Background: Adverse Childhood Experiences (ACEs) are linked to poor maternal mental health. By disrupting stress regulation systems, ACEs are hypothesized to impact perceived stress, anxiety, and cortisol. This study explores the associations of ACEs with different manifestations of stress. Methods: Participants were part of the Bachpan study, a longitudinal birth cohort in rural Pakistan. Data were collected at the 36-month postpartum wave. ACEs were captured retrospectively using an adapted version of the ACE International Questionaire, and represented in the following ways: as a continuous variable, binary indicator, categoric levels, and subdomains (neglect, home violence, family psychological distress, community violence). Outcomes included: perceived stress (N=889) measured with the Cohen Perceived Stress Scale (PSS), anxiety (N=623) measured with the Generalized Anixety Disorder-7 scale (GAD-7), and hair-derived cortisol (N=90). Multivariable linear mixed models estimated associations between ACEs and the outcome variables. Results: All models featured positive associations between ACE items and PSS. Both the continuous total ACE score (B=0.4; 95% CI=0.0, 0.8) and the presence of any ACEs (B=1.0; 95% CI=-1.0, 0.3) were associated with higher anxiety symptoms on the GAD-7. Home violence (B=6.7; 95% CI=2.7, 10.8) and community violence (B=7.5; 95% CI=1.4,13.6) were associated with increased hair cortisol. Conclusions: All four ACE domains were associated with elevated levels of perceived stress, anxiety, and cortisol, with varying precision and strength of estimates, indicating that the type of ACE has a differential impact. This study disentangled adversity to understand the impact of specific adverse events on hypothalamic pituitary adrenal (HPA) axis functioning and mental health conditions.