary-slice culture (Hayashi et al, 1986;Kanoh et al, 1988) was then carried out.…”

Section: Plant Materialsmentioning

confidence: 99%

Interspecific Lily Hybrids with the Ability to Flower Precociously and to Produce Multiple Flower Stalks from Lilium formosanum

Saruwatari

Shuto-Nakano

Nakano

et al. 2008

J. Japan. Soc. Hort. Sci.

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Lilium formosanum Wallace has remarkable traits such as 'precocious flowering' ability, i.e., it reaches anthesis within 12 months from seed germination, with multiple shooting of flower stalks. To verify the possibility of the usefulness of these traits in lily breeding, nine combinations of interspecific crosses (L. formosanum as the seed parent; L. auratum, L. speciosum, L. regale, 'Lollypop', 'Pink Tiger', 'Zaza', 'Le Reve', 'Marco Polo', and 'African Queen' as pollen parents) were carried out with cut-style pollination and ovary-slice culture. Germination was observed in all nine interspecific crosses and 53 hybrids were obtained. Thirty (56.6%) of the 53 hybrids and two self-pollinated progenies of L. formosanum reached anthesis within 24 months from germination through ovaryslice culture. Multiple shooting of flower stalks was recognized in 11 (36.7%) of those flowered hybrids. Four hybrids, the pollen parents of which were Asiatic hybrid lilies with colored flowers, expressed 'precocious flowering' ability, multiple shooting of flower stalks, and entirely colored flowers simultaneously. These results suggest the possibility of breeding new types of cultivars with triple favorable traits from the cross between L. formosanum and Asiatic hybrid lilies with colored flowers.

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Section: Plant Materialsmentioning

confidence: 99%

Interspecific Lily Hybrids with the Ability to Flower Precociously and to Produce Multiple Flower Stalks from Lilium formosanum

Saruwatari

Shuto-Nakano

Nakano

et al. 2008

J. Japan. Soc. Hort. Sci.

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“…RAPD analysis was performed as previously described (Obata et al, 2000) with several modifications. Three sets of DNA Oligomer (12) Set, C-3, G-4, and G-5 (Wako Pure Chemical Industries, Ltd., Osaka, Japan) were tested.…”

Section: Rapd Analysismentioning

confidence: 99%

“…and G. rothschildiana O'Brien , the applicability of this method for detecting hybrids of a wide range of cross combinations in Colchicaceous ornamentals has not yet been examined. On the other hand, among various molecular analyses, RAPD analysis requires only small samples for DNA extraction and only simple PCR techniques, and has been utilized for hybrid identification in various ornamentals (Kato et al, 2001;Nimura et al, 2003;Obata et al, 2000;Takatsu et al, 2001). In the present study, we examined early confirmation of the hybridity by FCM and RAPD analyses of a number of plantlets derived from various intra-and intergeneric crosses among Colchicaceous ornamentals .…”

Section: Introductionmentioning

confidence: 99%

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

淳二

幸子

陽子

et al. 2007

Engei Gakkai Zasshi

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Plantlets derived from ovule culture following intra-or intergeneric crosses among Colchicaceous ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., were subjected to flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses in order to verify their hybridity. For crosses between plants with apparently distinct relative fluorescence intensity (RFI) of nuclei, i.e., intrageneric crosses between Gloriosa genotypes with different ploidy levels, and most intergeneric crosses, detection of hybrid plantlets could be readily accomplished by FCM analysis. The results of hybrid identification by RAPD analysis supported those of FCM analysis. In addition, RAPD analysis allowed the verification of the hybridity of intra-or intergeneric cross-derived plantlets, which could not be identified as hybrids by FCM analysis due to the similarity of RFI of the parents or appearance of the RFI peak in an unexpected position. Totally, 110 independent hybrid plantlets (60 intrageneric and 50 intergeneric hybrids) have so far been identified by FCM and/or RAPD analyses. Thus, FCM in combination with RAPD analyses offer simple and rapid means for the early detection of intrageneric and intergeneric hybrids in Colchicaceous ornamentals.

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“…Interspecific hybridization is often prevented by a cross-incompatibility barrier, but such barriers can partly be overcome by embryo rescue techniques such as embryo, ovule, and immature seed cultures. Interspecific and intergeneric hybrids have successfully been obtained by embryo rescue techniques in a wide range of ornamental plants, Lilium (Obata et al, 2000;Van Tuyl et al, 1991), Zantedeschia (Yao et al, 1994), Primula (Amano et al, 2006;Kato et al, 2008), Colchicaceae plant (Amano et al, , 2009, and Cyclamen (Ishizaka, 2008). In Lychnis, only one viable hybrid plantlet has been obtained successfully between…”

Section: Introductionmentioning

confidence: 99%

Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii

Nakano

Kuwayama

Oka

et al. 2013

J. Japan. Soc. Hort. Sci.

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Lychnis belongs to Caryophyllaceae and contains a number of horticulturally attractive species. In order to widen their variations in horticultural traits, interspecific cross-pollination and subsequent immature seed culture were carried out using 8 Lychnis species, L. chalcedonica, L. coronata, L. fulgens, L. gracillima, L. kiusiana, L. miqueliana, L. sieboldii, and L. wilfordii. Enlarged fruits containing immature seeds were obtained in the 26 cross-combinations 4 weeks after pollination. Immature seeds were isolated from the fruits and cultured on halfstrength Murashige and Skoog medium without plant growth regulators, on which germination occurred in 11 cross-combinations and seedlings were produced in 8 cross-combinations. However, green seedlings were obtained only in L. fulgens × L. sieboldii, and seedlings obtained from the other 7 cross-combinations,kiusiana, and L. wilfordii × L. sieboldii, were albino and died during acclimatization. The hybridity of all 55 green seedlings obtained from L. fulgens × L. sieboldii was confirmed by random amplified polymorphic DNA analysis. Although some morphological variations were observed among hybrids, hybrid plants generally showed intermediate morphologies between the parents. Hybrids showed high pollen fertility and their selfpollination yielded viable seeds. The present study shows the possibility of interspecific cross-breeding in Lychnis. Further studies are necessary to improve the culture conditions of immature seeds and to examine interspecific cross-compatibility using a wide range of Lychnis species.

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Interspecific hybrids between Lilium nobilissimum and L. regale produced via ovules-with-placental-tissue culture

Cited by 27 publications

References 21 publications

Interspecific Lily Hybrids with the Ability to Flower Precociously and to Produce Multiple Flower Stalks from Lilium formosanum

Interspecific Lily Hybrids with the Ability to Flower Precociously and to Produce Multiple Flower Stalks from Lilium formosanum

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, <i>Gloriosa</i> spp., <i>Littonia modesta</i> Hook. and <i>Sandersonia aurantiaca</i> Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii

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