Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.)

Abstract

Quantitative trait loci (QTL) were mapped in segregating progeny from a cross between two soybean (Glycine max (L.) Merr.) cultivars: 'Minsoy' (PI 27.890) and 'Noir 1' (PI 290.136). The 15 traits analyzed included reproductive, morphological, and seed traits, seed yield and carbon isotope discrimination ratios ((13)C/(12)C). Genetic variation was detected for all of the traits, and transgressive segregation was a common phenomenon. One hundred and thirty-two linked genetic markers and 24 additional unlinked markers were used to locate QTL by interval mapping and one-way analysis of variance, respectively. Quantitative trait loci controlling 11 of the 15 traits studied were localized to intervals in 6 linkage groups. Quantitative trait loci for developmental and morphological traits (R1, R5, R8, plant height, canopy height, leaf area, etc.) tended to be clustered in three intervals, two of which were also associated with seed yield. Quantitative trait loci for seed oil were separated from all the other QTL. Major QTL for maturity and plant height were linked to RFLP markers R79 (31% variation) and G173 (53% variation). Quantitative trait loci associated with unlinked markers included possible loci for seed protein and weight. Linkage between QTL is discussed in relation to the heritability and genetic correlation of the traits.

Use of the QTL approach to the study of soybean trait relationships in two populations of recombinant inbred lines at the F7 and F8 generations

“…Therefore, it can be concluded that the evaluated traits are under environment effects, as well as that the present population can be used in studies aimed at evaluating heritability of such traits. Mansur et al (1993) that the LGs C1, C2 and D1 have loci associated with the traits leaf area and maturity of the soybean. The identification of a QTL associated with W ST in the LG L confirms the observation that in this LG there is a QTL associated with WST (Santos et al, 2006) and plant height of G. max (Lee et al, 1996).…”

Genetic Redundancy in Soybean Photoresponses Associated With Duplication of the Phytochrome A Gene

“…None of the classical major genes controlling flowering and maturing times that have been mapped so far is located in LG O. Many QTL influencing the flowering and maturing times have been allocated to various LGs (Keim et al 1990;Mansur et al 1993;Lee et al 1996;Orf et al 1999;Tasma et al 2001;Yamanaka et al 2001;Chapman et al 2003;Watanabe et al 2004;Zhang et al 2004;Funatsuki et al 2005;Liu et al 2007). However, only a maturitycontrolling QTL has been located in LG O (Zhang et al 2004).…”

Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean

“…Diers et al (1992) used the same interspecific soybean population to identify several molecular marker loci that were significantly associated with QTLs for seed protein and oil. Other workers utilized molecular markers to identify several genomic regions significantly associated with seed protein, oil, seed weight and sucrose content in different intraspecific soybean populations (Mansure et al, 1993;Lee et al, 1996;Mian et al, 1996;Maughan et al, 1996;Brummer et al, 1997). The objective of this study was to use SSR markers to identify quantitative trait loci associated with seed coat permeability and electrolyte leaching in an intraspecific F 2 soybean population.…”