Interstitial leukocyte migration in vivo

Lam, Pui-ying; Huttenlocher, Anna

doi:10.1016/j.ceb.2013.05.007

Cited by 32 publications

(20 citation statements)

References 72 publications

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“…In neutrophils migrating in vivo, actin stability is polarized, with dynamic F-actin concentrated in leading edge pseudopods and stable F-actin concentrated at the rear, where there is high actomyosin contractility (Yoo et al, 2010). During chemotactic migration, Rac activation at the leading edge induces actin polymerization and pseudopod formation (Charest and Firtel, 2007, Lam and Huttenlocher, 2013). This actin polymerization drives protrusions at the leading edge of neutrophils and contributes to forward migration even when the uropod is compromised (Houk et al, 2012, Lammermann et al, 2008).…”

Section: Front and Rear Signaling And The Maintenance Of Polaritymentioning

confidence: 99%

Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration

2016

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Cell motility is required for diverse biological processes including development, homing of immune cells, wound healing, and cancer cell invasion. Motile neutrophils exhibit a polarized morphology characterized by the formation of leading edge pseudopods and a highly contractile cell rear known as the uropod. Although it is known that perturbing uropod formation impairs neutrophil migration, the role of the uropod in cell polarization and motility remains incompletely understood. Here we discuss cell intrinsic mechanisms that regulate neutrophil polarization and motility with a focus on the uropod, and examine how relationships among regulatory mechanisms change when cells change their direction of migration.

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Section: Front and Rear Signaling And The Maintenance Of Polaritymentioning

confidence: 99%

Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration

2016

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“…In parallel to this work, data on the requirement of microtubules for amoeboid migration in 3D (Box 1) has also been collected (Lam and Huttenlocher, 2013). A collagen-based 3D culture of leukocytes was developed in the 1980s (Grinnell, 1982).…”

Section: Microtubule Requirement In Cell Morphogenesis and Motility Imentioning

confidence: 99%

“…However, soft collagen-rich matrices are considered to mimic well multiple types of in vivo tissue environments (Doyle et al, 2009;. Early research using soft collagen gels and ex vivo cultures led to the definition of two main 3D motility modes: (i) amoeboid cell migration, as seen in leukocytes (Lam and Huttenlocher, 2013;Lammermann and Germain, 2014) and (ii) mesenchymal cell migration, found in fibroblasts, invasive cancer cells and many developmental precursors, especially during EMT (Friedl and Wolf, 2010;Lim and Thiery, 2012). Amoeboid migration is characteristic for rounded cells with relatively low ECM adhesion and relatively high Rho-driven contractility, whereas mesenchymal migration depends on cell elongation associated with relatively high ECM adhesion and Rac1-driven protrusion (Friedl and Wolf, 2010).…”

Section: Box 1 Cell Morphogenesis and Migration In Soft 3d Matricesmentioning

confidence: 99%

Microtubules in 3D cell motility

Bouchet

Akhmanova

2017

Journal of Cell Science

104

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Three-dimensional (3D) cell motility underlies essential processes, such as embryonic development, tissue repair and immune surveillance, and is involved in cancer progression. Although the cytoskeleton is a well-studied regulator of cell migration, most of what we know about its functions originates from studies conducted in twodimensional (2D) cultures. This research established that the microtubule network mediates polarized trafficking and signaling that are crucial for cell shape and movement in 2D. In parallel, developments in light microscopy and 3D cell culture systems progressively allowed to investigate cytoskeletal functions in more physiologically relevant settings. Interestingly, several studies have demonstrated that microtubule involvement in cell morphogenesis and motility can differ in 2D and 3D environments. In this Commentary, we discuss these differences and their relevance for the understanding the role of microtubules in cell migration in vivo. We also provide an overview of microtubule functions that were shown to control cell shape and motility in 3D matrices and discuss how they can be investigated further by using physiologically relevant models.

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“…The ability to move through a three-dimensional (3D) matrix is a physiological feature found in many differentiated cell types and in developmental precursors (Friedl and Gilmour, 2009; Lam and Huttenlocher, 2013; Nakaya and Sheng, 2008). Besides its role in tissue morphogenesis and immune surveillance, cell invasion is also associated with metastasis in solid cancers (Chaffer and Weinberg, 2011).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

et al. 2016

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Summary Microtubules regulate signaling, trafficking and cell mechanics but the respective contribution of these functions to cell morphogenesis and migration in 3D matrices is unclear. Here, we report that the microtubule plus-end tracking protein (+TIP) SLAIN2, which suppresses catastrophes, is not required for 2D cell migration but is essential for mesenchymal cell invasion in 3D culture and in a mouse cancer model. We show that SLAIN2 inactivation does not affect Rho GTPase activity, trafficking and focal adhesion formation. However, SLAIN2-dependent catastrophe inhibition determines microtubule resistance to compression and pseudopod elongation. Another +TIP, CLASP1, is also needed to form invasive pseudopods because it prevents catastrophes specifically at their tips. When microtubule growth persistence is reduced, inhibition of depolymerization is sufficient for pseudopod maintenance but not remodeling. We propose that catastrophe inhibition by SLAIN2 and CLASP1 supports mesenchymal cell shape in soft 3D matrices by enabling MTs to perform a load-bearing function.

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Interstitial leukocyte migration in vivo

Cited by 32 publications

References 72 publications

Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration

Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration

Microtubules in 3D cell motility

Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

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