Interspecific recombination among aminoglycoside producing streptomycetes.

Mazieres, N.; Peyre, Marianne; Penasse, L.

doi:10.7164/antibiotics.34.544

Cited by 17 publications

(6 citation statements)

References 9 publications

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“…These include mutasynthesis (15), in which an unnatural precursor is fed to an idiotroph genetically blocked in antibiotic biosynthesis, and hybrid biosynthesis (10,11,13), in which a wild-type strain is fed with such a precursor in the presence of an enzyme inhibitor which phenotypically blocks the biosynthetic pathway of the recipient strain. Another approach to the production of novel antibiotics is interspecific mating (9,14) or protoplast fusion (4,5). In the published examples of this approach, the origin of the novel compounds detected is ambiguous; however, it is likely that an alteration of a hitherto unexpressed capacity for antibiotic production of one of the strains in the partnership by transferring into it regulatory genetic material from the other is the predominant mechanism.…”

mentioning

confidence: 99%

Production of new hybrid antibiotics, mederrhodins A and B, by a genetically engineered strain

Ōmura¹,

Ikeda²,

Malpartida³

et al. 1986

Antimicrob Agents Chemother

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Hybrid antibiotics mederrhodins A and B were produced by a recombinant strain consisting of the medermycin-producing Streptomyces sp. strain AM7161 containing part of the gene clusters for actinorhodin biosynthesis of Streptomyces coelicolor A3(2). Mederrhodin A has a hydroxyl group at the C-6 position of the medermycin molecule, and mederrhodin B is dihydromederrhodin A. The antimicrobial activity of mederrhodin A resembled that of medermycin. Mederrhodin B was almost devoid of antimicrobial activity.

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mentioning

confidence: 99%

Production of new hybrid antibiotics, mederrhodins A and B, by a genetically engineered strain

Ōmura¹,

Ikeda²,

Malpartida³

et al. 1986

Antimicrob Agents Chemother

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“…However, the production of hybrid antibiotic molecules by the interspecific crosses has been observed only by MAZIEREs et al 3) and SCHLEGEL & FLECK2). We attempted interspecific protoplast fusion between macrolide-producing streptomycetes to produce hybrid macrolide antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Interspecific protoplast fusion among macrolide-producing streptomycetes.

et al. 1984

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From auxotrophic and idiotrophic mutants of Streptomyces fradiae (tylosin producer) and Streptomyces sp. AM 4900 (pikromycin producer) or Streptomyces narbonensis (narbomycin producer), prototrophic fusants were obtained at a low frequency by the protoplast fusion technique.In the cross of S. fradiae 261-27E (mvcaminose-idiotroph, ilr) and Streptomyces sp. AM 4900 N3-4, (pikronolide-idiotroph, arg), an unstable prototrophic fusant. strain No. 14, produced a macrolide antibiotic which was not produced by the wild type, parent strains, and the productivity was lost within a few times transfer. It was concluded that the fusant ssas not a recombinant, but a heterokaryon.On the other hand, relatively stable fusants were obtained from the cross of S. fradiae TBM (mycaminose-idiotroph, met) and S. narbonensis NA12US3 (narbonolide-idiotroph, his, str) at a frequency of 3.2' ' 10-5 One of the prototrophic fusants produced narbomvcin, which is believed to be due to a result of interspecific recombination.

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“…First, with the introduction of foreign genes or alleles into populations, new characters can emerge (Alacevic, 1963;Mazieres et al, 1981), benefiting fast spreading of resistance or adaptive capabilities, which are sometimes conferred by interspecies recombination of housekeeping genes (Freel et al, 2013;Munoz et al, 1995). Next, population genetic diversity can be enriched and hybrid populations may subsequently appear once various foreign genes become highly adaptive, sweep and reach fixation (Andam et al, 2016;Doroghazi and Buckley, 2010).…”

Section: Interspecies Recombination In Streptomycetesmentioning

confidence: 98%

“…This system is distinctly different from that employed by most other unicellular Gram-positive bacteria, which requires multiple proteins to transfer single-stranded DNA and is termed the type IV secretion system (Goessweiner-Mohr et al, 2013). Research on Streptomyces interspecies conjugation has long been reported (Alacevic, 1963;Mazieres et al, 1981) and confirmed throughout the literature (Pettis and Cohen, 1994;Possoz et al, 2001;Vogelmann et al, 2011). More recently, studies on population genetics have revealed a cohesive force of homologous recombination in Streptomyces populations (Cheng et al, 2015;Doroghazi and Buckley, 2010) and displayed the frequent transfer of housekeeping gene fragments in Streptomyces (Andam et al, 2016;Doroghazi and Buckley, 2010), catching a glimpse of the promiscuous genetic exchange among Streptomyces species.…”

Section: Introductionmentioning

confidence: 98%