Interspecific Hybrids of Cyclamen Persicum Mill. X Cyclamen Purpurascens Mill.: Propagation, Somaclonal Variation, Resistance to Fusarium Wilt and Suitability as an Outdoor Crop

Ewald, A.; Orlicz-Luthardt, A.; Winkelmann, Traud; Schwenkel, H.-G.

doi:10.17660/actahortic.2000.508.55

Cited by 7 publications

(4 citation statements)

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“…Another proof of the applicability of the regeneration protocol was given for vegetative propagation of sterile interspecific hybrids between Cyclamen persicum and C. purpurascens (Ewald, 1996;Ewald et al, 2000). Out of 2,295 regenerated hybrid plants, 95.4% were true-to-type, confirming the high stability obtained in this regeneration system.…”

Section: Applications Of Somatic Embryogenesis In Propagation and Breeding Of Cyclamenmentioning

confidence: 70%

“…Out of 2,295 regenerated hybrid plants, 95.4% were true-to-type, confirming the high stability obtained in this regeneration system. The types of aberrations, which were observed, comprised changed flower colours and tetraploidy (Ewald et al, 2000). The interspecific hybrids were propagated via somatic embryogenesis in a commercial scale of more than 200,000 plants per year.…”

Section: Applications Of Somatic Embryogenesis In Propagation and Breeding Of Cyclamenmentioning

confidence: 99%

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Towards a Better Understanding of Somatic Embryogenesis in Cyclamen Persicum

Winkelmann¹,

Rode²,

Bartsch³

et al. 2011

Acta Hortic.

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Somatic embryogenesis in Cyclamen persicum was first reported in 1984 and has potential applications for propagation and breeding of this economically important ornamental crop. This in vitro regeneration system can be used for vegetative propagation of parental lines of F 1 hybrids and elite plants, production of artificial seeds, Agrobacterium tumefaciens-mediated genetic transformation, long-term cryopreservation, protoplast to plant regeneration and somatic hybridization. Somatic embryogenesis was shown to be a powerful propagation system for some C. persicum genotypes, but commercial application in large scale so far is hindered by several limitations, i.e., asynchronous development, malformations or secondary somatic embryogenesis. However, recent molecular approaches by transcriptomic and proteomic analyses were undertaken in order to better understand and control this in vitro regeneration system and to overcome these problems. Our studies aim at comparing somatic embryos to their zygotic counterparts regarding their proteomes. Protein separation by two dimensional isoelectric focusing -sodium do-decyl sulfate polyacrylamide gel electrophoresis led to a resolution of about 1000 protein spots per gel, of which the first 253 were identified by mass spectrometry. Most were found to be involved in glycolysis/gluconeogenesis and stress response pathways. A proteome reference map of zygotic embryos will be publicly released soon and may serve as a basis for further investigations and improvements of somatic embryogenesis.

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Section: Applications Of Somatic Embryogenesis In Propagation and Breeding Of Cyclamenmentioning

confidence: 70%

Section: Applications Of Somatic Embryogenesis In Propagation and Breeding Of Cyclamenmentioning

confidence: 99%

Towards a Better Understanding of Somatic Embryogenesis in Cyclamen Persicum

Winkelmann¹,

Rode²,

Bartsch³

et al. 2011

Acta Hortic.

Self Cite

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“…These incompatibility barriers have Electronic supplementary material The online version of this article (doi:10.1007/s11240-010-9674-z) contains supplementary material, which is available to authorized users. been overcome in various combinations of different cultivars of C. persicum with almost ten different wild species of Cyclamen using the embryo rescue technique Uematsu 1992, 1995;Ishizaka 1996Ishizaka , 2003Ishizaka , 2008Ewald 1996;Ewald et al 2000). In the last 150 years, several attempts have been made to hybridise C. persicum (2n = 48 chromosomes) and C. coum (2n = 30 chromosomes) (A.…”

Section: Introductionmentioning

confidence: 99%

Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis

Prange

Serek

Bartsch

et al. 2010

Plant Cell Tiss Organ Cult

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Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 9 10 5 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.

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“…More detailed analyses of the characters of interspecific hybrids were reported for the combinations of C. persicum with C. purpurascens (Ishizaka and Uematsu 1995;Ewald, 1996) with C. hederifolium (Ishizaka and Uematsu 1994) and with C. greacum (Ishizaka 2003). The interspecific hybrids C. persicum · C. purpurascens were successfully propagated in vitro (Ewald et al 2000) and introduced into the market, where they are currently sold as new ornamental plants (Dr. A. Ewald, personal communication).…”

Section: Introductionmentioning

confidence: 99%

Efficient plant regeneration from protoplasts isolated from embryogenic suspension cultures of Cyclamen persicum Mill.

Winkelmann

Specht

Serek

2006

Plant Cell Tiss Organ Cult

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A protocol for plant regeneration from protoplasts has been developed, and then successfully applied to different genotypes of Cyclamen persicum Mill. Protoplasts were isolated from embryogenic suspension cultures by enzymatic digestion in 2% cellulase R10 and 0.5% macerozyme R10. Yields obtained varied between 1 and 5 · 10 5 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. The highest cell division frequencies were found in modified Kao and Michayluk (1975, Planta 126:105-110) medium containing the same types and concentrations of plant growth regulators that were applied for suspension culture (2.0 mg l -1 2,4-dichlorophenoxyacetic acid and 0.8 mg l -1 6-(c,c-dimethylallylamino)purine). Cell division was recorded for all 11 tested genotypes in frequencies of up to 12% and 18% after 7 and 14 days, respectively. However, cell division frequency varied strongly between different genotypes. After 4-6 weeks calluses were released from the alginate films and further cultured on hormone-containing medium for continued growth or transferred to hormone-free medium for regeneration of somatic embryos. Plant regeneration via somatic embryogenesis succeeded in 9 out of the 11 genotypes under investigation. Up to now protoplast-derived plants from four genotypes have been successfully transferred to soil.

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Interspecific Hybrids of Cyclamen Persicum Mill. X Cyclamen Purpurascens Mill.: Propagation, Somaclonal Variation, Resistance to Fusarium Wilt and Suitability as an Outdoor Crop

Cited by 7 publications

References 0 publications

Towards a Better Understanding of Somatic Embryogenesis in Cyclamen Persicum

Towards a Better Understanding of Somatic Embryogenesis in Cyclamen Persicum

Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis

Efficient plant regeneration from protoplasts isolated from embryogenic suspension cultures of Cyclamen persicum Mill.

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