Interspecific spread of dsRNA mycoviruses in entomogenous fungi i spp.

Ning, Siyu; Kang, Qin; Liu, Hongyu; Lu, Yang; Sui, Li; Xu, Wei; Shi, Wangpeng; Li, Qiyun; Zhang, Zhengkun

doi:10.1016/j.virusres.2022.198933

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…Liu et al found that SsHADV-1 could not only infect its host fungus extracellularly, but also the mycophagous insect Lycoriella ingenua, which was used as a transmission vector for virus spread, and more importantly, this virus could multiply and transmit vertically within the life cycle of insects [67]. Our previous research found that BbCV2 could spread from B. bassiana to another two Beauveria species via not only hyphal anastomosis but also insect coinfection [29]. In the present study, we observed e cient transmission from virus-infected strains to virus-free strains of B. bassiana, as well as vertical transmission in the same strain.…”

Section: Discussionmentioning

confidence: 99%

“…Isogenic strains with and without BbCV2 infection were obtained via the insect coinfection method as described previously [29]. Coinfected insect bodies harbouring the virus-infected donor strain BbOFZK152 and virus-free recipient strain BbOFDH1-5-GFP were fed at 25 ℃ for 7 to 10 days as described previously [31]to obtain muscardine cadavers.…”

Section: Fungal Materials and Horizontal Transmission Of Virusesmentioning

confidence: 99%

“…Three virus-infected BbOFDH1-5-GFP strains, designated BbOFDHCV1, BbOFDHCV2 and BbOFDHCV3, as well as three virus-free strains BbOFDH1, BbOFDH2 and BbOFDH3 derived from monospores of BbOFDH1-5-GFP, were randomly selected for virulence evaluation, virus detection and transcriptomic analysis. Evaluation of vertical spread e ciency of BbCV2 was performed as described previously [29], in which the three virus-infected strains above were subcultured on PDA medium for three generations and veri ed by dsRNA extraction and RT-PCR. Tests for each strain were repeated three times.…”

Section: Fungal Materials and Horizontal Transmission Of Virusesmentioning

confidence: 99%

“…To explore these questions, we selected the mycovirus Beauveria bassiana chrysovirus 2 (BbCV2) that have identi ed in previous work [30], which infects various Beauveria species [29], and investigated whether the virus (1) can decrease the pathogenicity of the host B. bassiana strain; (2) is prevalent and genetically stable in wild B. bassiana populations in the eld; and (3) can dissociate outside host fungus cells to infect other strains; we also (4) probed the mechanism through which mycoviruses affect host fungi. The results help to reveal whether mycoviruses are key factors causing B. bassiana hypovirulence in preservation and eld applications.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is unclear whether mycoviruses have the ability to cause epidemics, and thereby induce hypovirulence of B. bassiana populations in the eld, even though mycoviruses are able to undergo interspeci c, intraspeci c and vertical transmission in Beauveria spp. [29]. Therefore, whether mycoviruses are transmitted through direct hyphal contact or migrate outside to infect other host cells following their replication in host fungal cells remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Hypovirulence-associated Mycovirus Epidemics Cause Pathogenicity Degeneration of Beauveria bassiana in the Field

Zhang,

Guo,

et al. 2023

Preprint

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Background The entomogenous fungus Beauveria bassiana is used as a biological insecticide worldwide, but its application is affected by pathogenicity degeneration in the field. Previous studies showed that multiple factors contribute to this phenomenon. Mycovirus infection causes hypovirulence of phytopathogenic fungi and mycoviruses have been used for plant disease biocontrol. However, it remains unknown whether mycovirus epidemics are key factors causing hypovirulence of B. bassiana naturally in the field. Methods A mycovirus Beauveria bassiana chrysovirus 2 (BbCV2) we have previously identified was employed to clarify its impact on the pathogenicity of host fungi Beauveria bassiana against the larvae of insect pest Ostrinia furnacalis. Wild strains of B. bassiana were collected from different geographic locations in Jilin Province, China, to clarify the epidemic and genetic diversity of the mycovirus BbCV2. The serological analysis was conducted by preparing polyclonal antibody against a BbCV2 coat protein, to determine whether the it can dissociate outside the host fungi cells and subsequently infect new hosts. Transcriptome analysis was used to reveal the interactions between viruses and hosts. Results We found that themycovirus BbCV2 possessed efficient and stable horizontal and vertical transmission capabilities and caused B. bassiana hypovirulence. Surprisingly, this virus was prevalent in the field as a core virus in wild B. bassiana strains, without obvious genetic differentiation. The serological results showed that the virus could not only replicate within the host cell, but also dissociate outside to infect new hosts. Transcriptome analysis revealed decreased expression of genes related to insect epidermis penetration and toxin metabolism in B. bassianacaused by mycovirus infection. Conclusion B.bassiana infected by hypovirulence-associated mycovirus can spread the virus to new host strainsafter infecting insects, and causethe virus epidemics in the field. The findings confirmed that mycovirus infection may be an important factor affecting the pathogenicity degradation of B. bassiana in the field.

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Section: Discussionmentioning

confidence: 99%

Section: Fungal Materials and Horizontal Transmission Of Virusesmentioning

confidence: 99%

Section: Fungal Materials and Horizontal Transmission Of Virusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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