Interspecific somatic hybridization in potato by protoplast electrofusion

Serraf, I.; Sihachakr, D.; Ducreux, G.; Brown, Spencer; Michè,; Allot, e; Barghi, Nasrine; Rossignol, L.

doi:10.1016/0168-9452(91)90225-w

Cited by 47 publications

(26 citation statements)

References 40 publications

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“…torvum (Jadari et al ., 1992) . Furthermore, somatic hybrids between the sexually-compatible species S. berthaultii and potato have been produced (Serraf et al ., 1991) . The most widely-used wild species in somatic hybridization experiments is the diploid non-tuberous wild species S .…”

Section: Potatomentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization . In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed . It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop . Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes . Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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Section: Potatomentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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“…The first successful protoplast electrofusion was performed between S. tuberosum and S. phureja (Puite et al, 1986). Other sexual compatible or incompatible Solanum wild species used for somatic hybridizations have been S. berthaultii (Serraf et al, 1991), S. torvum (Jadari et al, 1992), S. circaeifolium , S. bulbocastanum (Austin et al, 1993), S. commersonii (Cardi et al, 1993), S. etuberosum (Novy & Helgeson, 1994), S. papita (Kaendler et al, 1996) and S. pinnatisectum (Menke et al, 1996). Phenotypic characterisation revealed a general vegetative hybrid vigour in most combinations.…”

Section: Introductionmentioning

confidence: 97%

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

et al. 2000

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SummarySomatic fusions between an accession of the diploid wild species Solanum verrucosum and a dihaploid S. tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were Qbtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

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“…Somatic hybridization provides a means of bypassing sexual incompatibilities and offers the possibility of increasing genetic variability for nuclear and cytoplasmic genomes (Belliard et al 1979; reviewed by Medgyesy 1990), and also of transferring resistance. In the past decade, the potential use of somatic hybridization has been demonstrated by the successful incorporation of resistance to diseases (Austin et al 1985; Gibson et al 1988;Jadari et al 1992) and insects (Serraf et al 1991) from wild Solanum species into potato.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii

Sherraf

Tizroutine

Chaput

et al. 1994

Plant Cell Tiss Organ Cult

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Mesophyll protoplasts of Lycopersicon pennelli Corr., a wild relative of tomato, were electrofused with those from a dihaploid potato clone, cv Nicola, with the objectives of transferring saline tolerance from L. penneUii to cultivated potato. 150 calli were selected from the fusion experiments, finally giving 2 hybrid shoots. Their hybrid nature was verified by examining isoenzyme patterns for esterases (EST), peroxidase (PRX), phosphogluconate dehydrogenase (6-PGD), and glutamate oxaloacetate transaminase (GOT). The hybrid plants had an intermediate morphology, and grew vigorously in vitro. When transplanted to soil, they were less vigorous, due to difficulties in rooting, but were still capable of flowering, and forming short stolons and mishaped tubers, probably resulting from the effects of gene dosage due to the novel association of two genomes from a tuberizing (potato) and a non tuberizing species (L. pennellii). The characteristics of such mishaped tubers provided strong evidence of a hybrid nature for the selected plants. The hybrid plants were highly sterile, producing only 3-7% viable pollen. Tests for salt tolerance showed that the growth of the somatic hybrid plants was reduced by 50% as for L. penneUii, whilst potato did not grow at all under saline conditions.

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Interspecific somatic hybridization in potato by protoplast electrofusion

Cited by 47 publications

References 40 publications

The potential of somatic hybridization in crop breeding

The potential of somatic hybridization in crop breeding

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii

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