Interspecific protoplast fusion of atmospheric and room-temperature plasma mutants of <i>Aspergillus</i> generates an L-asparaginase hyper-producing hybrid with techno-economic benefits

Asitok, Atim; Ekpenyong, Maurice; Akwagiobe, Ernest; Asuquo, Marcus Inyama; Rao, Anil V.; Ubi, David; Iheanacho, Juliet; Ikharia, Eloghosa; Antai, Agnes; Essien, Joseph P.; Antai, S. P.

doi:10.1080/10826068.2022.2150643

Cited by 4 publications

(2 citation statements)

References 52 publications

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“…The contents of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharides and total triterpenes of the fusants obtained using protoplast fusion of A. cinnamomea and C. militaris were significantly increased [34]. These results showed that the use of protoplast fusion technology to recombine the nucleus and cytoplasm of parents can significantly increase the content of active substances [46,47].…”

Section: Discussionmentioning

confidence: 79%

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Li,

Liu,

et al. 2023

Horticulturae

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Ganoderma lingzhi is an important medicinal fungus, and it is particularly important to select strains with high yields and active substance contents. In this study, protoplasts of G. lingzhi were thermally inactivated to destroy intracellular enzyme proteins and preserve DNA. The DNA of G. resinaceum was damaged by ultraviolet (UV) radiation, and other components of the protoplasm except DNA were preserved. Then, the protoplast was induced using polyethylene glycol (PEG) for fusion. The results showed that the optimal thermal inactivation conditions for G. lingzhi were 30 min in a 45 °C water bath, and the optimal UV inactivation conditions for G. resinaceum were 70 s of irradiation using a 20 W UV lamp at a vertical distance of 15 cm. Antagonistic tests, internal transcribed space (ITS) and mitochondrial DNA identification, intersimple sequence repeat (ISSR) molecular markers and morphology were used to distinguish the parents from the fusants. Four true fusants were obtained, and the yield was 2.5%. The fruiting body yield of the fusants was significantly higher than that of G. lingzhi, and the polysaccharide and triterpene contents of the RAD-64 fusant were significantly higher than those of G. lingzhi. The results presented in this paper show that protoplast fusion technology can effectively improve G. lingzhi varieties and support the breeding of new varieties.

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Section: Discussionmentioning

confidence: 79%

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Li,

Liu,

et al. 2023

Horticulturae

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“…High-yield mutants U1, A6, and UA13 exhibited impressive productivity enhancements [ 88 ]. Furthermore, ARTP has been successfully utilized for the production of diverse bioenzymes, including tannase [ 89 , 90 ], raw starch-degrading enzymes [ 91 ], thermostable xylanase [ 92 ], cellulose [ 93 , 94 ] and L-asparaginase [ 95 ].…”

Section: Application Of Artp To Fungimentioning

confidence: 99%

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Zhang

Miao

Feng

et al. 2023

CIMB

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Atmospheric and room-temperature plasma (ARTP) is an efficient microbial mutagenesis method with broad application prospects. Compared to traditional methods, ARTP technology can more effectively induce DNA damage and generate stable mutant strains. It is characterized by its simplicity, cost-effectiveness, and avoidance of hazardous chemicals, presenting a vast potential for application. The ARTP technology is widely used in bacterial, fungal, and microalgal mutagenesis for increasing productivity and improving characteristics. In conclusion, ARTP technology holds significant promise in the field of microbial breeding. Through ARTP technology, we can create mutant strains with specific genetic traits and improved performance, thereby increasing yield, improving quality, and meeting market demands. The field of microbial breeding will witness further innovation and progress with continuous refinement and optimization of ARTP technology.

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Application and research progress of ARTP mutagenesis in actinomycetes breeding

Zhu,

Ding,

Rang

et al. 2024

Gene

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Interspecific protoplast fusion of atmospheric and room-temperature plasma mutants of Aspergillus generates an L-asparaginase hyper-producing hybrid with techno-economic benefits

Cited by 4 publications

References 52 publications

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Interspecific Hybridization between Ganoderma lingzhi and G. resinaceum by PEG-Induced Double-Inactivated Protoplast Fusion

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Application and research progress of ARTP mutagenesis in actinomycetes breeding

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