Intervention of cardiomyocyte death based on real-time monitoring of cell adhesion through impedance sensing

Qiu, Yuchen; Liao, Ronglih; Zhang, Xin

doi:10.1016/j.bios.2009.06.027

Cited by 10 publications

(3 citation statements)

References 31 publications

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“…C m was found to be of the order of 1.05 mF cm 22 for pure CM, a value also found previously elsewhere [41]. An a of 2.5 V 1/2 Â cm was comparably described in the literature for pure CM and was related to absolute cell -substrate distances of 59 nm [38,50].…”

Section: Discussionsupporting

confidence: 85%

“…C m was found to be of the order of 1.05 µF cm −2 for pure CM, a value also found previously elsewhere [ 41 ]. An α of 2.5 Ω 1/2 × cm was comparably described in the literature for pure CM and was related to absolute cell–substrate distances of 59 nm [ 38 , 50 ]. Concerning R b , we found an increase by a factor of 2.5–3 (12.6 Ω × cm 2 ) as compared with the monoculture of Fb (5.7 Ω × cm 2 ).…”

Section: Discussionmentioning

confidence: 89%

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Crosstalk of cardiomyocytes and fibroblasts in co-cultures

Rother

Richter

Turco³

et al. 2015

Open Biol.

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Electromechanical function of cardiac muscle depends critically on the crosstalk of myocytes with non-myocytes. Upon cardiac fibrosis, fibroblasts translocate into infarcted necrotic tissue and alter their communication capabilities. In the present in vitro study, we determined a multiple parameter space relevant for fibrotic cardiac tissue development comprising the following essential processes: (i) adhesion to substrates with varying elasticity, (ii) dynamics of contractile function, and (iii) electromechanical connectivity. By combining electric cell-substrate impedance sensing (ECIS) with conventional optical microscopy, we could measure the impact of fibroblast–cardiomyocyte ratio on the aforementioned parameters in a non-invasive fashion. Adhesion to electrodes was quantified via spreading rates derived from impedance changes, period analysis allowed us to measure contraction dynamics and modulations of the barrier resistance served as a measure of connectivity. In summary, we claim that: (i) a preferred window for substrate elasticity around 7 kPa for low fibroblast content exists, which is shifted to stiffer substrates with increasing fibroblast fractions. (ii) Beat frequency decreases nonlinearly with increasing fraction of fibroblasts, while (iii) the intercellular resistance increases with a maximal functional connectivity at 75% fibroblasts. For the first time, cardiac cell–cell junction density-dependent connectivity in co-cultures of cardiomyocytes and fibroblasts was quantified using ECIS.

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