Interspecific Transformation in Azotobacter

Sen, Mausumi; Sen, S. P.

doi:10.1099/00221287-41-1-1

Cited by 31 publications

(19 citation statements)

References 5 publications

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“…All the cultures were purified twice before use. (Sen & Sen, 1965 Transformation. Competent bacteria were prepared according to the method of Stuy (1962) with slight modifications.…”

Section: Organismsmentioning

confidence: 99%

Genetic Transformation in Pseudomonas

Khan

Sen

1967

Journal of General Microbiology

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S U h l M A R Ylntraspecific and interspecific transformation in Pseudomonas has been investigated. Two Isoleucine + valiiie requiring mutants of Pseidotnonus neruginosa have been transformed t o prototrophy. By using gelatin liquifaction as a marker character it was observcd that two strains of P.J/iiorescens. a phytopathogen P. niangjferaeirzdic,ric. ii nd ii strain of Pseudon?onas originally isolated from fish were transformable. When P. solanacearum w a s used as a donor, the transformation frequency of P. niangijeraeindicae was vcry high. DNA from fish Pseudomonas 1 z+ax capable of transforming two strains of P. J-luorescens and P. iiiaizSiJerireiiiLli("(iC in addition to fish Pseudoinonas 2 I . When P. jluorescens was treated with the D N A from P. aei.zigiuzo.w transformation frequency was lower ; the cii p c i ty of pigment production, however, could not be transferred to any of tlw strains. The highest transformation frequency was obtained towards the end of the log phase of growth of the receptor bacteria, with a DNA concentration of about 10 ,ug./nil. Optimum temperature for transformation \+a\ 25-30 ' and agitation and star\ ;I tion in diluted media increased the number ol' transformants. The significmce of the observations has been discussed from the point of view of relationships among the strains and species studied.

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“…All the cultures were purified twice before use. (Sen & Sen, 1965 Transformation. Competent bacteria were prepared according to the method of Stuy (1962) with slight modifications.…”

Section: Organismsmentioning

confidence: 99%

Genetic Transformation in Pseudomonas

Khan

Sen

1967

Journal of General Microbiology

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“…It was shown that melanin is an excellent photoprotective agent. Many reports have described melanin-producing microorganisms such as Mycobacterium (Prabhakaran et al, 1968), Bacillus (Aronson and Vickers, 1965), Vibrio (Ivins and Holmes, 1980), Rhizobium (Beynon et al, 1980), Pseudomonas (Delafield et al, 1965), Micrococcus (Mung and Kondrateva, 1981), Gluconobacter (Pavelenko et al, 1981), Azotobacter (Sen and Sen, 1965) and Azospirillum (Lakshmi and Neyra, 1987).…”

Section: Introductionmentioning

confidence: 99%

Characterization of melanin produced by a wild-type strain of Bacillus thuringiensis

Chen

Yinyue

Wang

et al. 2004

J. Gen. Appl. Microbiol.

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Bt L-7601 is a UV resistant wild-type strain, which belongs to Bacillus thuringiensis subsp. dendrolimus serotype H4a4b. It was isolated from nature, and produced a dark brown pigment during the exponential phase of growth. Bt L-7601 had the ability to produce pigment in a general nutrition-abundant medium, which had no L-tyrosine. The pigment was identified as melanin based on chemical testing, its light absorbance, and FT-IR analysis. Bt L-7601 has a strong resistance to UV light. After 30 min irradiation its survival rate was 17 times higher than that of the strain B. thuringiensis subsp. colmeri 15A3, which had no pigment. Results of the bioassays of residual insecticidal activity of Bt formulation with and without pigment produced by Bt L-7601 against larvae of Helicoverpa armigera and Spodoptera exigua after exposure to UV irradiation showed that the pigment is an excellent UV protective agent for the insecticidal proteins.

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“…The eumelanins and pheomelanins are found mainly in animal species, whereas the allomelanins are found in plants and micro-organisms (Nicolaus et al, 1964). Bacteria belonging to the genera Mycobacterium (Prabhakaran et al, 1968), Bacillus (Aronson & Wermus, 1965), Vibrio (Ivins & Holmes, 1980), Rhizobium (Beynon et al, 1980), Pseudomonas (Delafield et al, 1965;Ogunnariuo & Hamilton-Miller, 1979, Micrococcus (Mung & Kondrat'eva, 198 l), Gluconobacter (Pavelenko et al, 1981), Azotobacter (Sen & Sen, 1965) and *Author for correspondence. Tel.…”

Section: Introductionmentioning

confidence: 99%