Intervertebral Disc Repair by Autologous Mesenchymal Bone Marrow Cells: A Pilot Study

Orozco, Lluís; Soler, Robert; Morera, Carles; Alberca, Mercedes; Sánchez, Abraham; García‐Sancho, Javier

doi:10.1097/tp.0b013e3182298a15

Cited by 432 publications

(429 citation statements)

References 32 publications

Supporting

Mentioning

414

Contrasting

Unclassified

Order By: Relevance

“…To assess whether continuous culture can produce clinically relevant populations of NP cells, cell doubling was calculated. Similar population doubling was found using both methods and both total cell counts were suitable for clinical applications (Mochida et al, 2015;Orozco et al, 2011) (Fig. 3B).…”

Section: Continuous Expansion Culture Preserved Gene Expression Of Ecsupporting

confidence: 51%

“…Autologous chondrocyte implantation has had some success at repairing focal cartilage defects in patients (Brittberg et al, 1994;Brittberg et al, 2001). Autologous stem cell implantation for IVD repair has shown some clinical promise (Orozco et al, 2011). Autologous NP implantation has been shown to be safe and not cause low back pain, yet NP cells insufficiently repair IVDs (Mochida et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

Rosenzweig¹,

Gravel²,

Bisson³

et al. 2017

eCM

View full text Add to dashboard Cite

Autologous NP cell implantation is a potential therapeutic avenue for intervertebral disc (IVD) degeneration. However, monolayer expansion of cells isolated from surgical samples may negatively impact matrix production by way of dedifferentiation. Previously, we have used a continuous expansion culture system to successfully preserve a chondrocyte phenotype. In this work, we hypothesised that continuous expansion culture could also preserve nucleus pulposus (NP) phenotype. We confirmed that serial passaging drove NP dedifferentiation by significantly decreasing collagen type II, aggrecan and chondroadherin (CHAD) gene expression, compared to freshly isolated cells. Proliferation, gene expression profile and matrix production in both culture conditions were compared using primary bovine NP cells. Both standard culture and continuous culture produced clinically relevant cell populations. However, continuous culture cells maintained significantly higher collagen type II, aggrecan and CHAD transcript expression levels. Also, continuous expansion cells generated greater amounts of proteoglycan, collagen type II and aggrecan protein deposition in pellet cultures. To our surprise, continuous expansion of human intervertebral disc cells -isolated from acute herniation tissue -produced less collagen type II, aggrecan and CHAD genes and proteins, compared to standard culture. Also, continuous culture of cells isolated from young non-degenerate tissue did not preserve gene and protein expression, compared to standard culture. These data indicated that primary bovine and human NP cells responded differently to continuous culture, where the positive effects observed for bovine cells did not translate to human cells. Therefore, caution must be exercised when choosing animal models and cell sources for pre-clinical studies.

show abstract

Section: Continuous Expansion Culture Preserved Gene Expression Of Ecsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

Rosenzweig¹,

Gravel²,

Bisson³

et al. 2017

eCM

View full text Add to dashboard Cite

show abstract

“…Recent studies have demonstrated that the use of growth factors, gene and stem cell therapies, and tissue engineering strategies might be feasible and effective approaches to prevent and treat IDD [1]. Indeed, very recently bone marrow mesenchymal stem cells have been used in a clinical phase I study in patients seeking BP with moderate IDD, showing safety and pain improvement [2]. However, several unsolved issues are still present in the translation of this new cell therapy to the clinic, such as the most reliable transplantation method, including the surgical route to approach the disc, and suitable animal models to test regenerative techniques.…”

Section: Introductionmentioning

confidence: 99%

The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

et al. 2013

View full text Add to dashboard Cite

Purpose To characterize in vivo the transpedicular approach (TA) as an alternative route to study intervertebral disc (IVD) regeneration strategies in a sheep model. Methods 48 IVD of 12 sheep were used. TA was performed under fluoroscopy, followed by nucleotomy (2-mm shaver resector). A polyurethane scaffold was used to repair the end-plate. X-ray and MRI images were acquired pre-, intra-and post-operatively (1, 3, 6 months). Complications were recorded. Results TA was feasible in all animals; nucleus pulposus (NP) from L1 to L5 was accessible. Nucleotomy followed by end-plate repair was achieved. Loss of NP signal intensity was shown in MRI images of the nucleotomy group. Conclusions TA is feasible in vivo, repeatable after only a short learning period and safely performed without significant morbidity. This animal model allows the study of IVD degeneration physiopathology and investigation of IVD regeneration techniques in vivo.

show abstract

“…However, concerns regarding an immune reaction to allogenic stem cells in humans have limited the utilization of such cells in clinical trials. Orozco et al [62] transplanted autologous MSCs into ten patients diagnosed with Degenerative Disk Disease [62] . They found improvements in pain and disability within three months of treatment.…”

Section: Disc Regenerationmentioning

confidence: 99%

Stem cells for spine surgery

Schroeder

Kueper

Kaplan

et al. 2015

WJSC

View full text Add to dashboard Cite

In the past few years, stem cells have become the focus of research by regenerative medicine professionals and tissue engineers. Embryonic stem cells, although capable of differentiating into cell lineages of all three germ layers, are limited in their utilization due to ethical issues. In contrast, the autologous harvest and subsequent transplantation of adult stem cells from bone marrow, adipose tissue or blood have been experimentally utilized in the treatment of a wide variety of diseases ranging from myocardial infarction to Alzheimer's disease. The physiologic consequences of stem cell transplantation and its impact on functional recovery have been studied in countless animal models and select clinical trials. Unfortunately, the bench to bedside translation of this research has been slow. Nonetheless, stem cell therapy has received the attention of spinal surgeons due to its potential benefits in the treatment of neural damage, muscle trauma, disk degeneration and its potential contribution to bone fusion.

show abstract

Intervertebral Disc Repair by Autologous Mesenchymal Bone Marrow Cells: A Pilot Study

Abstract: MSC therapy may be a valid alternative treatment for chronic back pain caused by degenerative disc disease. Advantages over current gold standards include simpler and more conservative intervention without surgery, preservation of normal biomechanics, and same or better pain relief.

Cited by 432 publications

References 32 publications

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

Comparative analysis in continuous expansion of bovine and human primary nucleus pulposus cells for tissue repair applications

The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

Stem cells for spine surgery

Contact Info

Product

Resources

About