Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model

Abstract

Background. Psoriasis is a common chronic inflammatory skin disease with multifactor etiology, characterized by abnormal proliferation and differentiation of keratinocytes. Huang-Lian Jie-Du decoction (HLJDD) is a traditional Chinese medicine prescription with good clinical curative effect on psoriasis. However, its therapeutic mechanisms are still unclear. Methods. The psoriasis model of SKH-1 nude mice was established by imiquimod-induced and HLJDD gavage was given. Hematoxylin and eosin staining were used to evaluate pathological morphologies, and immunohistochemistry was used to detect the expressions of Wnt1, β-catenin, and c-Myc in psoriasis mice. Western blot was used to examine the expressions of Frizzled-2, LRP5/6, GSK-3β, APC, Axin2, TCF4, LEF1, cyclin D1, TBX3, EPHB2, and NOTUM enzyme. Results. In this study, HLJDD reduced skin erythema and lesions, decreased the thickness of epidermal and downregulated the expressions of Wnt1, β-catenin, and c-Myc. Western blot results showed that HLJDD reduced the expressions of Wnt receptors Frizzled-2 and LRP5/6, and Wnt downstream target genes TCF4, LEF1, cyclin D1, TBX3, and EPHB2, while upregulated destruction complex proteins GSK-3β, APC, and Axin2. Conclusions. HLJDD can effectively treat psoriasis and inhibit the Wnt/β-catenin signaling pathway at multiple stages.

The therapeutic effect of traditional Chinese medicine on breast cancer through modulation of the Wnt/β-catenin signaling pathway

“…For example, HuangLianJieDu Decoction has been observed to attenuate the expression of LRP5/6, Wnt1, and β-catenin, thereby intervening at different junctures of the Wnt/β-catenin signaling pathway. This modulation has proven beneficial in treating conditions like psoriasis ( Yang et al, 2022 ). Similarly, the HuangGan recipe acts by dampening the expression of Wnt1, β-catenin, and transcription factor 4, offering therapeutic advantages in conditions like glomerulosclerosis and tubulointerstitial fibrosis ( Mo et al, 2015 ).…”

“…While these pathways have varying dependencies on β-catenin, together they form an intricate regulatory network. The Wnt/β-catenin signaling pathway, omnipresent in cells, is pivotal in modulating inflammatory reactions (Yang et al, 2022), lessening fibrosis (Mo et al, 2015), and counteracting osteoporosis (Zhang et al, 2017). Its significance is further underscored by its role in the etiology of various diseases.…”